Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Open Access

Peer-reviewed

Research Article

Carola Engler ,

Contributed equally to this work with: Carola Engler, Ramona Gruetzner

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Ramona Gruetzner ,

Contributed equally to this work with: Carola Engler, Ramona Gruetzner

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Romy Kandzia,

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Sylvestre Marillonnet

* E-mail: marillonnet@icongenetics.de

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯

Reader Comments

This cloning strategy could be listed among the DNA assembly standards being evaluated by the BioBrick Foundation http://bbf.openwetware.or....

No competing interests declared.

Cloning
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Plasmid construction
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DNA cloning
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Ligases
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Polymerase chain reaction
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DNA fragment ligation
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Nucleotide sequencing
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Vector cloning
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