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The MinCDJ System in Bacillus subtilis Prevents Minicell Formation by Promoting Divisome Disassembly

Figure 8

Effects of MinD overexpression on MinC and FtsA.

A. Left, MinC-GFP localization in wild type (EBS499), center: in MinD+ (SB086) induced with 1% xylose, and right, in ΔminJ (SB086). top to bottom, phase contrast, membrane stain, MinC-GFP, and a merged image of the membrane stain and MinC-GFP. MinD overexpression leads to a localization pattern of MinC-GFP with multiple rings forming throughout the cell, with double rings frequently being observed. In the absence of MinJ and overexpression of MinD, MinC-GFP becomes completely dispersed and forms foci throughout the cell. B. Left: FtsA-YFP localization in MinD+ (SB084) and right: in MinD+ ΔminJ (SB085). In both strains expression of MinD was induced with 1% xylose. From top to bottom, the figure shows an image of the membrane stain (FM4-64), FtsA-YFP localization, and a merged image of FtsA-YFP and the membrane stain. FtsA-YFP expressed in cells overexpressing MinD still localizes in wild type and ΔminJ cells indicating that the filamentous cell phenotype must occur downstream of FtsA recruitment to the Z-ring.

Figure 8