High-Resolution Quantification of Focal Adhesion Spatiotemporal Dynamics in Living Cells
(A) The majority of adhesions are born within 5 µm of the cell edge and the greatest variance in assembly rates are also observed in this 5 µm band. (B) The distribution of the distance of death location from the cell edge indicates that adhesion disassembly typically occurs along a broader band from the cell edge as compared to the position at adhesion birth. Also, the variance in disassembly rate is roughly the same regardless of the position at adhesion death. This data was collected from 21 EGFP-Paxillin cells.