A Flexible Approach for Highly Multiplexed Candidate Gene Targeted Resequencing
Ten-thousand consensus length (297 bases) Alu sequences were randomly selected and aligned. The percentage of Alu sequences containing MseI, BfaI, SauIIIA and CviQI sites along the multiple alignment positions is shown. The four most prevalent restriction sites are SauIIIA sites. The two most frequent amongst these are present in 50 to 75% of the Alu sequences. We note that the alignment sequence is much longer than many individual Alu sequences because of insertions and deletions.