Proline-Rich Tyrosine Kinase 2 (Pyk2) Promotes Cell Motility of Hepatocellular Carcinoma through Induction of Epithelial to Mesenchymal Transition
(A) Effects of Pyk2 over-expression on the localization of E-cadherin in Hep3B cells. Positive staining of E-cadherin (green, white arrow) was present in the cytoplasm of the transfectants. (B) Over-expression of Pyk2 down-regulated the expression of cytokeratin and E-cadherin in Hep3B cells as shown by Western Blotting. (C) Forced expression of PRNK in MHCC97L cells restored the expression of E-cadherin (green, white arrow) on the cell membrane and cytoplasm. (D) The expression of E-cadherin and cytokeratin was increased upon suppression of Pyk2 in MHCC97L cells.