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Herpes Simplex Virus 2 ICP0 Mutant Viruses Are Avirulent and Immunogenic: Implications for a Genital Herpes Vaccine

Figure 2

Plaque assay evaluation of interferon-sensitivity.

(A and B) Photographs of monolayers of ICP0+ L7 cells versus Vero cells treated with interferon-β (IFN-β, 200 U/ml), which were inoculated with 1.0-log dilutions of (A) wild-type HSV-2 MS or (B) HSV-2 0ΔNLS. (C) Reductions in the efficiency of plaque formation of each virus in untreated Vero cells or IFN-β-treated Vero cells relative to fully permissive monolayers of ICP0+ L7 cells (mean ± sem reduction based on n = 3 plaque assays per group). A double asterisk (**) denotes p<0.001 regarding the probability, p, that each HSV-2 ICP0 mutant virus formed plaques in IFN-β-treated Vero cells with the same efficiency as wild-type HSV-2.

Figure 2