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The MinCDJ System in Bacillus subtilis Prevents Minicell Formation by Promoting Divisome Disassembly

Figure 5

Cells without a functional Min system form multiple minicells.

Shown are examples for ΔminCD (3309), ΔminCJ (SB074), ΔminDJ (SB075), and ΔminCDJ (MB012), with phase images and membrane stains taken for a few exemplary cells. Note the formation of 2–4 minicells in a row, indicating that a divisome that fails to disassemble often initiates a new round of division, resulting in multiple minicell formation. Scale bar is 5 µm.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0009850.g005