About the Authors
- Trenton L. Place
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Contributed equally to this work with: Trenton L. Place, Matthew P. Fitzgerald, Sujatha Venkataraman
Affiliation Molecular and Cellular Biology Program, The University of Iowa, Iowa City, Iowa, United States of America
- Matthew P. Fitzgerald
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Contributed equally to this work with: Trenton L. Place, Matthew P. Fitzgerald, Sujatha Venkataraman
Affiliation Free Radical and Radiation Biology Program and Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa, United States of America
- Sujatha Venkataraman
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Contributed equally to this work with: Trenton L. Place, Matthew P. Fitzgerald, Sujatha Venkataraman
Current address: Department of Pediatrics – Peds Hem-Onc, University of Denver, Denver, Colorado, United States of America
Affiliation University of Colorado Denver, Pediatrics, Aurora, Colorado, United States of America
- Sabine U. Vorrink
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Affiliation Human Toxicology Program, The University of Iowa, Iowa City, Iowa, United States of America
- Adam J. Case
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Affiliation Free Radical and Radiation Biology Program and Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa, United States of America
- Melissa L. T. Teoh
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Affiliation Free Radical and Radiation Biology Program and Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa, United States of America
- Frederick E. Domann
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* E-mail: frederick-domann@uiowa.edu
Affiliations Molecular and Cellular Biology Program, The University of Iowa, Iowa City, Iowa, United States of America, Free Radical and Radiation Biology Program and Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa, United States of America, Human Toxicology Program, The University of Iowa, Iowa City, Iowa, United States of America, Carver College of Medicine and The Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, Iowa, United States of America
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
Conceived and designed the experiments: FED. Performed the experiments: TLP MPF SV SUV AJC MLT. Analyzed the data: TLP MPF FED. Contributed reagents/materials/analysis tools: AJC MLT. Wrote the paper: TLP FED. Assisted with writing and editing: TLP MPF SUV. Provided funding resources for the work: SV MLT AJC FED. Isolated proteins from human tumor samples and all cell lines under normoxia and hypoxia; performed transfection studies for promoter reporter experiments; performed bisulfite sequencing and methylminer experiments and provided key data for figures 6a (right panel) and 6b: TLP. Extracted, quantified, and analyzed RNA and DNA from all cell lines; designed primers and performed bisulfite sequencing to determine methylation patterns. Provided key data for figures 1, 3, and 4: MPF. Extracted RNA, DNA and protein from breast cancer cell lines treated with 5-aza-dC for initial RT-PCR and western blotting experiments; provided key data for figures 1, 2, 5: SV. Extracted RNA, DNA and protein from human cell lines and tumor samples, performed bisulfite and Methylminer experiments on cell lines and tumor samples and assisted with producing real time PCR replicate data for PHD3 expression experiments: SUV. Extracted RNA and protein from prostate and renal cell lines for initial RT-PCR and western blotting experiments; provided key data for parts of figures 1 and 3: AJC. Isolated proteins for western blotting; provided reagents and expertise for western blots and trained the other authors in protein techniques; provided key data for figure 6a (left panel): MLT. Provided overall guidance and direction in all phases of experimental design, implementation, data acquisition and interpretation, and manuscript writing, editing and preparation: FED.