Genetic Control of Lithium Sensitivity and Regulation of Inositol Biosynthetic Genes
A, A diagram to illustrate the gene regulatory network through which PO activity regulates expression of the IMPA1, IMPA2 and ISYNA1 genes. The gene regulatory network is separate from ligand-stimulated regulation of IP3 signalling via phospholipase C (PLC), but interacts via changes in gene expression or interchange of soluble IP3. * marks genes and enzyme activities associated with bipolar mood disorder. B, HEK293 cells were treated with either mipp1 specific or non-targetting siRNA for 24 hours with or without 130 µM Z-pro-L-prolinal (PO inhibitor). Gene expression was quantified by qRT-PCR using expression of B2M as a reference. Expression of IMPA1, IMPA2 and ISYNA1 genes was quantified as percentage relative change compared to control (DMSO carrier treated, non-targeted siRNA samples). * p<0.05, ** p<0.02, *** p<0.001.