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Open Access
Peer-reviewed
Research Article
TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
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Qian Zhang,
Affiliations Department of Neurology, and the Ministry of Education Key Laboratory of Child Development and Disorders, The Children’s Hospital of Chongqing Medical University, Chongqing 400046, China, Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Jing Wang,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Fang Deng,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Department of Cell Biology, the Third Military Medical University, Chongqing 400038, China
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Zhengjian Yan,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Yinglin Xia,
Affiliation Department of Biostatistics and Computational Biology, University of Rochester, Rochester, NY 14642, United States of America
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Zhongliang Wang,
Affiliations Department of Neurology, and the Ministry of Education Key Laboratory of Child Development and Disorders, The Children’s Hospital of Chongqing Medical University, Chongqing 400046, China, Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Jixing Ye,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, School of Bioengineering, Chongqing University, Chongqing 400044, China
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Youlin Deng,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Zhonglin Zhang,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Departments of General Surgery and Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
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Min Qiao,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Ruifang Li,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Departments of General Surgery and Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
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Sahitya K. Denduluri,
Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Qiang Wei,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Lianggong Zhao,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Department of Orthopaedic Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730000, China
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Shun Lu,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Department of Orthopaedic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250012, China
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Xin Wang,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Department of Surgery, West China Hospital of Sichuan University, Chengdu 610041, China
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Shengli Tang,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Departments of General Surgery and Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
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Hao Liu,
Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China
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Hue H. Luu,
Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Rex C. Haydon,
Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Tong-Chuan He ,
* E-mail: tche@uchicago.edu (T-CH); Dr_jiangli@hotmail.com (LJ)
Affiliations Department of Neurology, and the Ministry of Education Key Laboratory of Child Development and Disorders, The Children’s Hospital of Chongqing Medical University, Chongqing 400046, China, Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, United States of America
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Li Jiang
* E-mail: tche@uchicago.edu (T-CH); Dr_jiangli@hotmail.com (LJ)
Affiliation Department of Neurology, and the Ministry of Education Key Laboratory of Child Development and Disorders, The Children’s Hospital of Chongqing Medical University, Chongqing 400046, China
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TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
- Qian Zhang,
- Jing Wang,
- Fang Deng,
- Zhengjian Yan,
- Yinglin Xia,
- Zhongliang Wang,
- Jixing Ye,
- Youlin Deng,
- Zhonglin Zhang,
- Min Qiao
- Published: July 14, 2015
- https://doi.org/10.1371/journal.pone.0132666