At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1
Figure 1
Androgenetic and Parthenogenetic PMEFs maintain appropriate imprinted gene expression.
RT-PCR was performed on indicated PMEF cell lines: W (Wild-type), A (Androgenetic), and P (Parthenogenetic). Histone acetyltransferase 1 (Hat1) is a biallelically expressed control. Dlk1 and Peg3 are controls for paternal expression while Grb10 and Meg3/Gtl2 are controls for maternal expression. With Irm primers 1307Up and 1581Dn, Irm RNA expression was undetectable in AG PMEF, while its expression was abundant in WT and PG PMEFs. Moreover, Meg8 and Meg9, two other genes in the large imprinted gene cluster that contains Irm, have expression patterns that are consistent with being imprinted with maternal expression. The PCR primers were Hat1 510Up/758Dn, Grb10 270Up/770Dn, Meg3 267Up/578Dn, Peg3 4430Up/4956Dn, Dlk1 830Up/1274Dn, Irm 988Up/1140Dn, Meg8 1Up/294Dn (AF498299) and Meg9 2614-3009.