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Quantitative, Architectural Analysis of Immune Cell Subsets in Tumor-Draining Lymph Nodes from Breast Cancer Patients and Healthy Lymph Nodes

Figure 1

Integrated image analysis approach.

Stage 1: multicolor staining of tissue sections. Stage 2: high-resolution spectral imaging and automated scanning of the entire tissue section. Stage 3: machine-learning-based cell identification by GemIdent. Stage 4: numerical and spatial statistical analyses, such as cell densities, distances and distributions.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0012420.g001