The Anopheles gambiae Odorant Binding Protein 1 (AgamOBP1) Mediates Indole Recognition in the Antennae of Female Mosquitoes
Electroantennogram responses of Anopheles gambiae females to p-cresol (1), indole (2), 3-methyl indole (3) and geranylacetone (4) eluting from an apolar gas chromatographic column. For the top 3 traces, 20 ng quantities of p-cresol, indole, 3-methyl indole and geranylacetone were injected and for the next two traces down 2 ng of these ligands were injected. The column effluent was split (50:50) between the flame ionization detector (FID, bottom trace) of the chromatograph and the antennal preparations (control and treatments above). In recordings from the antennae of three females injected with AgamOBP1-dsRNA (treatments) the response to indole and 3-methyl indole was silenced whereas in two recordings from the antennae of a females injected with AgamOBP7-dsRNA (controls) the responses to indole and 3-methyl indole were no different to that of an uninjected female (small insert on right); mV scale common to treatment and control recordings.