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Posted by JoBu on 20 Aug 2013 at 17:46 GMT

Wonderful study. I have a question regarding the tissue preparation for LCM. Do you not rinse the slides in ddh20 prior to dehydration by ethanol and xylenes? Without a breif rinse in water, I observe a cloudy opaqueness on and around the tissue (the OCT compound?). This opaqueness is worsened by xylenes. Any thoughts?


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