Urogenital schistosomiasis is caused by the helminth parasite Schistosoma haematobium. In high transmission areas, children acquire schistosome infection early in life with infection levels peaking in early childhood and subsequently declining in late childhood. This age-related infection profile is thought to result from the gradual development of protective acquired immunity. Age-related differences in schistosome-specific humoral and cellular responses have been reported from several field studies. However there has not yet been a systematic study of the age-related changes in human dendritic cells, the drivers of T cell polarisation.
Peripheral blood mononuclear cells were obtained from a cohort of 61 Zimbabwean aged 5–45 years with a S. haematobium prevalence of 47.5%. Two subsets of dendritic cells, myeloid and plasmacytoid dentritic cells (mDCs and pDCs), were analyzed by flow cytometry.
In this population, schistosome infection levels peaked in the youngest age group (5–9 years), and declined in late childhood and adulthood (10+ years). The proportions of both mDCs and pDCs varied with age. However, for mDCs the age profile depended on host infection status. In the youngest age group infected people had enhanced proportions of mDCs as well as lower levels of HLA-DR on mDCs than un-infected people. In the older age groups (10–13 and 14–45 years) infected people had lower proportions of mDCs compared to un-infected individuals, but no infection status-related differences were observed in their levels of HLA-DR. Moreover mDC proportions correlated with levels of schistosome-specific IgG, which can be associated with protective immunity. In contrast proportions of pDCs varied with host age, but not with infection status.
Our results show that dendritic cell proportions and activation in a human population living in schistosome-endemic areas vary with host age reflecting differences in cumulative history of exposure to schistosome infection.
A characteristic feature of most helminth infections is the convex age infection profile, where infection levels rise to peak in early childhood and decline in adulthood, a pattern thought to result from the development of protective acquired immunity. Thus, several investigations characterizing protective responses to inform vaccine research have focused on responses present in older people, who despite continued exposure to infection carry little or no infection. To date, such studies have identified key responses which are correlates of resistance. However, there is a paucity of information on cell types that are mediators rather than effectors of the immune responses. One such group where there are limited studies in human schistosome infections is dendritic cells which are important for the polarizations of CD4+ T cell responses. Therefore, we characterized the age profile of dendritic cells in Zimbabweans exposed to Schistosoma haematobium infection. We found an age-related pattern in the proportions of myeloid dendritic cells (a subset of dendritic cells) in this population. Furthermore, in the case myeloid dendritic cells, the age profile differed between schistosome infected and un-infected people. Thus our study suggests that activation and migration of myeloid dendritic cells also develop in an age-related pattern consistent with the cumulative history of exposure to schistosome parasites.
Citation: Nausch N, Louis D, Lantz O, Peguillet I, Trottein F, Chen IYD, et al. (2012) Age-Related Patterns in Human Myeloid Dendritic Cell Populations in People Exposed to Schistosoma haematobium Infection. PLoS Negl Trop Dis 6(9): e1824. doi:10.1371/journal.pntd.0001824
Editor: Rodrigo Correa-Oliveira, René Rachou Research Center, Brazil
Received: October 13, 2011; Accepted: August 6, 2012; Published: September 27, 2012
Copyright: © Nausch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the World Health Organisation (grant no. RPC264), the Wellcome Trust (grant no. WT082028MA; www.wellcome.co.uk) and by Thrasher Research Funds (www.thrasherresearch.org). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Schistosoma haematobium helminth parasites cause urogenital schistosomiasis which affects about 112 million people mainly in rural areas of subtropical countries . Infections with S. haematobium are most common in school-age children. Populations in endemic areas show a characteristic age-infection profile with infection levels increasing to peak in early to late childhood, typically around 9–15 years and then declining in adulthood –. This profile is believed to be largely reflective of the gradual development of protective acquired immunity reducing re-infection levels , , . The consequence of this age profile is that individuals with comparable infection levels who have resided in a schistosome endemic area since birth (e.g. egg negative children versus egg negative adults) can differ significantly in their immune response against the parasite and thus their levels or resistance to re-infection. Several studies characterizing human immune responses to schistosome infections have shown age related differences in antibody levels –, plasma cytokines , parasite-specific cytokines  and regulatory T cell proportions . This concept is supported by theoretical modelling of the development of acquired immunity, which predicts that correlations between immunological responses and infection is positive in younger age groups and subsequently decreasing and potentially turning in a negative correlation . Moreover this pattern is not restricted to schistosome infections, but is a characteristic of immune responses associated with protection from a variety of helminth species in endemically-exposed populations , .
As investigations of the nature and development of protective acquired immunity progress, there has been a move to decipher the mechanisms and pathways behind these observed age-related patterns. Human field studies show that the immune response against schistosomes is characterized by a very complex interaction of TH1, TH2 and regulatory responses –, – with differences between natural human infections and experimental mouse models , . Nevertheless, the induction of a TH2 response is important in the immune response against schistosomes  and in particular for the development of protection . As a major antigen-presenting cell population dendritic cells (DCs) are responsible for acquiring, processing and presenting parasite antigens to T cells and the latter interaction leads to activation and polarisation of the acquired immune response. The importance of DCs in the induction of the TH2 immune responses in the context of schistosome infections has been recently highlighted in an experimental mouse model . This study indicates that DCs are required for the induction and development of TH2 responses during schistosome infections supporting other studies showing the importance of DCs in the context of TH2 induction –.
It has been shown that schistosome derived compounds can activate dendritic cells by toll-like receptors (TLR)-2 and 3 ,  and early TH2-promoing activities can be diminished by TLR-3 . Overall studies are rare addressing the role of the innate immune cells in the immune response against schistosomes .
In particular, studies analysing DCs directly from humans exposed to schistosomes are limited. The first study addressing this question, published by Everts and colleagues in 2010, elegantly showed changes in DCs during chronic schistosomiasis (S. haematobium)  suggesting that chronic schistosomiasis can suppress DCs, which might play a role in immune modulation by schistosomes.
Peripheral blood DCs can be divided in two major subsets , , myeloid dendritic cells (mDC; or conventional DC) and plasmacytoid (pDC). Myeloid DCs express CD11c, but low levels of CD123 and have a more pronounced role in antigen processing and initiation of T cell response . Plasmacytoid DCs express CD303 (BDCA-2), ILT7, high levels of CD123 (IL-3Rα), but are negative for the classical marker CD11c , –. These pDCs play a critical role in anti-viral immunity as well as in immune tolerance , . Everts et al's  study focusing on people aged 17–39 years showed that the frequencies of mDCs and pDCs are reduced in infected people compared to un-infected and that mDCs are functionally impaired in response to toll-like receptor ligands and in driving T cell responses ex vivo. Since in most schistosome endemic areas people are exposed to infection from as young as 6 months old , and may already be carrying heavy infections within the first decade of life , these early infection events may have a profound effect on the proportions of the DCs present in the host. Characterising the role of these cells during natural immune responses to helminth infection in the different age groups is vital for vaccine development, since people targeted by anti-schistosome vaccines in endemic areas will have been previously exposed to infection and their cellular immune responses will already be primed by repeated exposure to parasite antigens. In this context variations in antigen presenting cell populations, including DCs, might directly affect the efficacy of vaccination in different age groups. Lessons from the discontinued human hookworm vaccine trials, illustrate the importance of characterising existing natural immune responses in endemic populations and designing vaccine to avoid undesirable pathological outcomes of vaccination . Therefore, the aim of this study was to characterise the relationship between age and DCs during natural schistosome infections and determine if these patterns are affected by host infection status across different age groups reflecting different dynamics in the acquisition and loss of schistosome infection.
Study population and parasitology
This study was performed in Chipinda village which is located in the Mashonaland East Province in Zimbabwe (31°94′E; 17°67′S). The village was selected because health surveys regularly conducted in the region showed little or no infection with soil-transmitted helminths (STH) and a low S. mansoni prevalence (<2%), which elicit immune responses that cross-react with those against S. haematobium , , . The low STH and S. mansoni prevalence is consistent with earlier surveys in this area of Zimbabwe –. Villagers are subsistence farmers who have frequent contact with water sources posing a risk of infection (as assessed by questionnaires) due to insufficient safe water provision and low coverage of sanitation facilities as is typical in rural Zimbabwe. Drinking water is collected from open wells while bathing and washing is conducted in perennial rivers surrounding the village. This area has not been included in any Schistosome Control Programmes and therefore participants had not received any anti-helminthic treatment for schistosomiasis or other helminth infections prior to this study. Thus their natural immune responses could be assessed in the absence of drug-altered schistosome-specific responses , . Urine and stool samples were collected on three consecutive days and examined microscopically for S. haematobium (urine filtration, Mott method ) or S. mansoni and intestinal helminths (Kato-Katz method ) respectively using standard procedures. Participants were screened for malaria by microscopic examination of Giemsa stained blood smears and HIV status was determined by immunochromatography (DoubleCheckGold™ HIV 1&2, Orgenics) with HIV positive samples subsequently re-tested by a second rapid assay (Determine HIV 1/2 Ag/Ab Combo, InvernessMedical) to confirm HIV status . Study participants had to meet the following criteria: (1) be life-long residents in this area (assessed by questionnaires) so that age would be a proxy for duration of exposure to S. haematobium infection, (2) should not have received anti-helminthic treatment prior to this study, (3) should have provided at least two urine and two stool samples on consecutive days for parasitological diagnosis, (4) should have tested negative for intestinal helminths including S. mansoni to focus on single infections with S. haematobium, (5) should be negative for HIV and malaria (Prevalence of both HIV and malaria were too small for inclusion in the statistical analysis (HIV prevalence in Chipinda village was 8% and no-one was positive for malaria infection at the time of sampling) and (6) have provided a sufficient volume of blood to isolate peripheral blood mononuclear cells (PBMC). The selected cohort comprised 61 individuals and details to the cohort are provided in Table 1.
Permission to conduct the study in the region was obtained from the Provincial Medical Director and institutional and ethical approval was received from the University of Zimbabwe's Institute Review Board and the Medical Research Council of Zimbabwe respectively. Only compliant participants were recruited and they were free to drop out at any point during the study. At the beginning of the study, participants and their parents/guardians (in case of children) had the aims and procedures of the project explained fully in the local language, Shona, and written consent was obtained from participants and parents/guardian before parasitology and blood samples were obtained. After collection of all samples, all participants were offered anti-helminthic treatment with the recommended dose of praziquantel (40 mg/kg of body weight).
Blood collection and isolation of PBMC
Depending on age of the participants up to 25 ml of venous blood was collected in heparinized tubes of which approximately 5 ml was used for serological assays as well as microscopic detection of malaria parasites. The remaining blood was used for the isolation of peripheral blood mononuclear cells (PBMC) through density gradient centrifugation using Lymphoprep (Axis-Shield, Cambridgeshire, UK). These PBMC were subsequently enumerated, cryo-preserved in 10% DMSO, 90% fetal calf serum and stored in liquid nitrogen in Zimbabwe prior to shipping to Edinburgh in dry shippers for assaying.
Thawing of cryo-preserved PBMC was performed by rotating cryovials in a 37°C water bath until a small crystal was remaining in the cell suspension. Cells were then slowly re-suspended in RPMI 1640 supplemented with 10% FCS, 2 mM L-glutamine and 100 U Penicillin/Streptomycin (all Lonza, Verviers, Belgium). Cells were washed twice with media, counted and viability assessed using trypan blue (Sigma-Aldrich, Dorsert, UK). The median viability of the PBMCs was 71.4% which is within the range of published values . In addition it was confirmed that including a viability marker did not change analysis of subsets. Afterwards, cells were washed with Dulbecco's-PBS (Lonza) and surface stained with the following antibodies: Qdot-605-conjugated anti-CD14 (clone TUK4, Invitrogen), FITC-conjugated anti-CD11c (clone Bu15, Invitrogen), APC-H7-conjugated anti-HLA-DR (clone L243, BD Biosciences), PE-Cy5. anti-CD123 (clone 9F5, BD Biosciences) and V450 BD Horizon-conjugated anti-CD86 (clone 2331, BD Biosciences), PE-conjugated anti-BDCA-2 (clone AC144, Miltenyi Biotec) and APC-conjugated anti-BDCA-4 (clone AD5-17F6, Miltenyi Biotec). Stained cells were acquired on a FACSCantoII (BD Biosciences) and analyzed using FlowJo software software (TreeStar, USA).
Serum antibody levels were measured by enzyme-linked immunosorbent assays (ELISA) following established protocols , . Whole worm homogenate (WWH) was obtained from the Theodor Bilharz Research Institute (Giza, Egypt). In short, microtiter plates were coated overnight at 4°C at 10 µg/ml. Serum samples were diluted at 1:20 for WWH-IgE and 1:100 for WWH-IgG and IgM and incubated for 2 hours at 37°C. Horse-radish peroxide conjugated antibodies were diluted 1:1000 for IgG, IgE and IgM and incubated for 1 hour at 37°C. ELISAs were developed using ABTS (Southern Biotech) and stopped after 15 minutes for IgG and IgM, and 30 minutes for IgE. Absorbance was read at 405 nm. Serum was available from 45 out of 61 individuals.
The proportions of DC cell subsets were square root arcsine transformed whereas expression levels of HLA-DR and CD86 were square root transformed to allow the use of parametric tests in subsequent analyses . To analyse which factors influence the proportion of DC subsets, a univariate analysis of variance using sex (male/female), host age groups (group 1: 5–9 years; group 2: 10–13 years; group 3: 14+ years) and infection status (un-infected = 0 mean egg count per 10 ml and infected >0 mean egg count per 10 ml; all variables categorical) as independent variables was performed. The three different age groups were selected to reflect epidemiological groupings (i.e. where infections are acquired, peak and decline) by age and infection intensity and to obtain comparable sample sizes between the groups. Post hoc tests between infected and un-infected individuals were conducted in each age group. In addition post hoc analysis was performed to determine differences in pDCs between the three age groups. To determine if the mDC populations showed an age profile consistent with those for protective immune responses as predicted by quantitative studies , correlation analyses between infection intensity (log10 (mean egg count+1) transformed) and proportions of mDCs was conducted after allowing for the effect of sex. The correlation coefficients were then tested for homogeneity using the Fisher's r-to-z transformation . Correlation analyses between WWH-specific IgG and DC cell subsets were performed after allowing for the effects of sex, age and infection intensity. All statistical tests were conducted using the software package SPSS v14 and p values were taken to be significant at p<0.05.
Epidemiology of study population
This study was designed to focus on the immune modulation in a population exposed to S. haematobium. The northeast part of Zimbabwe is endemic for S. haematobium with many regions having a moderate to high prevalence of S. haematobium, but low prevalence of S. mansoni and soil-transmitted helminths , . The overall prevalence in Chipinda was (39%). This is defined by the World Health Organisation (WHO) as a moderate transmission area . The overall prevalence of S. haematobium in the selected study population (N = 61) was 47.5% which is higher but not significantly different from (χ2 = 1.688, df = 1, p = 0.097) the village prevalence of 39% and still within the WHO definition of moderate transmission. The difference in infection prevalence between the youngest age group (35.3%) compared to more than 50% in the older age groups was not significant (χ2 = 0.806, df = 1, p = 0.185; details in Table 1). However, youngest individuals (5–9 years) who were infected carried high infection levels (Figure 1 and Table 1). Individuals aged 10–13 years still showed high infection levels, but with a higher prevalence than the first age group. In contrast, in the oldest age group (14–45 years), the prevalence remained high, but most individuals show lower infection intensities (Figure 1). The consequence of the infection profile is that individuals who are life long residents in the area and have never received treatment with anti-helminthic drugs (see selection criteria in Methods) differ in their cumulative histories of exposure despite carrying comparable infection intensities. This was supported by an analysis of the serum levels of adult worm specific (whole worm homogenate – WWH) antibody levels. As shown in Figure 2A and B levels of WWH-specific IgE and IgG, which are associated with history of infection and resistance to infection –, increase significantly with age. In contrast IgM against WWH as marker of current infection starts to decrease in the oldest age group (Figure 2C).
The study population was divided into three age groups: 5–9 years (N = 17), 10–13 years (N = 23) and 14+ years (N = 21). Mean infection intensities from at least two urine samples for each individual are given. The mean for each group is indicated.
Serum levels of WWH-specific IgE (A), IgG (B) and IgM (C) were analysed by ELISA. Obtained data are presented after diving into the three indicated age groups. Correlation coefficient b and significant values are indicated. The effect of sex has been statistically allowed for.
Characterization of mDCs and pDCs in total study population
To analyze mDCs and pDCs, PBMC were gated on CD14negHLA-DR+ cells (Figure 3A, B). Gated cells were stained with CD123 and CD11c to distinguish mDCs (CD11c+CD123neg/low) from pDCs (CD123hiCD11cneg; Figure 3C). To further verify specificity of pDC gated CD123hiCD11cneg were analysed for the expression of BDAC-2/CD303 and BDCA-4/CD304  and granularity of gated cells was analysed in an FSC/SSC plot (Figure 3D). Therefore other cell types such as basophiles or B cell potentially able to express CD123 are excluded from the analysis. mDC did not express either BDCA-2 or BDCA-4 (Figure 3E). Subsets were expressed as percentages of PBMC or as percentage of CD14negHLA-DR+ cells. Both subsets were subsequently analysed for the expression of CD86 (Figure 3D, E) and levels of HLA-DR within the two different DC populations as indicators of DC activation status and their ability to present antigen and co-stimulatory signals to T cells.
Live PBMCs (A) are gated on CD14negHLA-DR+ cells (B) which subsequently are analysed for the expression of CD11c and CD123 (C). CD123+CD11cneg (D) and CD123neg/lowCD11c+ (E) were subsequently analysed for the expression levels of BDCA-2/CD303 and BDCA-4/CD304, CD86 and HLA-DR. Presented plots are an example and derived from a 5 year old female individual.
When the study population was portioned by infection status (un-infected versus infected), neither mDCs (Figure 4A) nor pDCs (Figure 4B) showed a significant difference between both populations. Comparable results were obtained if DC subsets were expressed as percentage of CD14negHLA-DR+ (data not shown). Myeloid DCs showed a significant association with sex (Table 2), in which female had slightly higher proportions than males. This was then statistically accounted for in all subsequent analyses.
PBMC (N = 61) were analysed for proportions of (A) CD123neg/lowCD11c+ mDCs or (B) CD123hiCD11cneg pDCs (expressed as % of PBMC). Data of the total population were divided in un-infected (N = 32, white box) and infected individuals (N = 29, gray box). Data are presented as Whiskers boxplot (10–90 percentiles). Differences were analysed by univariate analysis after accounting for the effect of sex.
Age dependent pattern of mDCs and pDCs
The age in the total population ranged from 5–45 years. Age group significantly affected the proportions of pDCs (Table 2), with a significant increase between 5–9 year olds (age group 1) and 14+ year olds (age group 3; p = 0.033). In contrast for mDCs the relationship with age varied depending on infection status (significant interaction between age group and infection status; Table 2). Based on these results post hoc analysis of mDC proportions by infection status was performed after partitioning the study population into the three different age groups (details in Table 1). In the youngest age group (Figure 5A) infected people had significantly higher mDC percentages than un-infected people. Results were comparable if expressed as percentage of CD14negHLA-DR+ (Figure S1A). This pattern differed in the second age group, where infected individuals showed fewer mDCs than un-infected individuals (Figure 5B) a difference which was even more pronounced in the oldest age groups (Figure 5C). A comparable analysis could be made by correlating infection intensity (rather than infection status) to percentages of mDCs in the three different age groups. In the youngest age group both parameters were positively correlated (b = 0.707, p = 0.001), whereas in the second age group the correlation was instead negative (b = −0.441, p = 0.018). This negative correlation was more pronounced in the third group (b = −0.540, p = 0.007; Figure 6). A test for the homogeneity of the correlations coefficients showed a significant difference between age group one and two (z = 3.89, p = 0.0001) and between the first and the third age group (z = 4.17, p<0.0001).
The study population was divided into three age groups: 5–9 years (A), 10–13 years (B) and 14+ years (C). Proportions of mDCs were compared between un-infected (white box) and infected (gray box) individuals and expressed as % percentage of PBMC. Differences between un-infected and infected groups in the different age groups were analysed by univariate analysis after accounting for the effect of sex.
The study population was divided into three age groups: 5–9 years (left panel), 10–13 years (middle panel) and 14+ years (right panel). Proportions of mDCs expressed as % of PBMC are plotted against infection intensity (log10 (mean egg count+1) transformed). Correlation coefficient b and significant values of a correlation analysis after allowing for the effect of sex are indicated.
In contrast, to the clear picture in the case of mDCs, there was no significant difference in the proportion of pDCs (expressed either relative to live PBMC or CD14negHLA-DR+) between un-infected and infected people in any of the three age groups as shown in Figure 7, Figure S1B and Table 2.
The study population was divided into three age groups: 5–9 years (A), 10–13 years (B) and 14+ years (C). Proportions of mDCs were compared between un-infected (white box) and infected (gray box) individuals and expressed as percentage of PBMC (B). Differences between un-infected and infected groups in the different age groups were analysed by univariate analysis after accounting for the effect of sex.
Expression of CD86 and HLA-DR on mDCs and pDCs
Up-regulation of CD86 is hallmark of maturation and activation of DCs and their ability to provide co-stimulatory signals to T cells. HLA-DR expression can be used as an indicator both of DC activation status and their potential to activate antigen-specific T cells. Neither infection status nor the interaction between infection status and age group influenced expression of CD86 on mDC as determined by ANOVA (Table 2), which was confirmed by post hoc tests comparing CD86 expression between un-infected and infected people after partitioning into age group (Figure 8A). However HLA-DR expression was significantly influenced by an interaction of age group and infection status (Table 2). Post hoc test showed that HLA-DR expression differed between un-infected and infected individuals, in the youngest age group. In this age group HLA-DR was significantly lower on mDCs from infected people compared to un-infected (Figure 8B). In contrast no difference was observed in HLA-DR expression in people 10–13 years of age or in the oldest age group. Neither CD86 nor HLA-DR expression on pDCs were dependent on age group, infection status or the interaction of these variables (Table 2).
PBMC were gated on mDCs as indicated in Figure 2 and analysed for the expression of CD86 (A) and HLA-DR (B). Data partitioned into three age groups as indicated and compared between un-infected (white columns) and infected individuals (gray columns).
Interaction between mDCs and WWH-IgE
Adult worm specific IgG can be used as a marker development of resistance against infection –. WWH-specific IgG increases with age and in our population especially between the first and second age group (Figure 2B) and to the same time changes in mDC proportions between un-infected and infected individuals occurred. Therefore the interaction between mDC and WWH-specific IgG was analysed. As indicated in Table S1, WWH-specific IgG is influenced by sex, age and infection intensity, but addition also by proportions of mDC. mDC are directly correlated to WWH-specific IgG after allowing for the effects of sex, age and infection (Figure 9A). Although showing the same tendency pDCs and WWH-specific IgG were not significantly correlated (Figure 9B). This correlation was most obvious in the oldest age group (Table S2) and overall more significant in un-infected (protected) individuals. Correlation between mDC proportions and WWH-specific IgE and IgM were not significant.
Serum levels of WWH-specific IgG were measured by ELISA. The effects of sex, age and infection intensity was statistically allowed for and residuals of this analysis were plotted against proportions of mDCs (A) or pDCs (B) in percentage of PBMC. β-coefficient and p- values of a linear correlation are indicated.
Urogenital schistosomiasis caused by S. haematobium shows a characteristic age-infection profile with increasing infections intensities during early childhood, peaking usually between the age of 9–15 years and than slowly declining towards adulthood –. This study investigated whether the age-related changes in schistosome infection pattern impacted on the proportions and phenotype of DCs.
We provide evidence that indeed there is an age-related pattern in the proportions of mDCs, if infected and un-infected people were compared. The proportion of mDCs were found to be present in significantly higher proportions in infected individuals compared to un-infected individuals in the youngest age group carrying heaviest infection levels. This subsequently changes in older age groups, were older infected individuals had lower relative numbers of mDCs compared to un-infected people. The gradual change between the three age groups became more apparent when the correlation between infection intensity (rather than infection status) and mDCs proportions were considered. In this case both parameters were positively correlated in the youngest age group and became a negative correlation with increasing age. This pattern is consistent with that predicted by quantitative studies for protective immune responses and has already been demonstrated for antibody responses associated with protection to re/infection in human hookworm  and schistosome infections . Importantly levels of adult worm specific IgG are correlated with proportions of mDCs. This effect was more significant in the oldest age group, in which protective immunity becomes effective.
Our data confirm some of the major findings of a previous study of DC populations in human schistosomiasis conducted by Everts et al . Proportions of mDCs were lower in an age group 14–45 years (mean 22.6 years) which is comparable to the population studied by Everts et al (17–39 years). Since cellular immune responses might depend on many different parameters such as transmission dynamics or genetic background of the investigated study population the consistency in DC proportions in schistosome-exposed human between our study and that by Everts et al, by itself is an important finding. A major difference between the two studies is that we did not observe differences in pDC percentages in regards to infection status, although we had high variation in pDC levels in the oldest age group. In deed a slight tendency of lower pDC proportions (expressed as percentage of PBMC) was observed in this age group. Differences in co-infections may also account for the differences between our observations and those of Everts et al  in Gabon. While the population in Zimbabwe had no co-infection with other helminths, Plasmodium or HIV, that in Gabon had co-infections with microfilaria observed in 48% of the schistosome infected individuals which could affect the pDCs proportions.
Both our study and that of Everts et al  clearly indicate the difference in mDC populations between infected and un-infected adults. However, since infections in moderate to high transmission areas occur at relatively young age, we were interested in the dynamics of the DCs over time, which was not possible in Evert's study. We wanted to determine when if at all the difference between infected and un-infected people was established. Therefore, we analysed DC cell populations in younger age groups. Our study demonstrates that a lower frequency of mDCs in infected people can be already observed in 10–13 year olds. This is accordance with changes in other immunological parameters as clearly shown for schistosome-specific IgG, IgE and IgM. The importance of DC in TH2 induction has been recently shown . By altering cytokine responses DC could finally be involved in isotype switching and development of protective immunity. This is supported by our finding of the correlation between schisotosome-specific IgG and mDCs.
Changes with age have been also observed for serum cytokines in other study populations with moderate/high schistosome transmission , , . For instance, high levels of parasite-specific or serum levels of IL-10 have been reported already at this age in different populations ,  and might contribute to such changes. For example, DCs treated with the regulatory cytokine IL-10 inhibit T cell activation –. Earlier studies showed that T cell hypo-responsiveness can be already observed at early ages , . The role of mDCs in induction of this impairment remains to be further investigated.
Other reasons for reduced relative numbers of mDCs in infected individuals in older age groups remain to be determined, e.g. enhanced apoptosis, reduced bone marrow output or increased migration to inflamed tissues or lymphoid organs were proposed by Everts et al . Indeed enhanced migration in the context of TH2 priming DCs has been reported , . This was induced by eosinophil-derived neurotoxin; a molecule elevated in serum of S. haematobium infected people . Everts et al reported a more general impairment of DCs affecting both TH1 and TH2 cytokines as well as IL-10 in older individuals (17–39 years). The mechanisms involved are still unclear since despite this impairment of DC function, older individuals carrying schistosome infection can show enhanced immunopathology ,  indicating an effective immune response.
In contrast, our data show that in the younger individuals (5–9 years) mDCs proportions were higher in peripheral blood of infected individuals in comparison to un-infected. Potential reasons for this include enhanced bone marrow output or reduced migration of DCs in the context of blood-born antigens . Infection levels were highest in the youngest age group of our study population and young people might have more blood migrating schistosome stages affecting proportions of circulating mDCs. In contrast enhanced immune response including enhanced migration of mDCs might lead to increased killing of infiltrating schistosomula in older age groups.
Dendritic cell activation/maturation is characterised by up-regulation of cell-surface markers such as CD86 and MHC class II molecules. As previously reported by Everts et al , we observed no changes in expression of CD86 in infected people. However HLA-DR levels differed. In the youngest age group (5–9 years), where we found higher percentages of mDCs, expression levels of HLA-DR were lower in infected people. Reduced HLA-DR expression might be an indication of less matured and possibly fewer migrating cells, which subsequently could result in a less pronounced induction of T cell response in an early phase of exposure to schistosomes accompanied by a more regulatory environment due to Tregs and parasite-specific IL-10 , , . Analysis of Tregs from another study cohort from the same population revealed a comparable pattern as recently published by our group . In addition a direct comparison of mDCs and Tregs would need a larger sample size, since statistical power is lost by comparing the two parameters in the presence of other potentially confounding variables such as host age.
In contrast to the study by Everts et al, we did not observe a significant difference of HLA-DR levels in the older age groups. Again more significant changes of HLA-DR in these age groups might be due to cumulative effect of co-infections, not present in our study population.
Inhibition of up-regulation of activation markers such as CD86 has been suggested to be involved in induction of tolerogenic DCs . However we did not see changes in the activation marker CD86. There is need to analyse other markers, such as B7-H1, B7-H2, lg-like transcripts 3 and 4 which might play a role in the function of tolerogenic DCs –, particularly in younger age groups.
Functional studies, will be very informative and will clarify some of these areas, but were beyond the scope of this study.
In summary this study clearly shows there is an age-related pattern in the proportions mDCs in people exposed to S. haematobium infection as is typical for many already investigated humoral and cellular responses. Furthermore, in the case of mDCs, this pattern differs between schistosome infected and un-infected people and also follows the age profile with infection of protective responses, which is supported by the correlation between schistosome-specific IgG and proportions of mDCs.
The mechanism and consequences (such as alterations in the potential to induce different T cell phenotypes) behind the association between mDCs and infection remain to be determined. Understanding the nature and dynamics of immunological parameters in natural helminth infections and, in particular, changes of DCs involved in polarising the immune responses has an important impact on the development of vaccines against schistosomes.
Proportions of mDCs and pDCs expressed as percentage of CD14neg-HLA-DR+ cells. The study population was divided into three age groups: 5–9 years (A), 10–13 years (B) and 14+ years (C). Proportions of mDCs (A) or pDC (B) were compared between un-infected (white box) and infected (gray box) individuals and expressed as % percentage of CD14neg-HLA-DR+. Differences between un-infected and infected groups in the different age groups were analysed by univariate analysis after accounting for the effect of sex.
Interaction between WWH-IgE and mDC or pDC.
Correlation between WWH-IgE and mDC or pDC.
We would like to thank the residents and children of Chipinda village as well as the teachers from Chipinda primary and secondary school for their participation and generous and kind support of this study. We are also very grateful for the co-operation of the Ministry of Health and Child Welfare in Zimbabwe. We like to thank the Provincial Medical Director of Mashonaland East, the Environmental Health Workers, nursing staff at Chitate Clinics and Murehwa Hospital. We are grateful to the members of the National Institutes of Health in Harare and the Biochemistry Department at University of Zimbabwe for technical and logistical support.
Conceived and designed the experiments: NN OL NM TM FT FM. Performed the experiments: NN DL IP IYDC. Analyzed the data: NN DL OL. Wrote the paper: NN FM LJA CDB. Contributed to field work: LJA CDB. Contributed to final version of the manuscript: LJA CDB OL FT.
- 1. van der Werf MJ, de Vlas SJ, Brooker S, Looman CW, Nagelkerke NJ, et al. (2003) Quantification of clinical morbidity associated with schistosome infection in sub-Saharan Africa. Acta Trop 86: 125–139. doi: 10.1016/s0001-706x(03)00029-9
- 2. Fisher AC (1934) A study of schistosomiaisis in the Stanleyville district of Congo. Transactions of the Royal Society of Tropical Medicine and Hygiene 28: 277–306. doi: 10.1016/s0035-9203(34)90066-3
- 3. Anderson RM, May RM (1992) Macroparasites. Infectious diseases of humans: dynamics and control: Oxford Science.
- 4. Woolhouse MEJ, Etard JF, Dietz K, Ndhlovu PD, Chandiwana SK (1998) Heterogeneities in schistosome transmission dynamics and control. Parasitology 117: 475–482. doi: 10.1017/s003118209800331x
- 5. Clarke VD (1966) The influence of acquired resistance in the epidemiology of bilharziasis. Cent Afr J Med 12: 1–30.
- 6. Woolhouse MEJ, Taylor P, Matanhire D, Chandiwana SK (1991) Acquired immunity and epidemiology of Schistosoma haematobium. Nature 351: 757–759. doi: 10.1038/351757a0
- 7. Ndhlovu P, Cadman H, Vennervald BJ, Christensen NO, Chidimu N, et al. (1996) Age-related antibody profiles in Schistosoma haematobium in a rural community in Zimbabwe. Parasite Immunology 18: 181–191. doi: 10.1046/j.1365-3024.1996.d01-78.x
- 8. Mutapi F, Ndhlovu PD, Hagan P, Woolhouse ME (1997) A comparison of humoral responses to Schistosoma haematobium in areas with low and high levels of infection. Parasite Immunol 19: 255–263. doi: 10.1046/j.1365-3024.1997.d01-206.x
- 9. Webster M, LibrandaRamirez BDL, Aligui GD, Olveda RM, Ouma JH, et al. (1997) The influence of sex and age on antibody isotype responses to Schistosoma mansoni and Schistosoma japonicum in human populations in Kenya and the Philippines. Parasitology 114: 383–393. doi: 10.1017/s003118209600858x
- 10. Milner T, Reilly L, Nausch N, Midzi N, Mduluza T, et al. (2010) Circulating cytokine levels and antibody responses to human Schistosoma haematobium: IL-5 and IL-10 levels depend upon age and infection status. Parasite Immunology 32: 710–721. doi: 10.1111/j.1365-3024.2010.01235.x
- 11. Mutapi F, Winborn G, Midzi N, Taylor M, Mduluza T, et al. (2007) Cytokine responses to Schistosoma haematobium in a Zimbabwean population: contrasting profiles for IFN-gamma, IL-4, IL-5 and IL-10 with age. BMC Infect Dis 7: 139. doi: 10.1186/1471-2334-7-139
- 12. Nausch N, Midzi N, Mduluza T, Maizels RM, Mutapi F (2011) Regulatory and activated T cells in human Schistosoma haematobium infections. PLoS One 6: e16860. doi: 10.1371/journal.pone.0016860
- 13. Woolhouse MEJ (1992) A theoretical framework for the immunoepidemiology of helminth infection. Parasite Immunol 14: 563–578. doi: 10.1111/j.1365-3024.1992.tb00029.x
- 14. Turner JD, Faulkner H, Kamgno J, Cormont F, Van Snick J, et al. (2003) Th2 cytokines are associated with reduced worm burdens in a human intestinal helminth infection. J Infect Dis 188: 1768–1775. doi: 10.1086/379370
- 15. Quinnell R, Grafen A, Woolhouse M (1995) Changes in parasite aggregation with age: a discrete infection model. Parasitology 111: 635–644. doi: 10.1017/s003118200007712x
- 16. Mduluza T, Ndhlovu PD, Midzi N, Mary C, Paris CP, et al. (2001) T cell clones from Schistosoma haematobium infected and exposed individuals lacking distinct cytokine profiles for Th1/Th2 polarisation. Mem Inst Oswaldo Cruz 96 Suppl: 89–101. doi: 10.1590/s0074-02762001000900013
- 17. Joseph S, Jones FM, Kimani G, Mwatha JK, Kamau T, et al. (2004) Cytokine production in whole blood cultures from a fishing community in an area of high endemicity for Schistosoma mansoni in Uganda: the differential effect of parasite worm and egg antigens. Infect Immun 72: 728–734. doi: 10.1128/iai.72.2.728-734.2004
- 18. Watanabe K, Mwinzi PN, Black CL, Muok EM, Karanja DM, et al. (2007) T regulatory cell levels decrease in people infected with Schistosoma mansoni on effective treatment. Am J Trop Med Hyg 77: 676–682.
- 19. Capron A, Dombrowicz D, Capron M (1999) Regulation of the immune response in experimental and human schistosomiasis: the limits of an attractive paradigm. Microbes Infect 1: 485–490. doi: 10.1016/s1286-4579(99)80086-0
- 20. Dunne D, Mountford AP (2001) Resistance to infection in human and animal models. Schistosomiasis; Mahmoud AAF, editor. London: Imperial College Press.
- 21. Silveira AM, Gazzinelli G, Alves-Oliveira LF, Bethony J, Gazzinelli A, et al. (2004) Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-gamma and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures. Trans R Soc Trop Med Hyg 98: 514–519. doi: 10.1016/j.trstmh.2003.11.009
- 22. Medhat A, Shehata M, Bucci K, Mohamed S, Dief AD, et al. (1998) Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment. J Infect Dis 178: 512–519. doi: 10.1086/515630
- 23. Phythian-Adams AT, Cook PC, Lundie RJ, Jones LH, Smith KA, et al. (2010) CD11c depletion severely disrupts Th2 induction and development in vivo. J Exp Med 207: 2089–2096. doi: 10.1084/jem.20100734
- 24. Perona-Wright G, Jenkins SJ, MacDonald AS (2006) Dendritic cell activation and function in response to Schistosoma mansoni. Int J Parasitol 36: 711–721. doi: 10.1016/j.ijpara.2006.02.003
- 25. Yang D, Chen Q, Su SB, Zhang P, Kurosaka K, et al. (2008) Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2-MyD88 signal pathway in dendritic cells and enhances Th2 immune responses. J Exp Med 205: 79–90. doi: 10.1084/jem.20062027
- 26. MacDonald AS, Maizels RM (2008) Alarming dendritic cells for Th2 induction. J Exp Med 205: 13–17. doi: 10.1084/jem.20072665
- 27. Faveeuw C, Angeli V, Fontaine J, Maliszewski C, Capron A, et al. (2002) Antigen presentation by CD1d contributes to the amplification of Th2 responses to Schistosoma mansoni glycoconjugates in mice. J Immunol 169: 906–912.
- 28. van der Kleij D, Latz E, Brouwers JF, Kruize YC, Schmitz M, et al. (2002) A novel host-parasite lipid cross-talk. Schistosomal lyso-phosphatidylserine activates toll-like receptor 2 and affects immune polarization. J Biol Chem 277: 48122–48129. doi: 10.1074/jbc.m206941200
- 29. Aksoy E, Zouain CS, Vanhoutte F, Fontaine J, Pavelka N, et al. (2005) Double-stranded RNAs from the helminth parasite Schistosoma activate TLR3 in dendritic cells. J Biol Chem 280: 277–283. doi: 10.1074/jbc.m411223200
- 30. Vanhoutte F, Breuilh L, Fontaine J, Zouain CS, Mallevaey T, et al. (2007) Toll-like receptor (TLR)2 and TLR3 sensing is required for dendritic cell activation, but dispensable to control Schistosoma mansoni infection and pathology. Microbes Infect 9: 1606–1613. doi: 10.1016/j.micinf.2007.09.013
- 31. van der Kleij D, van den Biggelaar AH, Kruize YC, Retra K, Fillie Y, et al. (2004) Responses to Toll-like receptor ligands in children living in areas where schistosome infections are endemic. J Infect Dis 189: 1044–1051. doi: 10.1086/382089
- 32. Everts B, Adegnika AA, Kruize YC, Smits HH, Kremsner PG, et al. (2010) Functional impairment of human myeloid dendritic cells during Schistosoma haematobium infection. PLoS Negl Trop Dis 4: e667. doi: 10.1371/journal.pntd.0000667
- 33. Olweus J, BitMansour A, Warnke R, Thompson PA, Carballido J, et al. (1997) Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci U S A 94: 12551–12556. doi: 10.1073/pnas.94.23.12551
- 34. Robinson SP, Patterson S, English N, Davies D, Knight SC, et al. (1999) Human peripheral blood contains two distinct lineages of dendritic cells. Eur J Immunol 29: 2769–2778. doi: 10.1002/(sici)1521-4141(199909)29:09<2769::aid-immu2769>3.0.co;2-2
- 35. Colonna M, Trinchieri G, Liu YJ (2004) Plasmacytoid dendritic cells in immunity. Nat Immunol 5: 1219–1226. doi: 10.1038/ni1141
- 36. O'Doherty U, Peng M, Gezelter S, Swiggard WJ, Betjes M, et al. (1994) Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology 82: 487–493.
- 37. Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, et al. (2000) BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165: 6037–6046.
- 38. Cao W, Rosen DB, Ito T, Bover L, Bao M, et al. (2006) Plasmacytoid dendritic cell-specific receptor ILT7-Fc epsilonRI gamma inhibits Toll-like receptor-induced interferon production. J Exp Med 203: 1399–1405. doi: 10.1084/jem.20052454
- 39. Matta BM, Castellaneta A, Thomson AW (2010) Tolerogenic plasmacytoid DC. Eur J Immunol 40: 2667–2676. doi: 10.1002/eji.201040839
- 40. Gregori S (2011) Dendritic cells in networks of immunological tolerance. Tissue Antigens 77: 89–99. doi: 10.1111/j.1399-0039.2010.01615.x
- 41. Mutapi F, Rujeni N, Bourke C, Mitchell K, Appleby L, et al. (2011) Schistosoma haematobium treatment in 1–5 year old children: safety and efficacy of the antihelminthic drug praziquantel. PLoS Negl Trop Dis 5: e1143. doi: 10.1371/journal.pntd.0001143
- 42. Bethony JM, Cole RN, Guo X, Kamhawi S, Lightowlers MW, et al. (2011) Vaccines to combat the neglected tropical diseases. Immunol Rev 239: 237–270. doi: 10.1111/j.1600-065x.2010.00976.x
- 43. Smith MA, Clegg JA, Webbe G (1976) Cross-immunity to Schistosoma mansoni and S. haematobium in the hamster. Parasitology 73: 53–64. doi: 10.1017/s0031182000051325
- 44. Stevens WJ, Feldmeir H, Bridts CH, Daffalla AA (1983) IgG and IgE circulating immune complexes, total serum IgE and parasite related IgE in patients with mono- or mixed infection with Schistosoma mansoni and/or S. haematobium. Influence of therapy. Clin Exp Immunol 52: 144–152. doi: 10.1159/000234870
- 45. Geiger SM (2008) Immuno-epidemiology of Schistosoma mansoni infections in endemic populations co-infected with soil-transmitted helminths: present knowledge, challenges, and the need for further studies. Acta Trop 108: 118–123. doi: 10.1016/j.actatropica.2008.05.012
- 46. Chandiwana SK (1989) The problem and control of gastrointestinal helminthiases in Zimbabwe. Eur J Epidemiol 5: 507–515. doi: 10.1007/bf00140148
- 47. Ndhlovu P, Chimbari M, Ndamba J, Chandiwana SK (1992) 1992 National Schistosomiasis Survey. Blair Research Laboratory, Harare, Zimbabwe
- 48. Ndhlovu P, Chimbari M, Ndmba J, Chandiwana SK (1996) 1992 national schistosomiasis survey, Blair report for Zimbabwe. Blair Research Institute
- 49. Mutapi F, Hagan P, Ndhlovu P, Woolhouse MEJ (1998) Changes in specific anti-egg antibody levels following treatment with praziquantel for Schistosoma haematobium infection in children. Parasite Immunology 20: 595–600. doi: 10.1046/j.1365-3024.1998.00192.x
- 50. Mutapi F, Burchmore R, Foucher A, Harcus Y, Nicoll G, et al. (2005) Praziquantel treatment of people exposed to Schistosoma haematobium enhances serological recognition of defined parasite antigens. Journal of Infectious Diseases 192: 1108–1118. doi: 10.1086/432553
- 51. Mott KE (1983) A reusable polyamide filter for diagnosis of S. haematobium infection by urine filtration. Bull Soc Pathol Exot 76: 101–104.
- 52. Katz N, Chaves A, Pellegrino J (1972) A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Rev Inst MedTrop de Sao Paulo 14: 397–400.
- 53. World Health Organisation (1997) Revised Recommendation for the Selection and use of HIV antibody tests. Geneva. Available: http://www.who.int/diagnostics_laboratory/publications/en/Selection_HIV_tests.pdf Accessed 2012 Jan p.
- 54. Weinberg A, Zhang L, Brown D, Erice A, Polsky B, et al. (2000) Viability and functional activity of cryopreserved mononuclear cells. Clin Diagn Lab Immunol 7: 714–716. doi: 10.1128/cdli.7.4.714-716.2000
- 55. Rujeni N, Nausch N, Midzi N, Mduluza T, Taylor DW, et al. (2012) Schistosoma haematobium infection levels determine the effect of praziquantel treatment on anti-schistosome and anti-mite antibodies. Parasite Immunol doi: 10.1111/j.1365-3024.2012.01363.x
- 56. Mutapi F, Roddam A (2002) p-values for pathogens: statistical inference from infectious-disease data. Lancet Infectious Disease 2: 219–230. doi: 10.1016/s1473-3099(02)00240-2
- 57. Zar JH (1998) Biostatistical Analysis: Prentice Hall.
- 58. World Health Organization (2002) Prevention and control of schistosomiasis and soil-transmisted helminthiasis. Geneva: World Health Organisation. Available: http://www.who.int/wormcontrol/documents/joint_statements/en/ppc_unicef_finalreport.pdf Accessed 2011 Oct. p.
- 59. Hagan P, Blumenthal UJ, Dunne D, Simpson AJG, Wilkins AH (1991) Human IgE, IgG4 and resistance to reinfection with Schistosoma haematobium. Nature 349: 234–245. doi: 10.1038/349243a0
- 60. Mutapi F, Hagan P, Ndhlovu P, Woolhouse MEJ (1997) Comparison of humoral responses to Schistosoma haematobium in areas with high and low levels of infection. Parasite Immunology 19: 255–263. doi: 10.1046/j.1365-3024.1997.d01-206.x
- 61. Satti MZ, Lind P, J VB, Sulaiman SM, Daffalla AA, et al. (1996) Specific immunoglobulin measurements related to exposure and resistance to Schistosoma mansoni infection in Sudanese canal cleaners. Clinical Experimental Immunology 106: 45–54. doi: 10.1046/j.1365-2249.1996.d01-810.x
- 62. Dzionek A, Inagaki Y, Okawa K, Nagafune J, Rock J, et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum Immunol 63: 1133–1148. doi: 10.1016/s0198-8859(02)00752-8
- 63. Quinnell RJ, Bethony J, Pritchard DI (2004) The immunoepidemiology of human hookworm infection. Parasite Immunol 26: 443–454. doi: 10.1111/j.0141-9838.2004.00727.x
- 64. Mutapi F, Mduluza T, Gomez-Escobar N, Gregory WF, Fernandez C, et al. (2006) Immuno-epidemiology of human Schistosoma haematobium infection: preferential IgG3 antibody responsiveness to a recombinant antigen dependent on age and parasite burden. BMC Infect Dis 6: 96.
- 65. Lyke KE, Dabo A, Sangare L, Arama C, Daou M, et al. (2006) Effects of concomitant Schistosoma haematobium infection on the serum cytokine levels elicited by acute Plasmodium falciparum malaria infection in Malian children. Infect Immun 74: 5718–5724. doi: 10.1128/iai.01822-05
- 66. Steinbrink K, Wolfl M, Jonuleit H, Knop J, Enk AH (1997) Induction of tolerance by IL-10-treated dendritic cells. J Immunol 159: 4772–4780.
- 67. Steinbrink K, Graulich E, Kubsch S, Knop J, Enk AH (2002) CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated human dendritic cells display antigen-specific suppressor activity. Blood 99: 2468–2476. doi: 10.1182/blood.v99.7.2468
- 68. Zheng Z, Narita M, Takahashi M, Liu A, Furukawa T, et al. (2004) Induction of T cell anergy by the treatment with IL-10-treated dendritic cells. Comp Immunol Microbiol Infect Dis 27: 93–103. doi: 10.1016/s0147-9571(03)00054-7
- 69. Torres-Aguilar H, Aguilar-Ruiz SR, Gonzalez-Perez G, Munguia R, Bajana S, et al. (2010) Tolerogenic dendritic cells generated with different immunosuppressive cytokines induce antigen-specific anergy and regulatory properties in memory CD4+ T cells. J Immunol 184: 1765–1775. doi: 10.4049/jimmunol.0902133
- 70. King CL, Medhat A, Malhotra I, Nafeh M, Helmy A, et al. (1996) Cytokine control of parasite-specific anergy in human urinary schistosomiasis. IL-10 modulates lymphocyte reactivity. J Immunol 156: 4715–4721.
- 71. Grogan JL, Kremsner PG, Deelder AM, Yazdanbakhsh M (1998) Antigen-specific proliferation and interferon-gamma and interleukin-5 production are down-regulated during Schistosoma haematobium infection. J Infect Dis 177: 1433–1437. doi: 10.1086/517832
- 72. Yang D, Rosenberg HF, Chen Q, Dyer KD, Kurosaka K, et al. (2003) Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells. Blood 102: 3396–3403. doi: 10.1182/blood-2003-01-0151
- 73. Tischendorf FW, Brattig NW, Burchard GD, Kubica T, Kreuzpaintner G, et al. (1999) Eosinophils, eosinophil cationic protein and eosinophil-derived neurotoxin in serum and urine of patients with onchocerciasis coinfected with intestinal nematodes and in urinary schistosomiasis. Acta Trop 72: 157–173. doi: 10.1016/s0001-706x(98)00088-6
- 74. Booth M, Vennervald BJ, Kabatereine NB, Kazibwe F, Ouma JH, et al. (2004) Hepatosplenic morbidity in two neighbouring communities in Uganda with high levels of Schistosoma mansoni infection but very different durations of residence. Trans R Soc Trop Med Hyg 98: 125–136. doi: 10.1016/s0035-9203(03)00018-x
- 75. Mohamed-Ali Q, Elwali NE, Abdelhameed AA, Mergani A, Rahoud S, et al. (1999) Susceptibility to periportal (Symmers) fibrosis in human schistosoma mansoni infections: evidence that intensity and duration of infection, gender, and inherited factors are critical in disease progression. J Infect Dis 180: 1298–1306. doi: 10.1086/314999
- 76. Balazs M, Martin F, Zhou T, Kearney J (2002) Blood dendritic cells interact with splenic marginal zone B cells to initiate T-independent immune responses. Immunity 17: 341–352. doi: 10.1016/s1074-7613(02)00389-8
- 77. Tuettenberg A, Huter E, Hubo M, Horn J, Knop J, et al. (2009) The role of ICOS in directing T cell responses: ICOS-dependent induction of T cell anergy by tolerogenic dendritic cells. J Immunol 182: 3349–3356. doi: 10.4049/jimmunol.0802733
- 78. Cella M, Dohring C, Samaridis J, Dessing M, Brockhaus M, et al. (1997) A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing. J Exp Med 185: 1743–1751. doi: 10.1084/jem.185.10.1743
- 79. Colonna M, Samaridis J, Cella M, Angman L, Allen RL, et al. (1998) Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules. J Immunol 160: 3096–3100.
- 80. Selenko-Gebauer N, Majdic O, Szekeres A, Hofler G, Guthann E, et al. (2003) B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy. J Immunol 170: 3637–3644.
- 81. Sumpter TL, Thomson AW (2011) The STATus of PD-L1 (B7-H1) on tolerogenic APCs. Eur J Immunol 41: 286–290. doi: 10.1002/eji.201041353