Protection of Stem Cell-Derived Lymphocytes in a Primate AIDS Gene Therapy Model after In Vivo Selection
(A) Displayed is the percentage of transgene positive cells in different leukocyte subpopulations in the peripheral blood of monkey M05189 that received the C46 membrane fusion inhibitor and EGFP-expressing vector. In this macaque EGFP-expressing cells were found at significant percentages in all lineages examined. (B) Lineage-specific staining for macaques M05189 and M05270. Macaque M05189 and the control EYFP arm of M05270 had significant percentages of transgene-expressing cells in all lineages. For the experimental EGFP-expressing arm of M05270 that received the vector with the C46 fusion inhibitor and the U6-driven site I shRNA, the percentage of transgene-expressing cells was low in all lineages examined.