p53 Interaction with JMJD3 Results in Its Nuclear Distribution during Mouse Neural Stem Cell Differentiation
Figure 3
Enhanced recruitment of JMJD3 to the ARF locus during neural differentiation.
Mouse NSCs were subjected to ChIP analysis at 0, 6, 24 and 48 h after differentiation, using antibodies and primer sets as described under “Material and Methods”. A, Presence of JMJD3 at both promoter and exon 1 regions of ARF locus in mouse NSCs under differentiation. Relative levels of JMJD3 immune complex were calculated by assigning the arbitrary value 1 to IgG levels. B, Absence of the histone mark H3K27me3 at promoter and exon 1 regions of ARF locus in mouse NSCs under differentiation. Hoxc8 was used as positive control. The results are expressed as mean ± SEM fold change of 6 independent experiments. *p<0.05, †p<0.01 and ‡p<0.001 from IgG controls.