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High-Resolution Quantification of Focal Adhesion Spatiotemporal Dynamics in Living Cells

Figure 1

Automating the analysis of focal adhesion images requires a multi-stage pipeline.

The first row shows several representative images of fluorescently labeled Paxillin using TIRF microscopy. In the second row, a cartoon depiction of the segmented adhesions and the cell edge are shown. Identification of the adhesions in each image allows a set of characteristic morphological and fluorescence intensity-based features to be extracted. The third row shows a single adhesion (highlighted in red) being tracked through the short sample time course. The properties of each adhesion are tracked over time, allowing the large scale dynamics of FA to be determined.

Figure 1