A Multi-Parameter, High-Content, High-Throughput Screening Platform to Identify Natural Compounds that Modulate Insulin and Pdx1 Expression
(A–C) MIN6 cells were treated for 18 hours with multiple doses (0.2 to 2000 ng/mL) of the three purified fractions (F1, F2, F3) from the sponge extract identified as hit #3. (D) Cells were also treated with Holothurin A, which is found in similar sponges. RNA was isolated and quantitative qRT-PCR was conducted for Ins1 and Pdx1. Results were normalized to housekeeping genes (cyclophilin and beta-actin). n = 3. * P<0.05 compared to DMSO treated controls.