There are several errors within the text of this article.
In the Cloning, expression and purification of L. donovani cysteine proteases a, b and c subsection of the Materials and Methods section, the sixth sentence should read: The PCR amplified fragments were separately cloned into NdeI/BamHI or HindIII/BamHI sites of bacterial expression vector pET28a (Novagen, Madison, USA). The ninth sentence of the same section should read: For clone confirmation, approximately 1 μg plasmid DNA from an individual miniprep was double digested with the appropriate restriction enzymes (NdeI and BamHI for cpa/b or BamHI and HindIII for cpc) and the digest loaded onto a 1% agarose gel, in parallel with the molecular weight marker: 1 kb DNA ladder (Fermentas, USA).
The primer sequences for cloning cpc gene given in Table S1 should be as follows:
Citation: Das A, Ali N (2015) Correction: Combining Cationic Liposomal Delivery with MPL-TDM for Cysteine Protease Cocktail Vaccination against Leishmania donovani: Evidence for Antigen Synergy and Protection. PLoS Negl Trop Dis 9(10): e0004185. doi:10.1371/journal.pntd.0004185
Published: October 20, 2015
Copyright: © 2015 Das, Ali. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited