Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages

Ludovic Tailleux; Simon J. Waddell; Mattia Pelizzola; Alessandra Mortellaro; Michael Withers; Antoine Tanne; Paola Ricciardi Castagnoli; Brigitte Gicquel; Neil G. Stoker; Philip D. Butcher; Maria Foti; Olivier Neyrolles

doi:10.1371/journal.pone.0001403

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Peer-reviewed

Research Article

Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages

Ludovic Tailleux ,

Contributed equally to this work with: Ludovic Tailleux, Simon J. Waddell, Mattia Pelizzola, Alessandra Mortellaro

Affiliation Institut Pasteur, Unit of Mycobacterial Genetics, Paris, France
⨯
Simon J. Waddell ,

Contributed equally to this work with: Ludovic Tailleux, Simon J. Waddell, Mattia Pelizzola, Alessandra Mortellaro

Affiliation Medical Microbiology, Division of Cellular and Molecular Medicine, St. George's University of London, London, United Kingdom
⨯
Mattia Pelizzola ,

Contributed equally to this work with: Ludovic Tailleux, Simon J. Waddell, Mattia Pelizzola, Alessandra Mortellaro

Affiliation Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy
⨯
Alessandra Mortellaro ,

Contributed equally to this work with: Ludovic Tailleux, Simon J. Waddell, Mattia Pelizzola, Alessandra Mortellaro

Affiliation Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy
⨯
Michael Withers,

Affiliation Department of Pathology and Infectious Diseases, Royal Veterinary College, London, United Kingdom
⨯
Antoine Tanne,

Current address: Departement of Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et Biologie Structurale (IPBS), Centre National de la Recherche Scientifique (CNRS), Université Paul Sabatier, UMR 5089, Toulouse, France

Affiliation Institut Pasteur, Unit of Mycobacterial Genetics, Paris, France
⨯
Paola Ricciardi Castagnoli,

Current address: Singapore Immunology Network (SIgN), Biomedical Sciences Institutes, Agency for Science, Technology and Research (A*STAR), IMMUNOS, Singapore, Singapore,

Affiliation Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy
⨯
Brigitte Gicquel,

Affiliation Institut Pasteur, Unit of Mycobacterial Genetics, Paris, France
⨯
Neil G. Stoker ,

*To whom correspondence should be addressed. E-mail: neil.stoker@rvc.ac.uk (NS); butcherp@sgul.ac.uk (PB); maria.foti@unimib.it (MF); olivier.neyrolles@ipbs.fr (ON)

Affiliation Department of Pathology and Infectious Diseases, Royal Veterinary College, London, United Kingdom
⨯
Philip D. Butcher ,

*To whom correspondence should be addressed. E-mail: neil.stoker@rvc.ac.uk (NS); butcherp@sgul.ac.uk (PB); maria.foti@unimib.it (MF); olivier.neyrolles@ipbs.fr (ON)

Affiliation Medical Microbiology, Division of Cellular and Molecular Medicine, St. George's University of London, London, United Kingdom
⨯
Maria Foti ,

*To whom correspondence should be addressed. E-mail: neil.stoker@rvc.ac.uk (NS); butcherp@sgul.ac.uk (PB); maria.foti@unimib.it (MF); olivier.neyrolles@ipbs.fr (ON)

Affiliation Department of Biotechnology and Bioscience, University of Milan-Bicocca, Milan, Italy
⨯
Olivier Neyrolles

*To whom correspondence should be addressed. E-mail: neil.stoker@rvc.ac.uk (NS); butcherp@sgul.ac.uk (PB); maria.foti@unimib.it (MF); olivier.neyrolles@ipbs.fr (ON)

Current address: Departement of Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et Biologie Structurale (IPBS), Centre National de la Recherche Scientifique (CNRS), Université Paul Sabatier, UMR 5089, Toulouse, France

Affiliation Institut Pasteur, Unit of Mycobacterial Genetics, Paris, France
⨯

Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages

Ludovic Tailleux,
Simon J. Waddell,
Mattia Pelizzola,
Alessandra Mortellaro,
Michael Withers,
Antoine Tanne,
Paola Ricciardi Castagnoli,
Brigitte Gicquel,
Neil G. Stoker,
Philip D. Butcher

Published: January 2, 2008
https://doi.org/10.1371/journal.pone.0001403

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Access to data

Posted by PLOS_ONE_Group on 04 Jan 2008 at 22:56 GMT

Gene regulation data for all analyzed eukaryotic genes are available in the publicly available GENOPOLIS database (https://gc-lab32.btbs.unimib.it/genopolisDB/html/users.php; login: Olivier.Neyrolles ; password: genopolis). Fully annotated raw and filtered M. tuberculosis microarray data has been deposited in BµG@Sbase (accession number: E-BUGS-58; http://bugs.sgul.ac.uk/E-BUGS-58) and also ArrayExpress (accession number: E-BUGS-58). Both databases are MIAME compliant. Preliminary access to the M. tuberculosis dataset is available at http://bugs.sgul.ac.uk/bugsbase using the username: journalaccount3, password: hg67Ky42B. To view microarray experiment details select E-BUGS-58 from the drop-down menu, then Find. Then click on the experiment summary tree to access protocols and raw/filtered expression data.
http://plosone.org/article/info:doi/10.1371/journal.pone.0001403#article1.body1.sec4.sec8.p1

The usernames and passwords published here were included in error. The accounts have been disabled. The files held in BµG@Sbase are publicly accessible without a password.

RE: Access to data

Jonas1 replied to PLOS_ONE_Group on 06 Dec 2010 at 13:47 GMT

Is the dataset from the human dendritic cells publicly available?

No competing interests declared.

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Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages

Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages

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Access to data

Posted by PLOS_ONE_Group on 04 Jan 2008 at 22:56 GMT

RE: Access to data

Jonas1 replied to PLOS_ONE_Group on 06 Dec 2010 at 13:47 GMT