Chagas disease is a zoonotic parasitic disease well-documented throughout the Americas and transmitted primarily by triatomine ‘kissing bug’ vectors. In acknowledgment of the successful history of vector control programs based on community participation across Latin America, we used a citizen science approach to gain novel insight into the geographic distribution, seasonal activity, and Trypanosoma cruzi infection prevalence of kissing bugs in Texas while empowering the public with information about Chagas disease.
We accepted submissions of kissing bugs encountered by the public in Texas and other states from 2013–2014 while providing educational literature about Chagas disease. In the laboratory, kissing bugs were identified to species, dissected, and tested for T. cruzi infection. A total of 1,980 triatomines were submitted to the program comprised of at least seven species, of which T. gerstaeckeri and T. sanguisuga were the most abundant (85.7% of submissions). Triatomines were most commonly collected from dog kennels and outdoor patios; Overall, 10.5% of triatomines were collected from inside the home. Triatomines were submitted from across Texas, including many counties which were not previously known to harbor kissing bugs. Kissing bugs were captured primarily throughout April-October, and peak activity occurred in June-July. Emails to our dedicated account regarding kissing bugs were more frequent in the summer months (June-August) than the rest of the year. We detected T. cruzi in 63.3% of tested bugs.
We created a kissing bug citizen science program in Texas to educate the public about Chagas disease, a vector-borne disease of humans and dogs, and to create a mechanism for the public to submit triatomine ‘kissing bug’ vectors to our research program. From May 2013 to December 2014, we designed an interactive website, distributed pamphlets, and responded to emails and phone calls from the public. This resulted in the submission of 1,980 kissing bugs, mostly collected from dog kennels and outdoor patios, expanding the geographic regions known to harbor kissing bugs in Texas and allowing insight into a cross-section of bugs of high epidemiological and veterinary relevance. Citizen submissions of kissing bugs peaked in June-July and showed 63.3% infection prevalence with Trypanosoma cruzi. Citizen science is an efficient mechanism for gaining novel insight into vector occurrence and infection.
Citation: Curtis-Robles R, Wozniak EJ, Auckland LD, Hamer GL, Hamer SA (2015) Combining Public Health Education and Disease Ecology Research: Using Citizen Science to Assess Chagas Disease Entomological Risk in Texas. PLoS Negl Trop Dis9(12): e0004235. https://doi.org/10.1371/journal.pntd.0004235
Editor: Ricardo E. Gürtler, Universidad de Buenos Aires, ARGENTINA
Received: May 6, 2015; Accepted: October 23, 2015; Published: December 10, 2015
Copyright: © 2015 Curtis-Robles et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: Data are available from the Oak Trust (Digital Repository) through Texas A&M University. Data are available at: http://hdl.handle.net/1969.1/155771.
Funding: This work was supported by the National Science Foundation Graduate Research Fellowship Program Grant No. 1252521 (RCR); Texas A&M University College of Veterinary Medicine Merit Fellow Program and Graduate Student Research Grant (RCR); Texas EcoLab Program (GLH, RCR); Texas AgriLife/Texas Veterinary Medical Diagnostic Lab Seed Grant (SAH), the TAMU One Health Grand Challenge, and the TAMU-CONACYT Collaborative Research Program (SAH). The open access publishing fees for this article have been covered by the Texas A&M University Online Access to Knowledge (OAK) Fund, supported by the University Libraries and the Office of the Vice President for Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Citizen science—the engagement of non-scientists in collecting scientific data—has long been used in ecological and wildlife research [1–3], resulting in an engaged public and providing researchers access to data from large geographic and temporal scales. We pose that citizen science is a powerful yet underutilized tool in public health, given that community engagement is recognized as a core component of many successful public health programs .
Chagas disease is a vector-borne zoonotic disease caused by the parasitic protozoan Trypanosoma cruzi. Infection with T. cruzi can result in cardiac and digestive disease in humans and dogs that may not manifest until years after infection. Disease in humans is well-documented throughout the Americas [5,6], and canine Chagas disease is well-documented in Texas [7,8]. In 2013 and 2014, the first two years in which Chagas disease was a notifiable disease in Texas, a total of 351 canine cases and 39 human cases were reported; the latter including 12 locally-acquired cases [9,10]. Colloquially referred to as ‘kissing bugs’, triatomine insects (Fig 1) are vectors of Trypanosoma cruzi. In the U.S., the highest species diversity of triatomines is found in Texas [5,11]. Triatomine bugs feed on blood during all stages of their lives, and may acquire the parasite from feeding on an infected mammal. The parasite T. cruzi is spread through the feces of the insect.
(Left to right) Triatoma protracta, the most common species in the western U.S.; Triatoma gerstaeckeri, the most common species in Texas; Triatoma sanguisuga, the most common species in the eastern U.S. Scale bar represents 25mm or approximately 1 inch.
Community-based vector surveillance has been widespread for decades as an approach to manage Chagas disease in South and Central America, through which householders monitor kissing bug presence within the home to allow for timely response with insecticide treatment. In these regions, some species of kissing bugs occupy a domestic niche (i.e., they successfully establish colonies in houses) . Diverse approaches have been employed in community-based vector surveillance programs, including the use of sensor boxes for passive detection of triatomines  and training of community leaders in monitoring for reinfestation and insecticide spraying [14,15]. Community-based collections were found to be more sensitive than the gold standard of timed manual searches for triatomine recoveries . A systematic review of Chagas disease vector control interventions across South and Central America concluded that community participatory surveillance significantly boosted vector detection probabilities above those found by vector control program staff using active searches or vector detection devices . Further, retrospective analyses of data from Argentina revealed that vector control strategies that incorporate community participation avert more human cases of disease and cost less than vertical or centralized strategies that consist of insecticide application by program staff only .
Community engagement has less commonly been used in the southern U.S. for kissing bug research and control, likely because the vector species in the southern U.S. tend not to colonize homes in the same manner as in Latin America, and insecticide spraying within the home is therefore not a widely used tool for public health protection. The first recruitment of the public in the U.S. to help collect kissing bugs was in 1941, when Dr. Sherwin F. Wood of Los Angeles City College encouraged Arizona miners to collect insects from their sleeping quarters with the recruitment slogan ‘Nab that bug at one cent each for Dr. Wood at City College to keep.’ ; this was followed by other similar efforts in the 1940s [20,21]. Subsequently, community participation in kissing bug surveillance in Tucson, Arizona [22,23] and New Orleans, Louisiana  has provided unprecedented information on vector phenology and infection in these regions. For public health and research purposes, the recruitment of submissions of kissing bugs from citizens who incidentally encounter them is an attractive option for collection given that kissing bugs are nocturnal, elusive, and difficult to collect using standardized entomological traps [11,25,26]. Akin to the widespread community-based vector surveillance programs in areas where Chagas disease is endemic across Latin America, here, we describe the implementation and results from a two-year citizen science program in Texas that provides public health education and encourages citizens to aid in the collection of kissing bugs across Texas.
Materials and Methods
In early 2013, we developed a citizen science program for Chagas disease research with priority interest in Texas. Our program provides resources for people seeking information about Chagas disease and kissing bugs in the U.S., while also requesting kissing bug samples through a variety of media: printed pamphlets (S1 File and S2 File), phone communication, an educational website (http://kissingbug.tamu.edu), solicitations on news stations, and a dedicated e-mail address (firstname.lastname@example.org). The public may submit insect photos through the website or email to be identified by our team, and are invited to submit kissing bug specimens along with associated information. Submitters are informed about Chagas disease transmission, and cautioned to not touch the insects with bare hands. We request that the bugs be captured in bags and stored in a freezer, to kill the insect before shipping. The minimum requested dataset to accompany each bug includes: date, time and location of capture and whether the bug was alive or dead. Location data were validated and geo-coded in a geographic information system (ArcMap, ESRI, Redlands, CA).
In the laboratory, kissing bugs were identified to species , measured, sexed, and dissected. Following DNA extraction (Omega Bio-tek, Norcross, GA; Qiagen, Germantown, MD), bug hindguts were tested for infection through amplification of T. cruzi satellite DNA quantitative real-time PCR that is selective for T. cruzi  for which our internal validations defined a positive sample as one with a cycle threshold value of 33 or less. This qPCR is highly sensitive with a limit of detection that approximates 0.5 parasite equivalents of DNA . Bugs known to have fed on humans were sent to the Centers for Disease Control via Texas Department of State Health Services for testing so that submitters can be in immediate contact with those who can make medical recommendations. We provided submitters with the species identification and preliminary T. cruzi infection status of their submission, which is shared with a statement about potential false positive or false negative results. Our website includes an interactive map to allow submitters to see their data contributions and examine the spatial and temporal distribution of kissing bugs submitted by the public Texas. We provided citizens with information on reducing kissing bug occurrence in dog kennels or patios outside the home, as these were the primary areas from which bugs were collected. These recommendations included turning off the outdoor lights at night, housing dogs indoors when possible, reducing woody debris or other potential bug harborage sites within the vicinity of the home/kennel, and the use of commercially-available insecticides, although none available in the United States are labeled for the control of triatomines.
From May 2013 through December 2014, we received approximately 4,000 emails that resulted in a total of 1,980 kissing bug submissions. The triatomines submitted to the program comprised at least seven species, of which T. gerstaeckeri and T. sanguisuga were most abundant (71.3 and 14.4% of submissions, respectively; Fig 1 and Table 1). Locations from which triatomines were most commonly reported to be collected include dog kennels (24.6%), patios/porches (19%), and inside homes (10.8%), followed by other locations including outside walls of homes, garages, cat sleeping areas, inside buildings, barns, pools, tents, and chicken coops. Overall, 10.8% of triatomines representing 5 species were collected from inside homes (Table 1) and the majority of submissions of adults from inside homes consisted of a single bug that was encountered. As a proportion of the number of collections of each species, T. rubida, T. lecticularia, and T. sanguisuga were most commonly captured inside homes. Regarding nymphs, 20 of the 56 (36.7%) nymphs submitted to our program were collected from inside the home (Table 1), including three submissions of more than one nymph (two submissions with two nymphs; one submission with five nymphs).
Over 99% of submissions were from Texas (1,968 kissing bugs), although we also received kissing bugs from Arizona, Florida, Louisiana, Oklahoma, and Virginia (Fig 2). In our initial program year in 2013, we received 881 kissing bugs submitted by 145 citizens. Our expanded program in 2014 resulted in the submission of 1,099 kissing bugs by 243 citizens, 13 of which had submitted bugs to our laboratory the preceding year. Whereas the majority of citizens submitted a single bug (200 individuals), many individuals submitted multiple bugs. There were 21 individuals who submitted 20 or more bugs over two years, including one individual who submitted 271 bugs. The majority of these large quantity submitters (90.5%) found bugs in the sleeping quarters of their dogs and expressed concerns about canine Chagas disease risk.
A) States from which our program received kissing bugs in 2013–2014 overlaid on historical state-level records of kissing bugs throughout the U.S. [5,29]; B) Historical county-level records of kissing bugs in Texas (1928–2006, as from ) and submissions of kissing bugs through our citizen science program (2013–2014).
Kissing bugs received by our laboratory were captured primarily throughout April-October, with the highest number of captures in June-July (Fig 3). The small number of kissing bugs (n = 11) that were captured in the winter months (November-March) were mainly collected from indoors (63.6%) and were comprised of a higher percentage of nymphs (36.4%) than submissions throughout the summer months (26.3%). In the subset of 694 kissing bugs submitted through this program that we subjected to molecular detection of T. cruzi, we detected infection in 493 bugs (63.3%). In all counties from which at least two bugs were submitted, at least one infected bug was detected.
Seasonal occurrence of the collection of kissing bugs by citizens, 2013–2014.
Since establishing a dedicated email account in late 2013, emails regarding kissing bugs were more frequent in the summer months (June-August) than the rest of the year. Periods of exceptionally high email traffic were frequently associated with newscasts and releases of online media related to Chagas disease and kissing bugs (Fig 4). Captures of non-kissing bugs (mainly reduvvids and other hemipterans; Fig 5) represented approximately 10% of photo submissions to our program. We occasionally received emails from citizens concerned about a bug bite that may be from a triatomine, sometimes accompanied by photos of the bite site. Rarely, these citizens also have collected a kissing bug. In all these cases, we put the citizen in contact with the local contact of the Texas Department of State Health Services who can investigate further and provide medical recommendations when warranted.
Number of daily emails to email@example.com from the public, 2014. Peaks generally correspond with a media event featuring Chagas disease and/or kissing bugs: A) July 17/18, Amarillo, Texas newscast and National Public Radio website article; B) July 24, articles about Chagas disease in Virginia; C) July 27, USA Today online article; D) August 2, Arkansas newscast; E) August 11, Cat Channel online article; F) August 18, Lubbock, Texas newscast. The regular pattern of decreasing and increasing activity (most noticeable throughout September) corresponds with weekends and weekdays, respectively.
Key morphologic features distinguish similar-looking insects: (A) Gray color and dorsal crest of wheel bugs (Arilus cristatus) (B) Wide, flattened back legs of leaf-footed bugs (Leptoglossus sp.) (C) Short head of squash bugs (Mozena sp.) (D) Close view of mouthparts of a kissing bug (left; thin and straight) and non-kissing bug (right; thick and curved); Photos courtesy of M. Merchant (A), P. Porter (B, C), and R. Bardin (D).
We used a citizen science approach to establish a collection of triatomine vectors nearing 2,000 specimens in order to define key periods of kissing bug activity, expand the county-level known range of kissing bugs in Texas, and ascertain infection prevalence with T. cruzi. This method of sampling provides unique insight into the specific subset of bugs in nature that are epidemiologically relevant—that is, those bugs that people are encountering during daily activities and that potentially pose the highest risk for transmission of T. cruzi . Further, the educational campaign and community engagement at the core of this initiative allow people to take an active role in understanding how to improve their health.
This two-year citizen science program has revealed a similar geographic distribution of kissing bugs in Texas as was previously documented over almost eight decades (Fig 2). Our results highlight kissing bug occurrence in central and south Texas, which were predicted to be the highest Chagas disease risk zones in a statewide risk map  and further extend potential risk zones to include north Texas. Further, the expansive occurrence data from the citizen science initiative can provide unprecedented spatial resolution to complement the limited data used in a previous state-wide effort (108 kissing bugs from 63 unique spatial cells) . We received vector submissions from six of the seven focal areas across Texas from which T. cruzi seropositive dogs were recently detected .
The peak in the collection of adult bugs occurred in June-July, with most activity occurring between April-October. While this apparent phenology certainly reflects periods of human outdoor activity given the collection method, it is congruent with the only other study of triatomine phenology in Texas which employed blacklight traps (a collection method that is independent of public outdoor activity) in a central Texas county in April-September to conclude that 83.4% of adult triatomines collections occurred in May-July . Because vector activity is a key component of human risk, detailed phenology data are useful for public health initiatives.
The detected T. cruzi infection prevalence in citizen-submitted Texas kissing bugs was 63%, and is similar to that found in previous studies of kissing bugs from Texas that were collected using other means. For example, a sample of 241 bugs, including those collected from wildlife nests and by health department employees, was characterized by 50.7% infection prevalence , and 69–82% of bugs collected from houses and dog kennels in central Texas were infected .
The analysis of citizen-collected data presents unique challenges due to observer error and sampling bias . For example, public submission programs result non-target bug species [32,33]; however this potential source of observer error is controlled for in our program by laboratory identification of all kissing bugs. The geographic breadth of submissions reflects the area over which citizens are aware of the program and able to contribute to it. While areas from which no bugs were submitted cannot be interpreted as an absence of kissing bugs, the occurrence data are useful for increasing medical and veterinary awareness for Chagas disease over an expanded region.
The longitudinal pattern of inquiries from the public revealed that emails to our citizen science account peaked after media events (Fig 3); many of these emails concerned inaccurate information on television, internet, or social media. The most common cause of confusion resulted when pictures of common bug species that share some similarity in appearance to kissing bugs, but are not vectors of T. cruzi, were displayed while discussing Chagas disease on the news. Our data demonstrate the influence of the media for increasing awareness for the citizen science initiative to contribute to the growing field of digital epidemiology .
This citizen science program has resulted in strengthened relationships among university researchers, state health departments, the Centers for Disease Control and Prevention, clinical veterinarians, medical practitioners, and the general public. Such coordinated efforts among stakeholders—including the public—for insect surveillance offer opportunities for integrated pest management, research, and the protection of human health (e.g., The Collaborative Strategy on Bed Bugs  and nuisance black fly reporting ). The collection of samples generated through this program will be available for analyses of triatomine population genetics, blood meal analyses, genetic typing of T. cruzi, and additional research pursuits to enhance our understanding of vector ecology, allowing us to further build upon state-wide and regional models of triatomine distributions and disease risk [30,37]. Given the demonstrated public health benefit of community engagement in vector surveillance and control in areas of Chagas disease endemicity across Latin America, citizen science should be promoted as a key approach for enhancing vector-borne disease research and public health protection efforts in the United States.
S1 File. Kissing bug pamphlet.
Tri-fold pamphlet about kissing bugs and Chagas diesase used in support of citizen science initiative in Texas.
We thank the hundreds of individuals who submitted kissing bugs to this program, as well as the students who have helped with submitted bugs: Ryan Pugh, Heather Forrest Fruscalzo, Alan Mai, Bryant McDowell, Faith Weeks, Adam Curtis, Michael Sanders, Melissa Castillo, Connor Grantham. Dan Goldberg in the Texas A&M University Department of Geography and the TAMU GeoInnovation Service Center provided website design, implementation, and support.
Conceived and designed the experiments: RCR GLH SAH. Performed the experiments: RCR EJW LDA GLH SAH. Analyzed the data: RCR GLH SAH. Contributed reagents/materials/analysis tools: RCR GLH SAH. Wrote the paper: RCR EJW LDA GLH SAH.
- 1. Bonney R, Shirk JL, Phillips TB, Wiggins A, Ballard HL, et al. (2014) Next steps for citizen science. Science (80) 343: 1436–1437.
- 2. Bonney R, Cooper CB, Dickinson J, Kelling S, Phillips T, et al. (2009) Citizen science: a developing tool for expanding science knowledge and scientific literacy. Bioscience 59: 977–984.
- 3. Dickinson JL, Zuckerberg B, Bonter DN (2010) Citizen science as an ecological research tool: challenges and benefits. Annu Rev Ecol Evol Syst 41: 149–172.
- 4. Principles of Community Engagement (2011). Second Edi. Health and Human Services Dept., Clinical and Translational Science Awards Consortium, Community Engagement Key Function Committee Task Force on the Principles of Community Engagement. 201 p.
- 5. Bern C, Kjos S, Yabsley MJ, Montgomery SP (2011) Trypanosoma cruzi and Chagas’ Disease in the United States. Clin Microbiol Rev 24: 655–681. pmid:21976603
- 6. World Health Organization (2015) Chagas disease in Latin America: an epidemiological update based on 2010 estimates. 33–44 p.
- 7. Kjos S, Snowden K, Craig T, Lewis B, Ronald N, et al. (2008) Distribution and characterization of canine Chagas disease in Texas. Vet Parasitol 152: 249–256. pmid:18255233
- 8. Tenney TD, Curtis-Robles R, Snowden KF, Hamer S a (2014) Shelter dogs as sentinels for Trypanosoma cruzi transmission across Texas. Emerg Infect Dis 20: 1323–1326. pmid:25062281
- 9. Texas Department of State Health Services (2015) Human Chagas Cases Reported, by County and Acquisition Method, Texas, 2013–2014.
- 10. Texas Department of State Health Services (2015) Reported Cases of Animal Chagas, Texas, 2013–2014.
- 11. Kjos SA, Snowden KF, Olson JK (2009) Biogeography and Trypanosoma cruzi infection prevalence of Chagas disease vectors in Texas, USA. Vector Borne Zoonotic Dis 9: 41–50. pmid:18800865
- 12. Zeledón R, Vargas LG (1984) The role of dirt floors and of firewood in rural dwellings in the epidemiology of Chagas’ disease in Costa Rica. Am J Trop Med Hyg 33: 232–235. pmid:6424484
- 13. Chuit R, Paulone I, Wisnivesky-Colli C, Bo R, Perez a C, et al. (1992) Result of a first step toward community-based surveillance of transmission of Chagas’ disease with appropriate technology in rural areas. Am J Trop Med Hyg 46: 444–450. pmid:1575292
- 14. Cecere M, Gürtler R, Canale D, Chuit R, Cohen J (2002) Effects of partial housing improvement and insecticide spraying on the reinfestation dynamics of Triatoma infestans in rural northwestern Argentina. Acta Trop 84: 101–116. pmid:12429427
- 15. Cardinal M, Lauricella M, Marcet P, Orozco M, Kitron U, et al. (2007) Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco. Acta Trop 103: 201–211. pmid:17686448
- 16. Dumonteil E, Ramirez-Sierra M, Ferral J, Euan-Garcia M, Chavez-Nuñez L (2009) Usefulness of community participation for the fine temporal monitoring of house infestation by non-domiciliated triatomines. J Parasitol 95: 469–471. pmid:18788882
- 17. Abad-Franch F, Vega MC, Rolón MS, Santos WS, Rojas de Arias A (2011) Community participation in Chagas disease vector surveillance: systematic review. PLoS Negl Trop Dis 5: e1207. pmid:21713022
- 18. Vazquez-Prokopec GM, Spillmann C, Zaidenberg M, Kitron U, Gürtler RE (2009) Cost-effectiveness of Chagas Disease vector control strategies in Northwestern Argentina. PLoS Negl Trop Dis 3: e363. pmid:19156190
- 19. Wood SF (1942) Observations on vectors of Chagas’ disease in the United States II. Arizona. Am J Trop Med 23: 315–320.
- 20. Davis DJ, McGregor T, DeShazo T (1943) Triatoma sanguisuga (LeConte) and Triatoma ambigua Nieva as natural carriers of Trypanosoma cruzi in Texas. Public Health Rep 58: 353–354.
- 21. Sullivan TD, McGregor T, Eads RB, Davis DJ (1949) Incidence of Trypanosoma cruzi, Chagas, in Triatoma (Hemiptera, Reduviidae) in Texas. Am J Trop Med Hyg 29: 453–458. pmid:18153045
- 22. Reisenman CE, Savary W, Cowles J, Gregory TL, Hildebrand JG (2012) The distribution and abundance of Triatomine insects, potential vectors of Chagas Disease, in a metropolitan area in southern Arizona, United States. J Med Entomol 49: 1254–1261. pmid:23270152
- 23. Reisenman CE, Lawrence G, Guerenstein PG, Gregory T, Dotson E, et al. (2010) Infection of kissing bugs with Trypanosoma cruzi, Tucson, Arizona, USA. Emerg Infect Dis 16: 400–405. pmid:20202413
- 24. Moudy RM, Michaels S, Jameson SB, Londono B, Lopez V, et al. (2014) Factors associated with peridomestic Triatoma sanguisuga (Hemiptera: Reduviidae) presence in southeastern Louisiana. J Med Entomol 51: 1043–1050. pmid:25276935
- 25. Kjos SA, Marcet PL, Yabsley MJ, Kitron U, Snowden KF, et al. (2013) Identification of bloodmeal sources and Trypanosoma cruzi infection in triatomine bugs (Hemiptera: Reduviidae) from residential settings in Texas, the United States. J Med Entomol 50: 1126–1139. pmid:24180119
- 26. Sjogren RD, Ryckman RE (1966) Epizootiology of Trypanosoma cruzi in southwestern North America. Part VIII: Nocturnal flights of Triatoma protracta (Uhler) as indicated by collections at black light traps. J Med Entomol 3: 81–92. pmid:5941571
- 27. Lent H, Wygodzinsky P (1979) Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas’ Disease. Bull Am Museum Nat Hist 163.
- 28. Duffy T, Cura CI, Ramirez JC, Abate T, Cayo NM, et al. (2013) Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples. PLoS Negl Trop Dis 7: e2000. pmid:23350002
- 29. Swanson DR (2011) New state records and distributional notes for some assassin bugs of the continental United States (Heteroptera: Reduviidae). Gt Lakes Entomol 44: 117–138.
- 30. Sarkar S, Strutz SE, Frank DM, Rivaldi C-L, Sissel B, et al. (2010) Chagas disease risk in Texas. PLoS Negl Trop Dis 4: e836. pmid:20957148
- 31. Pippin WF (1970) The biology and vector capability of Triatoma sanguisuga texana Usinger and Triatoma gerstaeckeri (Stål) compared with Rhodnius prolixus (Stål) (Hemiptera: Triatominae). J Med Entomol 7: 30–45. pmid:4907991
- 32. Dias JCP, Garcia ALR (1978) Vigilancia epidemiológica con participacion comunitaria. Un programa de enfermedad de Chagas. Boletín la Of Sanit Panam 84: 533–544.
- 33. Yoshioka K (2013) Impact of a community-based bug-hunting campaign on Chagas disease control: a case study in the department of Jalapa, Guatemala. Mem Inst Oswaldo Cruz 108: 205–211. pmid:23579801
- 34. Brownstein JS, Freifeld CC, Madoff LC (2014) Digital disease detection-harnessing the web for public health surveillance. N Engl J Med 360: 2153–2157.
- 35. United States Environmental Protection Agency (2015) Collaborative strategy on bed bugs. Available: http://www2.epa.gov/bedbugs/collaborative-strategy-bed-bugs.
- 36. Wilson RC, Leslie AW, Spadafora E, Lamp WO (2014) Identifying the Nuisance Black Flies (Diptera: Simuliidae) of Washington County, Maryland. Maryl Entomol 6: 41–48.
- 37. Garza M, Feria Arroyo TP, Casillas EA, Sanchez-Cordero V, Rivaldi C-L, et al. (2014) Projected future distributions of vectors of Trypanosoma cruzi in North America under climate change scenarios. PLoS Negl Trop Dis 8: e2818. pmid:24831117