A Reverse Time-Course Method for Transcriptional Chase Analyses of mRNA Half-Lives in Cultured Cells

Osheiza Abdulmalik; Alyssa A. Lombardi; J. Eric Russell

doi:10.1371/journal.pone.0040827

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Open Access

Peer-reviewed

Research Article

A Reverse Time-Course Method for Transcriptional Chase Analyses of mRNA Half-Lives in Cultured Cells

Osheiza Abdulmalik ,

* E-mail: abdulmalik@email.chop.edu

Affiliation Department of Pediatrics (Hematology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America
⨯
Alyssa A. Lombardi,

Affiliation Department of Medicine (Hematology-Oncology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America
⨯
J. Eric Russell

Affiliations Department of Pediatrics (Hematology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America, Department of Medicine (Hematology-Oncology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America
⨯

A Reverse Time-Course Method for Transcriptional Chase Analyses of mRNA Half-Lives in Cultured Cells

Osheiza Abdulmalik,
Alyssa A. Lombardi,
J. Eric Russell

Published: July 13, 2012
https://doi.org/10.1371/journal.pone.0040827

About the Authors

Osheiza Abdulmalik

* E-mail: abdulmalik@email.chop.edu

Affiliation Department of Pediatrics (Hematology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

Alyssa A. Lombardi

Affiliation Department of Medicine (Hematology-Oncology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

J. Eric Russell

Affiliations Department of Pediatrics (Hematology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America, Department of Medicine (Hematology-Oncology), University of Pennsylvania School of Medicine and The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: OA JER. Performed the experiments: OA AL JER. Analyzed the data: OA AL JER. Contributed reagents/materials/analysis tools: OA JER. Wrote the paper: OA JER.

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Messenger RNA
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DNA transcription
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HeLa cells
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Gene expression
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Cell cultures
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