About the Authors

Yevgeniy A. Grigoryev

Affiliation Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America

Sunil M. Kurian

Affiliation Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America

Zafi Avnur

Affiliation Roche, Palo Alto, California, United States of America

Dominic Borie

Address Current address: Amgen, Thousand Oaks, California, United States of America

Affiliation Roche, Palo Alto, California, United States of America

Jun Deng

Address Current address: Immune Tolerance Institute, Menlo Park, California, United States of America

Affiliation Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

Daniel Campbell

Affiliations Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

Joanna Sung

Affiliation Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

Tania Nikolcheva

Affiliation Roche, Palo Alto, California, United States of America

Anthony Quinn

Affiliation Roche, Palo Alto, California, United States of America

Howard Schulman

Address Current address: Allosteros Therapeutics, Sunnyvale, California, United States of America

Affiliation Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

Stanford L. Peng

Affiliation Roche, Palo Alto, California, United States of America

Randolph Schaffer

Affiliation Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

Jonathan Fisher

Affiliation Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

Tony Mondala

Affiliation The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

Steven Head

Affiliation The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

Stuart M. Flechner

Affiliation Glickman Urological Institute, The Cleveland Clinic, Cleveland, Ohio, United States of America

Aaron B. Kantor

Address Current address: Immune Tolerance Institute, Menlo Park, California, United States of America

Affiliation Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

Christopher Marsh

Affiliations Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

Daniel R. Salomon

dsalomon@scripps.edu

Affiliations Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

Competing Interests

There are two competing interests involved in this study. 1) Roche Pharmaceuticals employed the following authors: Z. Avnur, D. Borie, T. Nikolcheva, A. Quinn, and S.L. Peng. Roche funded part of this work as an unrestricted research grant to Dr. Daniel R. Salomon, the senior author. The rest of the work was supported by NIH grant AI063603 to Dr. Salomon. These employees of Roche were scientific collaborators and involved in the development of the study design and reviewed the first part of the data generated. Roche has relinquished any patent interests or IP ownership in anything involved in this project. Dr. Salomon and his collaborating scientists at Scripps and The Cleveland Clinic have no ongoing consultant or speaker arrangements of any kind with Roche and no stock, stock options or other forms of equity in Roche. 2) PPD employed the following authors during the time this work was done: J. Deng, A.B. Kantor, and H. Schulman. PPD provided the Surroscan high throughput flow cytometry work in the manuscript and their participation was funded by Roche as a combination of fee for service, and then consultant fees for the data evaluation and presentation phase of the Roche-funded portion of the work. PPD has never had any patent, IP or other rights in this work. These competing interests do not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials as outlined at the journal website online.

Author Contributions

Conceived and designed the experiments: SMK DRS. Performed the experiments: YAG SMK DC. Analyzed the data: YAG SMK JD ABK. Contributed reagents/materials/analysis tools: ZA DB JS TN AQ HS SLP RLS JSF TM SRH SMF ABK CLM. Wrote the paper: YAG DRS.