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The MinCDJ System in Bacillus subtilis Prevents Minicell Formation by Promoting Divisome Disassembly

Figure 2

FtsA-YFP remains associated with the cell poles in the absence of a functional Min system.

A. From top to bottom, localization of FtsA-YFP in wild type cells (SB067), ΔminCD (SB060), ΔminJ (SB066), and ΔminCDJ (SB061). Left to right: phase contrast image, membrane stain (FM4-64), FtsA-YFP, and a merged image of the membrane stain and FtsA-YFP. Scale bar is 5 µm. Arrows indicate FtsA rings resembling spirals, which are found at cell poles/late septa. B. Percentage of cell poles containing FtsA-YFP in wild type cells (SB067), ΔminCD (SB060), ΔminJ (SB066), and ΔminCDJ (SB061) (n = 200).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0009850.g002