Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone
Figure 2
GC-cGMP-PKG pathway during sperm chemotaxis mediated by P.
A, B and E: Percentage of oriented spermatozoa (OS) towards 10 pM P gradient after treating the cells with ODQ (15 min), LY-83.583 (15 min) and KT5823 (5 min), respectively. C: cGMP intracellular concentration in the absence or presence of 10 pM P. D: Percentage of oriented spermatozoa towards db-cGMP. HAM was assayed as a negative control. Each bar represents the mean ± SEM. a Significant differences vs. without inhibitor (p<0.05). b Significant differences vs. without P (p<0.05). c Significant differences vs. HAM (p<0.05).