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Correction: Combining Cationic Liposomal Delivery with MPL-TDM for Cysteine Protease Cocktail Vaccination against Leishmania donovani: Evidence for Antigen Synergy and Protection

  • Amrita Das,
  • Nahid Ali

Correction: Combining Cationic Liposomal Delivery with MPL-TDM for Cysteine Protease Cocktail Vaccination against Leishmania donovani: Evidence for Antigen Synergy and Protection

  • Amrita Das, 
  • Nahid Ali
PLOS
x

There are several errors within the text of this article.

In the Cloning, expression and purification of L. donovani cysteine proteases a, b and c subsection of the Materials and Methods section, the sixth sentence should read: The PCR amplified fragments were separately cloned into NdeI/BamHI or HindIII/BamHI sites of bacterial expression vector pET28a (Novagen, Madison, USA). The ninth sentence of the same section should read: For clone confirmation, approximately 1 μg plasmid DNA from an individual miniprep was double digested with the appropriate restriction enzymes (NdeI and BamHI for cpa/b or BamHI and HindIII for cpc) and the digest loaded onto a 1% agarose gel, in parallel with the molecular weight marker: 1 kb DNA ladder (Fermentas, USA).

The primer sequences for cloning cpc gene given in Table S1 should be as follows:

Reference

  1. 1. Das A, Ali N (2014) Combining Cationic Liposomal Delivery with MPL-TDM for Cysteine Protease Cocktail Vaccination against Leishmania donovani: Evidence for Antigen Synergy and Protection. PLoS Negl Trop Dis 8(8): e3091. doi:10.1371/journal.pntd.0003091. pmid:25144181