Plk1-Dependent Recruitment of γ-Tubulin Complexes to Mitotic Centrosomes Involves Multiple PCM Components
Figure 1
Plk1 activity is required for localization of γ-tubulin and GCP-WD to centrosomes and spindle microtubules.
(A) HeLa cells treated with monastrol or BI2536 were fixed and immunostained for γ-tubulin and pericentrin.DAPI was used to label DNA. (B) HeLa cells treated with monastrol, BI2536 or siRNA against Plk1 were fixed and immunostained for GCP-WD and α-tubulin. DNA was visualised using DAPI.