Long Lasting Persistence of Bacillus thuringiensis Subsp. israelensis (Bti) in Mosquito Natural Habitats
A) Oil immersion microscopy observation of the vegetative cells (vc) and spores (spo) after Gram staining of isoloated toxic bacterium of leaf litter. The parasporal inclusions (pi) were observed by phase contrast microscopy. B) Fluorescent microscopy serotype analysis of Bti isolated from toxic or non toxic leaf litter. Bacilli were stained with rabbit antiserum specific for flagellar H14 serotype. Rabbit antibodies were revealed with FITC-conjugated goat anti rabbit Ig.