Two Acidic, Anticoagulant PLA2 Isoenzymes Purified from the Venom of Monocled Cobra Naja kaouthia Exhibit Different Potency to Inhibit Thrombin and Factor Xa via Phospholipids Independent, Non-Enzymatic Mechanism

Ashis K. Mukherjee; Bhargab Kalita; Rupamoni Thakur

doi:10.1371/journal.pone.0101334

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Open Access

Peer-reviewed

Research Article

Two Acidic, Anticoagulant PLA₂ Isoenzymes Purified from the Venom of Monocled Cobra Naja kaouthia Exhibit Different Potency to Inhibit Thrombin and Factor Xa via Phospholipids Independent, Non-Enzymatic Mechanism

Ashis K. Mukherjee ,

* E-mail: akm@tezu.ernet.in

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India
⨯
Bhargab Kalita,

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India
⨯
Rupamoni Thakur

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India
⨯

Two Acidic, Anticoagulant PLA₂ Isoenzymes Purified from the Venom of Monocled Cobra Naja kaouthia Exhibit Different Potency to Inhibit Thrombin and Factor Xa via Phospholipids Independent, Non-Enzymatic Mechanism

Ashis K. Mukherjee,
Bhargab Kalita,
Rupamoni Thakur

Published: August 13, 2014
https://doi.org/10.1371/journal.pone.0101334

About the Authors

Ashis K. Mukherjee

* E-mail: akm@tezu.ernet.in

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India

Bhargab Kalita

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India

Rupamoni Thakur

Affiliation Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur, Assam, India

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AKM. Performed the experiments: AKM BK RT. Analyzed the data: AKM. Contributed reagents/materials/analysis tools: AKM. Wrote the paper: AKM.

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Thrombin
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Venoms
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Phospholipids
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Serine proteases
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Snakes
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Heparin
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Platelet aggregation
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Hydrolysis
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