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A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Figure 3

Maps of the Destination vectors.

(A, C) The pLenti X1 and X2 series are promoter-less and require that the expression promoter come from the Entry vector. (B) The pLenti X2 series is designed for shRNA insertion into the 3′ LTR, resulting in insert duplication in the final integrated form of the viral genome. (D) The CMV and PGK series provide the promoters for constitutive expression, and the CMV/TO promoter for regulation of expression by doxycycline. (E) The pLenti GFP DEST allows insertion of an expression cassette after the Woodchuck post-transcriptional element (PRE) and expresses GFP to make recipient cells fluorescent. (F) Retroviral Destination vectors for RNAi where the Destination cassette was inserted in the 3′ LTR, similar to the pLenti X2 series. (G, H). Retroviral Destination vectors for cDNA expression under the control of the CMV promoter. See text, Table 1 and Figure 4 for details and compatibilities between each vector.

Figure 3