The MinCDJ System in Bacillus subtilis Prevents Minicell Formation by Promoting Divisome Disassembly
A. Localization of MinJ-CFP, expressed from its native locus (strain SB003). From top to bottom the images show the phase contrast, membrane stain (FM4-64), MinJ-CFP, and merged image of membrane and MinJ-CFP. The scale bar is 5 µm. Three different localization patterns are shown: on the left panel, MinJ-CFP localizes at young poles/midcell (44.6%), in the middle panel, MinJ-CFP localizes to both poles (37.6%), and in the right panel, MinJ-CFP localizes to both midcell and poles (17.8%). In total 250 cells were counted. B. Time lapse microscopy of GFP-MinJ (strain MB001) showing the dynamic localization of MinJ. Numbers indicate minutes. Top shows a merged image of phase contrast and GFP-MinJ microscopy image, the bottom part shows a cartoon of localization of GFP-MinJ of one particular cell (highlighted in white box in microscopy image). The image shows that the localization of GFP-MinJ depends on the state of the cell cycle. When cells are not dividing, GFP-MinJ is localized to the poles. As cells prepare to divide, GFP-MinJ moves from the poles to midcell. After division is completed, GFP-MinJ moves back to the poles. The complete movie can be seen in the supplemental material (Movie S2).