A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation

C. Aaron Monroy; Duncan I. Mackie; David L. Roman

doi:10.1371/journal.pone.0062247

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Open Access

Peer-reviewed

Research Article

A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation

C. Aaron Monroy,

Affiliation Division of Medicinal and Natural Products Chemistry, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa, United States of America
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Duncan I. Mackie,

Affiliation Division of Medicinal and Natural Products Chemistry, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa, United States of America
⨯
David L. Roman

* E-mail: david-roman@uiowa.edu

Affiliations Division of Medicinal and Natural Products Chemistry, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa, United States of America, Cancer Signaling and Experimental Therapeutics Program, Holden Comprehensive Cancer Center, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States of America
⨯

A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation

C. Aaron Monroy,
Duncan I. Mackie,
David L. Roman

Published: April 23, 2013
https://doi.org/10.1371/journal.pone.0062247

About the Authors

C. Aaron Monroy

Affiliation Division of Medicinal and Natural Products Chemistry, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa, United States of America

Duncan I. Mackie

David L. Roman

* E-mail: david-roman@uiowa.edu

Affiliations Division of Medicinal and Natural Products Chemistry, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, Iowa, United States of America, Cancer Signaling and Experimental Therapeutics Program, Holden Comprehensive Cancer Center, University of Iowa Hospitals and Clinics, Iowa City, Iowa, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DLR CAM DIM. Performed the experiments: CAM DIM. Analyzed the data: DLR CAM DIM. Contributed reagents/materials/analysis tools: DLR. Wrote the paper: CAM DIM DLR.

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Library screening
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Guanosine triphosphatase
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G-protein signaling
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Phosphates
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Colorimetric assays
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GTPase signaling
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Hydrolysis
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Imidazole
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