Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells
. HEK-293 cells were tranfected with the influenza replicon and either pMono-lucNS (NCR NS) or pMono-lucM (NCR M) or pMono-lucPB1 (NCR PB1) or with pMono-lucNS and pBi-NP only (Neg. Contr.). Additionally, pRL, coding for Renilla luciferase, was cotransfected in all cases. Cells were harvested 48 hours post transfection and subjected to luciferase assay. Firefly values have been normalized to Renilla values. Data represents arithmetic mean values and standard deviation.