Herpes Simplex Virus 2 ICP0− Mutant Viruses Are Avirulent and Immunogenic: Implications for a Genital Herpes Vaccine
(A) HSV-2 shedding from the eyes of mice on Day 2 following bilateral ocular inoculation with 0.8, 4, 20, or 100 thousand pfu/eye of HSV-2 MS or HSV-2 0ΔNLS (n = 8 mice per group). (B) Representative photographs of naïve mice on Day 5 following ocular inoculation with 100,000 pfu/eye of wild-type HSV-2 MS versus the HSV-2 ICP0− mutant virus, HSV-2 0ΔNLS. (C and D) Duration of survival of naïve mice following ocular inoculation with 0.8, 4, 20, or 100 thousand pfu/eye of (B) HSV-2 MS or (C) HSV-2 0ΔNLS (n = 8 mice per virus per dose; Σn = 32 mice per virus). A single asterisk (*) denotes p<0.05, and a double asterisk (**) denotes p<0.001 regarding the probability, p, that HSV-2 shedding or survival was equivalent in groups of mice inoculated with the same dose of HSV-2 MS or HSV-2 0ΔNLS.