The MinCDJ System in Bacillus subtilis Prevents Minicell Formation by Promoting Divisome Disassembly
A. A series of MinJ truncations were created to test which domains are important for function. Note that all truncation were expressed as C-terminal GFP fusions. Localization of the fusion proteins can be found in supplemental material Fig. S5. These truncations include the soluble PDZ domain, TM1, containing the PDZ domain and the last transmembrane helix; TM2, with the PDZ domain and the last two transmembrane helices, and so forth. B. To test functionality, they were expressed in ΔminJ cells and the cell length and amount of minicells were measured. From left to right, strains tested were wild type (168), ΔminJ (RD021), ΔminJ MinJ+ (MB004), PDZ (SB010), TM1 (SB004), TM2 (SB005), TM3 (SB006), TM4 (SB007) and TM5 (SB008). Grey bars indicate the percentage of minicells produced, and the black bars indicate the average cell length. Expression of TM1 and TM2 led to an identical length as wild type, although they produced even more minicells than the MinJ knockout.