Dynamics of Co-Transcriptional Pre-mRNA Folding Influences the Induction of Dystrophin Exon Skipping by Antisense Oligonucleotides
Figure 7
The efficiency of a novel AON targeting exon 57.
The left panel shows the RT-PCR analysis of dystrophin mRNA treated with novelAON57 at concentrations of 100nM, 200nM and 400nM of AONs. For every AON concentration, the relative percentage of total transcripts (average value of duplicate transfections) with skipping of the targeted exon is given below the lanes. The middle panel indicates the relative position of the novel AONs with respect to published AONs. The right panel plots the co-transcriptional binding accessibility of the novel AON target sites, wherein the horizontal axis denotes sequential steps of transcriptional analysis and the vertical axis denotes numbered nucleotides within the AON target site. At each step of transcriptional analysis, nucleotides in the target site that are engaged are depicted as a black dot in the plot.