Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin; Yu-Chieh Chen; Tzu-Ming Pan

doi:10.1371/journal.pone.0029101

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Open Access

Peer-reviewed

Research Article

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin,

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan
⨯
Yu-Chieh Chen,

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan
⨯
Tzu-Ming Pan

* E-mail: tmpan@ntu.edu.tw

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan
⨯

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin,
Yu-Chieh Chen,
Tzu-Ming Pan

Published: December 14, 2011
https://doi.org/10.1371/journal.pone.0029101

About the Authors

Chih-Hui Lin

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan

Yu-Chieh Chen

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan

Tzu-Ming Pan

* E-mail: tmpan@ntu.edu.tw

Affiliation Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: CHL TMP. Performed the experiments: YCC. Analyzed the data: CHL YCC TMP. Contributed reagents/materials/analysis tools: TMP. Wrote the paper: CHL YCC TMP.

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Polymerase chain reaction
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DNA
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Maize
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Plasmid construction
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DNA denaturation
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Measurement
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DNA isolation
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Fluorescent dyes
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