About the Authors

John A. Zebala

jzebala@syntrixbio.com

Affiliation Syntrix Biosystems, Inc., Auburn, Washington, United States of America

Alan Mundell

Affiliation Animal Dermatology Service, Edmonds, Washington, United States of America

Linda Messinger

Affiliation Veterinary Referral Center of Colorado, Englewood, Colorado, United States of America

Craig E. Griffin

Affiliation Animal Dermatology Clinic, San Diego, California, United States of America

Aaron D. Schuler

Affiliation Syntrix Biosystems, Inc., Auburn, Washington, United States of America

Stuart J. Kahn

Affiliation Syntrix Biosystems, Inc., Auburn, Washington, United States of America

Competing Interests

SJK and JAZ are employees of Syntrix Biosystems and JAZ holds stock in Syntrix Biosystems. LD-aminopterin is a product in development at Syntrix Biosystems. LM is a paid consultant for Antech Diagnostics, and has received research or speaker support from AB Science, Dechra, Greer Laboratories, Novartis Animal Health, Virbac and Zoetis. CG has been a paid consultant, speaker or received research support from the following companies: Elanco, Merck, Novartis Animal Health, Pinnaclife, Sogeval, TEVA animal health, Veterinary Allergy Reference Laboratory and Zoetis. This does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: JAZ SJK. Performed the experiments: AM LM CEG. Analyzed the data: JAZ SJK ADS. Contributed reagents/materials/analysis tools: ADS SJK. Wrote the paper: JAZ SJK. Contributed to critical revisions: AM LM CEG ADS.