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A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Figure 1

Overview of the viral system.

A cDNA/shRNA/miRNA is cloned into an Entry vector between the attL1 and attL2 sites. In the presence of the LR clonase, recombination occurs between attL1-attR1 and attL2-attR2 to transfer the insert from the Entry vector into the Destination vector of choice. All Entry vectors contain the kanamycin resistance gene whereas all Destination vectors carry the ampicillin resistance gene.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0006529.g001