Proline-Rich Tyrosine Kinase 2 (Pyk2) Promotes Cell Motility of Hepatocellular Carcinoma through Induction of Epithelial to Mesenchymal Transition
(A) Pyk2 over-expression did not increase activation of STAT5b in Hep3B cells (green, white arrow). (B) Western blot analyses of protein level of phosphorylated STAT5b and Twist between Hep3B-vector and Hep3B-Pyk2 cells. (C) Suppression of Pyk2 down-regulated the nuclear localization of STAT5b (green, white arrow) in MHCC97L cells. (D) Suppression of Pyk2 down-regulated the phosphorylated STAT5b and other mesenchymal genes including Twist, N-cadherin and fibronectin analyzed by Western blot.