Control and prevention of dengue relies heavily on the application of insecticides to control dengue vector mosquitoes. In Colombia, application of the larvicide temephos to the aquatic breeding sites of Aedes aegypti is a key part of the dengue control strategy. Resistance to temephos was recently detected in the dengue-endemic city of Cucuta, leading to questions about its efficacy as a control tool. Here, we characterize the underlying mechanisms and estimate the operational impact of this resistance.
Larval bioassays of Ae. aegypti larvae from Cucuta determined the temephos LC50 to be 0.066 ppm (95% CI 0.06–0.074), approximately 15× higher than the value obtained from a susceptible laboratory colony. The efficacy of the field dose of temephos at killing this resistant Cucuta population was greatly reduced, with mortality rates <80% two weeks after application and <50% after 4 weeks. Neither biochemical assays nor partial sequencing of the ace-1 gene implicated target site resistance as the primary resistance mechanism. Synergism assays and microarray analysis suggested that metabolic mechanisms were most likely responsible for the temephos resistance. Interestingly, although the greatest synergism was observed with the carboxylesterase inhibitor, DEF, the primary candidate genes from the microarray analysis, and confirmed by quantitative PCR, were cytochrome P450 oxidases, notably CYP6N12, CYP6F3 and CYP6M11.
In Colombia, resistance to temephos in Ae. aegypti compromises the duration of its effect as a vector control tool. Several candidate genes potentially responsible for metabolic resistance to temephos were identified. Given the limited number of insecticides that are approved for vector control, future chemical-based control strategies should take into account the mechanisms underlying the resistance to discern which insecticides would likely lead to the greatest control efficacy while minimizing further selection of resistant phenotypes.
Dengue fever, caused by viruses transmitted by the Aedes aegypti mosquito, is an important threat to public health in many tropical and subtropical countries. In the absence of a vaccine or specific drug treatment, prevention and control of dengue transmission relies on interventions targeting vector mosquito populations. In the city of Cucuta, Colombia, the insecticide temephos was used for several decades to control Ae. aegypti larvae, until resistance was recently reported. In this study, the resistance to temephos in this population was quantified, and its impact on control activities estimated using simulated field trials. The mechanisms underlying the resistance were determined to be metabolic, with several key detoxification enzymes identified as potential candidates. This should be taken into account when devising future vector control and insecticide resistance management strategies in this region of Colombia.
Citation: Grisales N, Poupardin R, Gomez S, Fonseca-Gonzalez I, Ranson H, Lenhart A (2013) Temephos Resistance in Aedes aegypti in Colombia Compromises Dengue Vector Control. PLoS Negl Trop Dis 7(9): e2438. doi:10.1371/journal.pntd.0002438
Editor: Paulo Filemon Pimenta, Fundaçao Oswaldo Cruz, Brazil
Received: May 24, 2013; Accepted: August 10, 2013; Published: September 19, 2013
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: This work was funded by The Wellcome Trust as part of a Master's Training Fellowship in Public Health and Tropical Medicine for NG, grant number 090679/Z/09/Z. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Dengue fever is the most rapidly expanding arboviral disease in the world. Approximately 50 million infections occur in 100 countries annually , , and 60% of those are estimated to occur in the Americas . In Colombia, dengue is considered a major public health problem, with approximately 25 million people at risk of infection. The primary vector of dengue, the Aedes aegypti mosquito, is found in more than 90% of the national territory .
Ae. aegypti is highly anthropophilic, with markedly endophilic and endophagic behaviors; these characteristics are directly related to its high efficiency as a disease vector , . In the absence of a vaccine or effective therapeutic medications, vector control remains the only available strategy to control and prevent dengue transmission .
Many dengue vector control interventions target the immature stages of the mosquito, which breed in artificial containers in close proximity to human dwellings. The most widely used method for controlling immature Ae. aegypti is the periodic treatment of actual and potential breeding sites with chemical larvicides. The organophosphate (OP) insecticide temephos is commonly used to control immature dengue vectors due to its cost-effectiveness and community acceptance , , . As a consequence of its widespread use, resistance to temephos in Ae. aegypti has been reported in many Latin American countries, including Brazil , Cuba , El Salvador , Argentina , Bolivia , Venezuela , Peru  and Colombia . It is believed that the extent of temephos resistance is underestimated due to under-reporting and lack of surveillance .
Despite increasing reports of temephos resistance in Ae. aegypti, the molecular mechanisms underpinning it are not well-characterized. In several mosquito species of medical importance such as Anopheles gambiae, Culex pipiens and Culex tritaeniorhynchus, mutations on the acetylcholinesterase gene (ace-1) have been associated with OP resistance , , . However, no mutations at this target site have been found related to OP resistance in Ae. aegypti. The three main enzyme families involved in xenobiotic detoxification in mosquitoes, glutathione S-transferases (GST), cytochrome P450 monooxygenases (CYP450) and carboxylesterases (CE) have been associated with temephos resistance in Ae. aegypti , with elevated CE activity most widely implicated. Recently, increased activity of the esterase “A4” in Ae. aegypti was partially characterized and strongly correlated with temephos resistance ; however, its genomic identity remains unknown.
Temephos is currently one of the most commonly used insecticides in Colombia . In the densely populated, dengue endemic city of San Jose de Cucuta (‘Cucuta’), temephos was used for nearly 40 years as a routine Ae. aegypti control measure but applications ceased when resistance was recently detected. Despite the potential implications of this resistance for the efficacy of dengue vector control, neither the operational impact nor the mechanisms of temephos resistance have been characterized. In this study, we explore the mechanisms of temephos resistance in Ae. aegypti from Cucuta and estimate the impact of this resistance on the efficacy of temephos-based vector control operations.
Cucuta is a city located in the eastern range of the Andes mountains of Colombia (7°54′0″N, 72°30′0″W), at 320 meters above sea level and with an average temperature of 28°C. Since the municipal water supply is frequently interrupted, people typically store water in large ground level cement tanks, or in some cases, in plastic tanks on the roof. These containers provide abundant breeding sites for Ae. aegypti.
Ae. aegypti collections
Verbal permission was obtained from householders to conduct entomological collections on their premises in March 2010. Oviposition traps (‘ovitraps’) were placed in 500 houses, while approximately 200 houses were visited for larval collections. The houses were located in five different areas of the city which were selected due to historically high levels of dengue transmission. Larval collections were made directly by removing larvae from household water storage tanks and other breeding sites, such as cans, bottles, tires, and miscellaneous discarded items, generally located in the patio area. They were taken to the insectaries at the Biologia y Control de Enfermedades Infecciosas group at the Universidad de Antioquia in Medellin, and reared under standard conditions (temperature: 28+/−1°C; relative humidity: 75+/−5%; photoperiod: 12 hours day/night). To increase larval numbers, approximately 500 ovitraps  were placed inside houses and in backyard/patio locations. After four days, the ovitraps were retrieved and checked for eggs. Positive traps were taken to the insectary where the eggs were hatched and the offspring were reared. All field samples were pooled to create the Cucuta strain.
Three insecticide-susceptible strains of Ae. aegypti were used as controls in this study. The New Orleans (NO) strain was originally collected in the namesake city located in Louisiana, United States. The Rockefeller (RCK) strain originated in Cuba nearly a century ago, while the Bora Bora (BB) strain was collected on its namesake island in French Polynesia in the 1960s .
Insecticide susceptibility tests
Standard WHO larval bioassays were conducted to detect the level of susceptibility to temephos . Each bioassay consisted of four replicates per insecticide concentration; each replicate used twenty late 3rd/early 4th instar larvae. Eight doses of temephos (Pestanal, analytic standard) ranging from 0.01 to 0.15 ppm were tested with both the Cucuta strain and a susceptible reference strain (NO). Mortality was recorded after 24 hours of exposure. LC50 values and confidence intervals were calculated using XLSTAT software (Addinsoft, Paris, France). Given that permethrin resistance had previously been reported in this population, standard WHO larval bioassays were also conducted using 6 concentrations of permethrin: 0.0075, 0.01, 0.02, 0.03, 0.04 and 0.05 ppm.
To assess the role of the three main detoxification enzyme families in temephos resistance, larvae were exposed to either diethyl-maleate (DEM), piperonyl-butoxide (PBO) or S,S,S-tributyl phosphorotrithioate (DEF) (Sigma-Aldrich) as inhibitors of GSTs, CYP450s and CEs, respectively. Standard temephos larval bioassays with three doses ranging from 0.05 ppm to 0.15 ppm were carried out with the addition of a specified concentration of synergist: either DEM at 1 ppm, PBO at 0.3 ppm or DEF at 0.5 ppm . Each bioassay consisted of three replicates per insecticide concentration; each replicate used twenty late 3rd/early 4th instar larvae. The same assay was carried out using the NO strain as a control. Resistance ratios were calculated by dividing Cucuta values by NO values at LC50, and synergism ratios were calculated as the ratio between the LC50 obtained with each synergist and the LC50 obtained without synergists.
Temephos efficacy assay
This assay was conducted in a semi-field environment at the University of Antioquia in Medellin, Colombia. Based on the methodologies proposed by Montella et al. and Lima et al. , , white plastic buckets were filled with 15 liters of tap water, and were kept outdoors, protected from direct rain and sunlight exposure. Mesh lids secured with elastic bands were used to prevent the introduction of wild mosquitoes or detritus into the buckets during the course of the experiment. A dose of 1 ppm of temephos (Abate, Fitogranos, Bogotá, Colombia), was used to treat each container, which is equivalent to the dose applied to breeding sites by vector control personnel. There were 2 experimental groups, each with three replicates: in Group 1, 3 liters of the temephos-treated water were replaced with fresh, untreated tap water twice a week, while in Group 2, the original temephos-treated water remained for the duration of the experiment without replacement. Twice a week over a 2-month period, 20 third instar larvae from the temephos-resistant Cucuta strain (F4 generation) were introduced into each container and mortality was recorded after 24 hours. Both dead and surviving larvae were removed from the containers after counting. Simultaneously, the same methodology was carried out using larvae from the RCK susceptible reference strain. Control containers without temephos were maintained under the same conditions for both experimental groups. Water temperature and pH were recorded twice a week, before each mortality recording.
Activity levels of insensitive acetylcholinesterase (iAChE), glutathione S-transferases (GST), mixed function oxidases (MFO), α-esterases and β-esterases were tested in Cucuta larvae, with larvae from the NO strain used as a negative control. Procedures were based on mosquito-specific biochemical assay protocols reported elsewhere , , . Briefly, 30 individual larvae were homogenized in 100 µL of 0.01 M potassium phosphate buffer (KPO4), ph 7.2, and then the volume was diluted to 2 ml, and 100 µl of each sample were transferred by triplicate to a 96-well microtiter plate. For the iAChE assay, 100 µl of acetylcholine iodide (ATCH) with propoxur and 100 µl of dithio-bis2-nitrobenzoic acid were added to each well; absorbance was recorded immediately (T0) and after 10 minutes (T10) in a Varioskan Flash Multimode Reader (Thermo Scientific, Delaware, USA) at a wavelength of 414 nm. As a positive control for elevated iAChE activity, the Anopheles gambiae AKRON strain (supplied by MR4, Manassas, Virginia, USA) was used.
For the GST assay, 100 µl of reduced glutathione and 100 µl of 1-chloro-2,4 – dinitrobenzene (CDNB) were added to each well. Absorbance readings were taken at T0 and T10 at a wavelength of 340 nm. For the MFO assay, 200 µl of tetramethyl-benzidine dihydrochloride (TMBZ) prepared in methanol and 0.25 M sodium acetate buffer were added to each well, followed by 25 µl of 3% hydrogen peroxide (H2O2). The microplate was incubated at room temperature for 10 minutes before reading at a wavelength of 620 nm. To detect α- and β-esterase activity, 100 µl of α-/β-naphthyl acetate were added to each well, followed by a 20 minute incubation at room temperature. 100 µl of dianizidine were then added, followed by a 4 minute incubation, and then absorbance was read at a wavelength of 540 nm.
To avoid bias due to natural variations in the size of the larvae, the total protein content of each sample was estimated. In triplicate, 200 µl of Bradford® reagent was added to 20 µl of homogenate (diluted to 100 µL by adding KPO4 buffer) and the microplate was read at 620 nm. A standard bovine serum albumin (Sigma) calibration curve was done for comparison. All replicates showing a coefficient of variation >0.20 were discarded.
RNA extraction and cDNA synthesis.
Larvae from the Cucuta strain surviving temephos exposure at a LC90 were categorized as resistant and stored in trizol after bioassay. RNA from two pools of 10 larvae was extracted using the TRIzol/chloroform RNA extraction method, according to the manufacturer (Invitrogen, Carlsbad, USA). RNA yields were assessed using a Nanodrop ND-1000 (Thermo Scientific). After treatment with DNase (Invitrogen), a reverse transcription using Oligo-dT20 (Invitrogen) and Superscript III (Invitrogen) was done. The resulting cDNA was used as PCR templates.
Ace-1 cloning and sequencing.
Ace-1 exons 4–7 were selected for sequencing based on the presence of mutations associated with OP resistance in other mosquito species, specifically at positions G119S and F290V. Two external primers were employed in the PCR, while three pairs of internal primers were used for the sequencing (Table S1). The ace-1 PCR was carried out using Phusion® High-Fidelity DNA Polymerase (Thermo Scientific). For 30 cycles, denaturing, annealing and extension conditions were 94°C for 15 seconds, 55°C for 30 seconds and 68°C for 100 seconds, respectively.
PCR products were visualized on a 1% agarose gel and purified using a GeneJET Gel Extraction Kit (Fermentas). The Ace-1 fragments were cloned (pJET 1.2/blunt Cloning Vector, Fermentas) and purified with Minipreps (GeneJET Plasmid Miniprep Kit, Fermentas). Sequencing was performed by Macrogen (Amsterdam, the Netherlands).
Genome-wide transcriptomic analysis
RNA extraction and labeled cRNA synthesis.
RNA was extracted using the Arcturus Picopure RNA Extraction Kit® from pools of twenty larvae from 5 mosquito groups: 1) F2 generation of the Cucuta strain, unexposed to insecticide (CucU), 2) F2 generation of the Cucuta strain surviving LC60 of temephos (CucR), 3) F2 generation of the Cucuta strain surviving exposure to 0.04 ppm of permethrin (CucP), 4) NO reference strain unexposed to insecticide and 5) BB reference strain unexposed to insecticide. Because the temephos used to expose the larvae was diluted in ethanol and to minimize any non-insecticide related responses, CucU, NO and BB larvae were exposed to 1% ethanol for 24 hours prior to RNA extraction. 100 ng of each RNA sample were amplified and labeled using the low input Quick Amp Labeling Kit for 2 colours (Agilent Technologies). Each sample was labeled with Cy-3 and Cy-5 dyes in different tubes.
After labeling, cRNA was purified using Qiagen RNeasy minispin columns (Qiagen, Hilden, Germany). Quality and quantity of RNA were assessed using a Nanodrop ND-1000 (Thermo Scientific, Delaware, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, California, USA). Samples were analyzed using the 15K Agilent Aedes microarray chip (ArrayExpress accession number A-MEXP-1966), which contains probes for more than 14,320 Ae. aegypti transcripts. The CucR samples were competitively hybridized to either CucU, BB, NO or CucP. Three biological replicates were used with respective dye swapping in all experiments with the exception of CucR vs CucU in which only two biological replicates were used. Hybridization was performed over 17 hours at 65°C and 10 RPM. The microarray slides were then washed using the Agilent Microarray Hybridization Kit (Agilent Technologies), following the manufacturer's protocol.
Data acquisition and analysis.
Microarrays were scanned using an Agilent G2205B microarray scanner (Agilent Technologies). The Agilent Feature Extraction software (Agilent Technologies) was employed for spot finding and signal quantification for both Cy-3 and Cy-5 dyes. Data normalization and statistical analyses were carried out using Genespring GX software (Agilent Technologies). A Student's t-test with a baseline value of 1 (same transcription level) and Benjamini and Hochbergs' multiple test correction was used to assess the over- or under-transcription of genes. Transcripts showing a 2-fold positive or negative change and a corrected P-value<0.01 were considered differentially transcribed between the experimental groups. In the CucR vs. CucU comparison, very few genes were differentially transcribed using these criteria so the P-value cutoff was increased to 0.02 and the fold change decreased to 1.5.
To validate the microarray results, 4 genes were selected from the resulting microarray candidate gene list for quantitative real-time PCR analysis: CYP6F3, CYP6N12, CYP6M11 and acetyl coA synthetase (AAEL015010). The qPCR primers were designed based on sequences retrieved from VectorBase. cDNA was obtained from larvae from the F4 generation of the Cucuta strain exposed to temephos under the same conditions as described for the microarray experiments using the reverse transcriptase Superscript® III (Invitrogen, Carlsbad, CA, USA). Each qPCR reaction of 25 µl contained 5 µl of cDNA, 0.3 M of each specific primer (Table S2) and 12.5 µl of Fast Start SYBR Green Master Mix. To verify the specificity of the primers, melting curve analyses were conducted. Standard curves were produced from cDNA serial dilutions in order to avoid bias due to differences in PCR efficiency. To determine the fold change in each selected candidate gene, the 60S ribosomal protein L8 (AAEL000987) and the 40S ribosomal protein S7 (AAEL009496) were selected as references. The relative expression of each gene was calculated using the 2−ΔΔCT method . Statistical analyses and normalization were carried out using the Relative Expression Software Tool (REST) .
Bioassays and synergist bioassays
The LC50 of temephos for the Cucuta strain of Ae. aegypti was 0.066 ppm (95% CI 0.06–0.074), approximately 15× higher than the value for the susceptible NO strain (0.0043; 95% CI 0.004–0.005). The LC95 of the Cucuta strain was 0.18 ppm (95% CI 0.15–0.23), with a resistance ratio (RR) of 14 relative to the NO strain.
Addition of the synergist DEF increased temephos susceptibility by approximately 36× in the Cucuta strain and by 7× in the New Orleans strain (Table 1). DEM resulted in a small decrease in the LC50 in the NO strain only. No effect of PBO was observed in either strain (Table 1).
Larval bioassays using permethrin indicated that the Cucuta strain was also resistant to this insecticide, with an LC50 of 0.017 ppm (95% CI 0.015–0.019) and a RR50 of 16 relative to NO.
Temephos efficacy assay
Temephos applied at the concentration used in routine vector control activities remained effective against both the RCK reference strain and the Cucuta strain for 8 weeks, provided the water was not replaced. With water replacement, over 20% of the Cucuta mosquitoes were surviving in treated containers by 4 weeks post-treatment, and nearly 80% were surviving after 2 months (Figure 1). In contrast, 100% mortality of the susceptible RCK larvae was maintained up to week six with water replacement. Water temperature for all containers ranged between 21°C and 25°C, and pH ranged between 8.0 and 8.6 (except during the second week when it briefly decreased to 7.4).
Percent mortality of Cucuta and RCK Ae. aegypti strains at different time points after application of temephos (1 ppm), with and without water renewal. CucWR/RockWR: assays of Cucuta and RCK strains with water renewal. Cuc/Rock: assays without water renewal. 95% confidence intervals are shown.
None of the enzyme families in the Cucuta strain showed enhanced activity with the model substrates used, when compared with the NO strain. Similarly, there was no evidence of AChE insensitivity, with both the Cucuta and NO strains being equally inhibited by propoxur (Supplementary information, Figure S1).
Ace-1 partial sequencing
A 1614 bp fragment of the ace-1 gene was sequenced from two pools of 10 larvae from the Cucuta strain. No amino acid polymorphisms were identified within the strain and only a single synonymous mutation was detected (position 1423, CTA to TTA) when compared to the reference sequence in Vectorbase (AAEL000511).
To select candidate genes related to temephos resistance, genes significantly differentially expressed in each experiment were filtered as shown in Figure 2. Firstly, only genes that were differentially transcribed in both CucR-NO and CucR-BB microarrays were selected (Table S3a). This resulted in a list of genes differentially transcribed between Cucuta and both geographically distinct reference strains, thus reducing the bias introduced by differences in geographic origin of the reference strains. Then, this list was cross-referenced with the differentially transcribed genes resulting from CucR-CucU microarray. This step removed any genes that did not show differential expression between the unexposed Cucuta population and those surviving the temephos LC50 in an attempt to select for genes contributing to the temephos-resistant phenotype. The resulting gene list (Table S3b) contained 124 probes, 63 of which were upregulated in the CucR population in all 3 comparisons. This list was then further reduced by filtering out any probes that were more highly expressed in Cucuta mosquitoes surviving permethrin exposure than those surviving temephos exposure, resulting in a final list of 41 upregulated candidate genes associated with temephos resistance (Figure 2; Table 2).
In selection 1, genes differentially regulated in both CucR-NO and CucR-BB microarrays were selected. These genes were compared with differentially transcribed genes from the CucR-CucU comparison, and the subset differentially expressed in both formed selection 2. This list was then compared with the differentially transcribed genes from CucP-CucU to select the genes that were under-transcribed in CucP vs. CucR microarray, resulting in 41 candidate genes.
The most over-transcribed gene in the CucR population was a putative chymotrypsin (AAEL011230-RA), with a 30-fold positive change when compared with CucU larvae. An UDP-glucosyl/glucuronosyl transferase (AAEL003076-RA) was also highly over-transcribed in this population. Three detoxification genes belonging to the CYP6 subfamily were also present on the temephos resistance candidate list: CYP6N12, CYP6F3 and CYP6M11 (Table 2). Microarray data were submitted to ArrayExpress (accession number E-MTAB-1682).
Three of the four genes selected for validation, CYP6N12, CYP6F3 and the acetyl coA synthetase, showed similar fold changes by qPCR and microarray (Table 3). However, the over expression of CYP6M11 was not confirmed by qPCR. A correlation analysis between the qPCR and microarray data yielded a R2 value of 0.31.
In Colombia, dengue transmission is a major public health problem which has led to ongoing efforts to prevent and control dengue epidemics. As part of this effort, the National Network for Surveillance of Insecticide Resistance was created, and widespread screening of Ae. aegypti susceptibility was carried out in 2005–2008 across dengue endemic regions. Moderate to high levels of resistance were reported for all four major insecticide classes across the country , .
The principal intervention to control Ae. aegypti in Colombia is the application of temephos (Abate sand-core granules) at a concentration of 1 ppm to domestic and peridomestic water storage containers , as recommended by the WHO . Resistance to temephos in Ae. aegypti has been previously reported in Colombia , . In contrast with the findings presented here, one of these studies  detected elevated MFO and esterase activity in temephos resistant populations. The present study determined that Ae. aegypti from Cucuta were able to survive 15× higher doses of temephos than a standard susceptible strain.
Insecticide resistance can potentially compromise vector control measures. It has been reported that temephos resistance can affect the efficiency of this insecticide under both field and semi-field conditions , . Insecticide bioassays with water renewal emulate the routine water replacement carried out in zones where Ae. aegypti breeding sites are intra- or peri-domestic water storage containers, offering a better approximation of the impact insecticide resistance may have on vector control measures . The finding that, after approximately one month, the majority of Cucuta larvae survived this simulated field trial, suggests that the residual effect of routine control measures is compromised by the high level of resistance. Similar efficacy losses have been reported elsewhere in temephos resistant Ae. aegypti , , , .
Semi-field or laboratory bioassays can only detect resistance when it is already present in high frequencies in a vector population. Detecting resistance at an early stage could improve vector control efficacy by triggering the implementation of alternative control strategies pre-emptively, before resistance is present at high frequencies. In order to design appropriate diagnostic tools that can detect incipient resistance, the molecular mechanisms underlying resistant phenotypes must be characterized.
The most recognized OP target site resistance mechanism is insensitive acetylcholinesterase (iAChE). Although mutations on the gene encoding this enzyme (ace-1) have been associated with OP resistance in Culex pipiens, Culex tritaeniorhynchus, Anopheles gambiae and Anopheles albimanus , , , , this has not yet been observed in Ae. aegypti. It has been hypothesized that the absence of these mutations in this species is because some of the most common mutations, such as G119S, are unlikely to occur spontaneously . The Cucuta strain did not exhibit iAChE and no amino acid changes on the ace-1 gene were detected in temephos resistant mosquitoes. This is consistent with other findings that suggest that target-site resistance plays only a minor role in temephos resistance for Ae. aegypti .
Synergist bioassays suggested that carboxylesterases were potentially responsible for temephos resistance in the Cucuta strain. However, the biochemical assays did not detect any elevation in α- or β-esterase activity in the resistant population. There are several possible explanations for this apparent contradiction. The biochemical assays used model substrates which may not be recognized by all members of the CE family. In other insects, increased esterase activity has been associated with an amino acid alteration in a particular α-esterase , ,  which is actually associated with a decrease in activity against a model substrate. Alternatively, the synergistic activity of DEF may be unrelated to its role as a CE inhibitor. To obtain a more comprehensive picture of specific genes involved in insecticide resistance, a transcriptional analysis was performed. This approach makes no assumption about the mechanisms involved but does rely on detecting changes in gene expression, and hence would not detect resistance mechanisms that resulted from an increased affinity of an enzyme for the insecticide, for example.
Two different susceptible reference strains, NO and BB, were used to minimize the genetic variation due to biological differences between strains. After a stringent analytical pipeline (Figure 2), 41 genes were identified that met the following criteria: 1. expressed at higher levels in the Cucuta population than in both the susceptible lab strains, 2. expressed at higher levels in Cucuta mosquitoes that had survived temephos exposure than in those not exposed to temephos and 3. expressed at higher levels in Cucuta mosquitoes surviving temephos exposure than in Cucuta mosquitoes surviving permethrin exposure. This final step was included as the Cucuta population was found to be resistant to both insecticides, but in this study we were particularly interested in those genes responsible for temephos resistance. It is recognized, however, that this step will have filtered out any genes that may be involved in cross resistance to both insecticides.
The final candidate list did not contain any CEs, which is in contrast with the extensive body of literature that closely relates OP resistance with this class of detoxifying enzymes , but in agreement with the results from the biochemical assays which did not support a role for elevated esterase activity in conferring the resistant phenotype. However, the possibility that temephos resistance is related to amino-acid substitutions on specific esterase genes, as has been previously reported for several insecticides in other dipteran species , , , , cannot be discounted by the microarray results.
Three gene members of the CYP6 P450 enzyme sub-family were found related to temephos resistance in this study. CYP6M11 has been reported previously as induced in Ae. aegypti in response to xenobiotics , permethrin selection  and in larvae  and adults  of temephos-resistant strains. Although this gene was found to be upregulated in the microarray analysis, the over expression of this P450 could not be confirmed by qPCR. The genes CYP6N12 and CYP6F3, identified as temephos resistance candidates in the current study, have previously been associated with resistance to the neonicotinoid imidacloprid and permethrin , , ; CYP6N12 was also associated with tolerance to the polycyclic aromatic hydrocarbon (PAH) fluoranthene  and temephos .
It has previously been suggested that the conjugation of xenobiotics with glucose is an important detoxification pathway in insects . Although UDPGTs have been described in some medically and agriculturally important insects as allelochemical detoxifiers , , they have only been demonstrated to be involved in insecticide resistance once . Recently, high levels of UDPGT over-expression in the metabolic response of Ae. aegypti larvae to permethrin have been reported . In the present study, one UDPGT (AAEL003076-RA) was associated with temephos resistance, suggesting that further studies are warranted on this transferase gene family to confirm its role in insecticide detoxification.
Serine proteases are a group of well-studied enzymes responsible for a variety of functions such as digestion, oogenesis, immune response, blood coagulation and metamorphosis , , . In the present study, a chymotrypsin (AAEL011230-RA) was the most over transcribed gene in the temephos resistant population (30.2-fold difference between temephos survivors and non-exposed Cucuta larvae, and 87.1-fold difference between temephos survivors and NO). Although it has been reported previously that trypsins and chymotrypsins from Culex pipiens pallens are able to metabolize the pyrethroid deltamethrin , , there is no evidence so far to confirm that these enzymes can metabolize temephos. Organophosphate insecticides are also known to inhibit certain serine proteases, including chymotrypsins . Functional characterization is needed to clarify the role of the chymotrypsin reported here in temephos resistance or in temephos-permethrin cross resistance.
The application of the organophosphate temephos to breeding sites is a pillar of Ae. aegypti immature control worldwide. However, its widespread, long-term use has led to the emergence of resistance in different parts of the world. Our findings demonstrate that a high level of temephos resistance significantly impacts the performance of this insecticide by reducing its residual efficacy by more than half, which in turn impacts vector control efficiency. As such, it is critical to develop tools that can detect resistance at its earliest stages of development, before resistance reaches levels at which control efficacy is compromised. The development of such tools requires a detailed understanding of the molecular basis and mechanisms underpinning resistance to insecticides. The results of the present study provide a comprehensive analysis of temephos resistance in Ae. aegypti from Cucuta, Colombia, and provide novel insights into the mechanisms underlying temephos resistance in this important disease vector. Through deeper understandings of the interactions between genes responsible for resistance to temephos and other insecticide groups, vector control programs can design control strategies that minimize the selection of resistant phenotypes and maintain vector control efficacy in the long term. In the case of Cucuta, the public health authorities have begun implementing alternative larval control strategies, including biological control (using small fish) and the application of pyriproxyfen (a juvenile hormone analogue) to breeding sites. Ongoing monitoring of temephos resistance will yield useful information about how the large scale deployment of these alternative strategies affects temephos resistance levels and its underlying mechanisms over time.
Box plots of corrected absorbance (nm) for enzyme activity, as measured by biochemical assays. A: Alpha-esterases; B: Beta-esterases; C: Mixed function oxidases; D: Glutathione S-transferases; E: Insensitive acetylcholinesterase.
Summary of primer sequences for ace-1 PCR and sequencing. Ace1-For and Ace1-Rev were the external primers used in the PCR. All other primers were used only for sequencing.
Summary of primer sequences for quantitative real-time PCR.
a Summary of temephos resistance candidate genes resulting from the first microarray data filter, ‘Selection 1’. b. Summary of temephos resistance candidate genes resulting from the second microarray data filter, ‘Selection 2.’ c. Summary of temephos resistance candidate genes resulting from the third microarray data filter, ‘Selection 3.’
The authors thank the “Instituto Departamental de Salud de Norte de Santander” for their valuable collaboration in obtaining the biological material.
Conceived and designed the experiments: AL HR IFG NG. Performed the experiments: NG RP SG. Analyzed the data: AL HR NG RP. Contributed reagents/materials/analysis tools: AL HR IFG. Wrote the paper: NG AL HR.
- 1. Simmons CP, Farrar JJ, Nguyen vV, Wills B (2012) Dengue. N Engl J Med 366: 1423–1432.
- 2. Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, et al. (2010) Dengue: a continuing global threat. Nat Rev Microbiol 8: S7–16.
- 3. Tapia-Conyer R, Betancourt-Cravioto M, Mendez-Galvan J (2012) Dengue: an escalating public health problem in Latin America. Paediatr Int Child Health 32 Suppl 1: 14–17.
- 4. Pan American Health Organization, Ministerio de la Proteccion Social Republica de Colombia., Instituto Nacional de Salud RdC (2010) Guia para la Atencion Clinica Integral del Paciente con Dengue.
- 5. World Health Organization. (2009) Dengue guidelines for diagnosis, treatment, prevention and control : new edition. Geneva: World Health Organization. x, 147 p. p.
- 6. Scott TW, Morrison AC (2010) Vector dynamics and transmission of dengue virus: implications for dengue surveillance and prevention strategies: vector dynamics and dengue prevention. Curr Top Microbiol Immunol 338: 115–128.
- 7. Chavasse DC, Yap HH, World Health Organization. Division of Control of Tropical Diseases. (1997) Chemical methods for the control of vectors and pests of public health importance. Geneva: World Health Organization. 129 p. p.
- 8. Ranson H, Burhani J, Lumjuan N, WC BI (2009) Insecticide resistance in dengue vectors. TropIKA.net Journal 1 (1)
- 9. Lima JB, Da-Cunha MP, Da Silva RC, Galardo AK, Soares Sda S, et al. (2003) Resistance of Aedes aegypti to organophosphates in several municipalities in the State of Rio de Janeiro and Espirito Santo, Brazil. Am J Trop Med Hyg 68: 329–333.
- 10. Bisset JA, Magdalena Rodriguez M, Fernandez D, Perez O (2004) [Status of resistance to insecticides and resistance mechanisms in larvae from Playa municipality collected during the intensive campaign against Aedes aegypti in Havana City, 2001–2002]. Rev Cubana Med Trop 56: 61–66.
- 11. Lazcano JA, Rodriguez MM, San Martin JL, Romero JE, Montoya R (2009) [Assessing the insecticide resistance of an Aedes aegypti strain in El Salvador]. Rev Panam Salud Publica 26: 229–234.
- 12. Llinas GA, Seccacini E, Gardenal CN, Licastro S (2010) Current resistance status to temephos in Aedes aegypti from different regions of Argentina. Mem Inst Oswaldo Cruz 105: 113–116.
- 13. Biber PA, Duenas JR, Almeida FL, Gardenal CN, Almiron WR (2006) Laboratory evaluation of susceptibility of natural subpopulations of Aedes aegypti larvae to temephos. J Am Mosq Control Assoc 22: 408–411.
- 14. Rodriguez MM, Bisset J, de Fernandez DM, Lauzan L, Soca A (2001) Detection of insecticide resistance in Aedes aegypti (Diptera: Culicidae) from Cuba and Venezuela. J Med Entomol 38: 623–628.
- 15. Rodriguez MM, Bisset JA, Fernandez D (2007) Levels of insecticide resistance and resistance mechanisms in Aedes aegypti from some Latin American countries. J Am Mosq Control Assoc 23: 420–429.
- 16. Nabeshima T, Mori A, Kozaki T, Iwata Y, Hidoh O, et al. (2004) An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhynchus. Biochem Biophys Res Commun 313: 794–801.
- 17. Alout H, Berthomieu A, Hadjivassilis A, Weill M (2007) A new amino-acid substitution in acetylcholinesterase 1 confers insecticide resistance to Culex pipiens mosquitoes from Cyprus. Insect Biochem Mol Biol 37: 41–47.
- 18. Weill M, Malcolm C, Chandre F, Mogensen K, Berthomieu A, et al. (2004) The unique mutation in ace-1 giving high insecticide resistance is easily detectable in mosquito vectors. Insect Mol Biol 13: 1–7.
- 19. Marcombe S, Poupardin R, Darriet F, Reynaud S, Bonnet J, et al. (2009) Exploring the molecular basis of insecticide resistance in the dengue vector Aedes aegypti: a case study in Martinique Island (French West Indies). BMC Genomics 10: 494.
- 20. Rodriguez M, Bisset J, Hernandez H, Ricardo Y, French L, et al. (2012) Partial characterization of esterase activity in a temephos-resistant Aedes aegypti strain. Rev Cubana Med Trop 64: 256–267.
- 21. Ocampo CB, Salazar-Terreros MJ, Mina NJ, McAllister J, Brogdon W (2011) Insecticide resistance status of Aedes aegypti in 10 localities in Colombia. Acta Trop 118: 37–44.
- 22. Lenhart AE, Walle M, Cedillo H, Kroeger A (2005) Building a better ovitrap for detecting Aedes aegypti oviposition. Acta Trop 96: 56–59.
- 23. Kuno G (2010) Early history of laboratory breeding of Aedes aegypti (Diptera: Culicidae) focusing on the origins and use of selected strains. J Med Entomol 47: 957–971.
- 24. World Health Organization. Division of Vector Biology and Control. (1981) Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. Geneva: World Health Organization. 6 p. p.
- 25. Riaz MA, Chandor-Proust A, Dauphin-Villemant C, Poupardin R, Jones CM, et al. (2013) Molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti. Aquat Toxicol 126: 326–337.
- 26. Montella IR, Martins AJ, Viana-Medeiros PF, Lima JB, Braga IA, et al. (2007) Insecticide resistance mechanisms of Brazilian Aedes aegypti populations from 2001 to 2004. Am J Trop Med Hyg 77: 467–477.
- 27. Lima EP, Paiva MH, de Araujo AP, da Silva EV, da Silva UM, et al. (2011) Insecticide resistance in Aedes aegypti populations from Ceara, Brazil. Parasit Vectors 4: 5.
- 28. Brogdon WG (1984) Mosquito protein microassay. I. Protein determinations from small portions of single-mosquito homogenates. Comp Biochem Physiol B 79: 457–459.
- 29. Brogdon WG (1988) Microassay of acetylcholinesterase activity in small portions of single mosquito homogenates. Comp Biochem Physiol C 90: 145–150.
- 30. Polson KA, Brogdon WG, Rawlins SC, Chadee DD (2011) Characterization of insecticide resistance in Trinidadian strains of Aedes aegypti mosquitoes. Acta Trop 117: 31–38.
- 31. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402–408.
- 32. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30: e36.
- 33. Fonseca-Gonzalez I, Quinones ML, Lenhart A, Brogdon WG (2011) Insecticide resistance status of Aedes aegypti (L.) from Colombia. Pest Manag Sci 67: 430–437.
- 34. Colombia MdlPSRd Instituto Nacional de Salud RdC, Organization. PAH (2012) Gestion para la vigilancia entomologica y control de la transmision del dengue. 1–126.
- 35. Maestre R, Rey G, De Las Salas J, Vergara C, Santacoloma L, et al. (2009) Susceptibility of Aedes aegypti (Diptera: Culicidae) to temephos in Atlántico-Colombia. Rev Colomb Entomol 35: 202–205.
- 36. Pinheiro VC, Tadei WP (2002) Evaluation of the residual effect of temephos on Aedes aegypti (Diptera, Culicidae) larvae in artificial containers in Manaus, Amazonas State, Brazil. Cad Saude Publica 18: 1529–1536.
- 37. Camargo Donalisio M, Leite O, Mayo R, Pinheiro Alves M, de Souza A, et al. (2002) Use of Temephos for Control of Field Population of Aedes aegypti in Americana Sao paulo, Brazil. Dengue Bulletin 26: 173–177.
- 38. Pontes RJ, Regazzi AC, Lima JW, Kerr-Pontes LR (2005) [Residual effect of commercial applications of larvicides temefos and Bacillus thuringiensis israelensis on Aedes aegypti larvae in recipients with water renewal]. Rev Soc Bras Med Trop 38: 316–321.
- 39. Garelli FM, Espinosa MO, Weinberg D, Trinelli MA, Gurtler RE (2011) Water Use Practices Limit the Effectiveness of a Temephos-Based Aedes aegypti Larval Control Program in Northern Argentina. PLoS Negl Trop Dis 5: e991.
- 40. Weill M, Fort P, Berthomieu A, Dubois MP, Pasteur N, et al. (2002) A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is non-homologous to the ace gene in Drosophila. Proc Biol Sci 269: 2007–2016.
- 41. Weill M, Berthomieu A, Berticat C, Lutfalla G, Negre V, et al. (2004) Insecticide resistance: a silent base prediction. Curr Biol 14: R552–553.
- 42. Newcomb RD, Campbell PM, Ollis DL, Cheah E, Russell RJ, et al. (1997) A single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly. Proc Natl Acad Sci U S A 94: 7464–7468.
- 43. Claudianos C, Russell RJ, Oakeshott JG (1999) The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly. Insect Biochem Mol Biol 29: 675–686.
- 44. de Carvalho RA, Torres TT, de Azeredo-Espin AM (2006) A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles. Vet Parasitol 140: 344–351.
- 45. Hemingway J, Karunaratne SH (1998) Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechanism. Med Vet Entomol 12: 1–12.
- 46. Oakeshott JG, Devonshire AL, Claudianos C, Sutherland TD, Horne I, et al. (2005) Comparing the organophosphorus and carbamate insecticide resistance mutations in cholin- and carboxyl-esterases. Chem Biol Interact 157–158: 269–275.
- 47. Cui F, Qu H, Cong J, Liu XL, Qiao CL (2007) Do mosquitoes acquire organophosphate resistance by functional changes in carboxylesterases? FASEB J 21: 3584–3591.
- 48. Cui F, Lin Z, Wang H, Liu S, Chang H, et al. (2011) Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects. Insect Biochem Mol Biol 41: 1–8.
- 49. David JP, Coissac E, Melodelima C, Poupardin R, Riaz MA, et al. (2010) Transcriptome response to pollutants and insecticides in the dengue vector Aedes aegypti using next-generation sequencing technology. BMC Genomics 11: 216.
- 50. Poupardin R, Riaz MA, Jones CM, Chandor-Proust A, Reynaud S, et al. (2012) Do pollutants affect insecticide-driven gene selection in mosquitoes? Experimental evidence from transcriptomics. Aquat Toxicol 114–115: 49–57.
- 51. Marcombe S, Mathieu RB, Pocquet N, Riaz MA, Poupardin R, et al. (2012) Insecticide resistance in the dengue vector Aedes aegypti from Martinique: distribution, mechanisms and relations with environmental factors. PLoS One 7: e30989.
- 52. Riaz MA, Chandor-Proust A, Dauphin-Villemant C, Poupardin R, Jones CM, et al. (2012) Molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti. Aquat Toxicol 126: 326–37.
- 53. Poupardin R, Reynaud S, Strode C, Ranson H, Vontas J, et al. (2008) Cross-induction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti: impact on larval tolerance to chemical insecticides. Insect Biochem Mol Biol 38: 540–551.
- 54. Strode C, de Melo-Santos M, Magalhaes T, Araujo A, Ayres C (2012) Expression profile of genes during resistance reversal in a temephos selected strain of the dengue vector, Aedes aegypti. PLoS One 7: e39439.
- 55. Leszczynski B, Matok H, Dixon AF (1992) Resistance of cereals to aphids: the interaction between hydroxamic acids and udp-glucose transferases in the aphid Sitobion avenae (Homoptera, Aphididae). J Chem Ecol 18: 1189–1200.
- 56. Bull DL, Whitten CJ (1972) Factors influencing organophosphorus insecticide resistance in tobacco budworms. J Agric Food Chem 20: 561–564.
- 57. Huang FF, Chai CL, Zhang Z, Liu ZH, Dai FY, et al. (2008) The UDP-glucosyltransferase multigene family in Bombyx mori. BMC Genomics 9: 563.
- 58. Terra W, Ferreira C (1994) Insect digestive enzymes: properties, compartmentalization and function. Comp Biochem Physiol 109: 1–62.
- 59. Krem MM, Rose T, Di Cera E (2000) Sequence determinants of function and evolution in serine proteases. Trends Cardiovasc Med 10: 171–176.
- 60. Mesquita-Rodrigues C, Saboia-Vahia L, Cuervo P, Levy CM, Honorio NA, et al. (2011) Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae). Arch Insect Biochem Physiol 76: 223–235.
- 61. Yang Q, Zhou D, Sun L, Zhang D, Qian J, et al. (2008) Expression and characterization of two pesticide resistance-associated serine protease genes (NYD-tr and NYD-ch) from Culex pipiens pallens for metabolism of deltamethrin. Parasitol Res 103: 507–516.
- 62. Yang Q, Sun L, Zhang D, Qian J, Sun Y, et al. (2008) Partial characterization of deltamethrin metabolism catalyzed by chymotrypsin. Toxicol In Vitro 22: 1528–1533.
- 63. Schaffer N, Lang RP, Simet L, Drisko RW (1958) Phosphopeptides from acid-hydrolyzed P32-labeled isopropyl methylphosphonofluoridate-inactivated trypsin. J Biol Chem 1: 185–192.