Comparative Analysis of Viral Gene Expression Programs during Poxvirus Infection: A Transcriptional Map of the Vaccinia and Monkeypox Genomes
Figure 8
Block of late virus transcription with cycloheximide and/or hydroxyurea.
Hierarchical clustering of 814 poxvirus elements. The level of expression of each gene, in each sample, relative to the mean expression level of that gene across all of the samples was determined. The intensity of the color reflects the magnitude of the change relative to the rest of the samples (−64 fold to 64 fold change). Mock = mock infection (media only); γ-MPXV = killed (gamma-irradiated) Monkeypox-Zaire; VACV-WR = Vaccinia-Western Reserve; MPXV = Monkeypox-Zaire; CHX = cycloheximide; CHX+VACV = VACV infected, CHX added immediately post-infection; CHX+MPXV = MPXV infected, CHX added immediately post-infection; HU+VACV = VACV infected, hydroxyurea (HU) added immediately post-infection then washed out at 2 hours post-infection and CHX added; HU+MPXV = MPXV infected, HU added immediately post-infection then washed out at 2 hours post-infection and CHX added.