Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells
HEK-293 cells were either tranfected with pcDNA-luc (CMV), pGL3-control (SV40) or with the influenza replicon and pMono-lucNS (Flu Replicon) or with pMono-lucNS and pBi-NP only (Neg. Contr.). Additionally, pRL, coding for Renilla luciferase, was cotransfected in all cases. Four hours post transfection cells were transferred to 27°C or 40°C or remained at 37°C. Cells were harvested 48 hours post transfection and were subjected to luciferase assay. Firefly values have been normalized to Renilla values. Data represents arithmetic mean values and standard deviation.