Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

Open Access

Peer-reviewed

Research Article

Patrick D. Schloss ,

* E-mail: pschloss@umich.edu

Affiliation Department of Microbiology & Immunology, University of Michigan, Ann Arbor, Michigan, United States of America
⨯
Dirk Gevers,

Affiliation Microbial Systems & Communities, Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America
⨯
Sarah L. Westcott

Affiliation Department of Microbiology & Immunology, University of Michigan, Ann Arbor, Michigan, United States of America
⨯

Reader Comments

mother
http://plosone.org/article/info:doi/10.1371/journal.pone.0027310#article1.body1.sec4.sec1.p1

There was an error in the first heading in Materials and Methods. The correct heading is, "mothur".

No competing interests declared.

Ribosomal RNA
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Polymerase chain reaction
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Gene sequencing
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Sequence alignment
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Community structure
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Next-generation sequencing
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Microbiome
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Genomics
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