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Broad-Spectrum Antiviral Therapeutics

Figure 1

A variety of DRACOs and controls were produced.

(A) DRACOs with different dsRNA detection and apoptosis induction domains were designed and produced. All domains were human except murine Apaf-1 (mApaf-1), and some dsRNA detection domains used PKR1–181 with vaccinia E3L dsRNA binding motif replacing PKR dsRBM 1 (NTE3L), dsRBM 2 (CTE3L), or both (2×E3L). His denotes His6 purification tag and Txd denotes PTD, TAT, or ARG transduction tag. DRACOs with transduction tags on the N-, C-, or both termini were produced. (B) This protein gel shows examples of DRACOs and negative controls that were produced. 1 µg was loaded per lane. Final yields were approximately 30 mg purified protein per liter of culture.

Figure 1