@article{10.1371/journal.pone.0001309, doi = {10.1371/journal.pone.0001309}, author = {So, Po-Wah AND Yu, Wei-Sheng AND Kuo, Yu-Ting AND Wasserfall, Clive AND Goldstone, Anthony P. AND Bell, Jimmy D. AND Frost, Gary}, journal = {PLOS ONE}, publisher = {Public Library of Science}, title = {Impact of Resistant Starch on Body Fat Patterning and Central Appetite Regulation}, year = {2007}, month = {12}, volume = {2}, url = {https://doi.org/10.1371/journal.pone.0001309}, pages = {1-8}, abstract = {BackgroundAdipose tissue patterning has a major influence on the risk of developing chronic disease. Environmental influences on both body fat patterning and appetite regulation are not fully understood. This study was performed to investigate the impact of resistant starch (RS) on adipose tissue deposition and central regulation of appetite in mice.Methodology and Principle FindingsForty mice were randomised to a diet supplemented with either the high resistant starch (HRS), or the readily digestible starch (LRS). Using 1H magnetic resonance (MR) methods, whole body adiposity, intrahepatocellular lipids (IHCL) and intramyocellular lipids (IMCL) were measured. Manganese-enhanced MRI (MEMRI) was used to investigate neuronal activity in hypothalamic regions involved in appetite control when fed ad libitum. At the end of the interventional period, adipocytes were isolated from epididymal adipose tissue and fasting plasma collected for hormonal and adipokine measurement. Mice on the HRS and LRS diet had similar body weights although total body adiposity, subcutaneous and visceral fat, IHCL, plasma leptin, plasma adiponectin plasma insulin/glucose ratios was significantly greater in the latter group. Adipocytes isolated from the LRS group were significantly larger and had lower insulin-stimulated glucose uptake. MEMRI data obtained from the ventromedial and paraventricular hypothalamic nuclei suggests a satiating effect of the HRS diet despite a lower energy intake.Conclusion and SignificanceDietary RS significantly impacts on adipose tissue patterning, adipocyte morphology and metabolism, glucose and insulin metabolism, as well as affecting appetite regulation, supported by changes in neuronal activity in hypothalamic appetite regulation centres which are suggestive of satiation.}, number = {12}, }