A Flexible Approach for Highly Multiplexed Candidate Gene Targeted Resequencing
Pre- and post-adjustment capture oligonucleotides performance of capture assays 1 and 2 are shown. Capture assay 1's target size was 102.48 Kb and this intermediate version of capture assay 2 covered 616 Kb. The Y axis shows the proportions of bases across the target whose fold-coverage can be sorted into each order of magnitude before and after capture adjustment. Nominally, we opted for a sequencing depth between 100 and 1,000 as an adequate representation. In both assays, the proportion of bases whose FC is less than 100 drops significantly; in the case of capture assay 2, the number of bases with excessively high FC has dropped significantly as well.