A High-Throughput Screen for Tuberculosis Progression
(A) After fertilization eggs are harvested, washed and distributed on injection plate. (B) Appropriate inoculum is injected in early stage embryos (up to 1,024-cell stage). (C) Injected embryos are dispensed into appropriate containers and drug screens take place between 3 and 6 dpi. (D) Groups of treated and untreated embryos are separately screened using COPAS during (when appropriate) and after drug exposure. Detailed optical analyses are performed on selected larvae.