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Autoluminescent Plants

Figure 1

PCR analyses to confirm plastid transformation with vector pCAS3-LUX-rps12/TrnV.

(A) Location of PCR primers on the vector and predicted size of junction PCR fragments. Shown are also: the rps12 and trnV plastid genes; aadA, the spectinomycin resistance gene; the lux genes. The drawing is not to scale. (B) Junction PCR fragments obtained by the primers in Fig. 1A using total cellular DNA of a transplastomic plant line as template.

Figure 1