Many neglected tropical infectious diseases affecting humans are transmitted by arthropods such as mosquitoes and ticks. New mode-of-action chemistries are urgently sought to enhance vector management practices in countries where arthropod-borne diseases are endemic, especially where vector populations have acquired widespread resistance to insecticides.
We describe a “genome-to-lead” approach for insecticide discovery that incorporates the first reported chemical screen of a G protein-coupled receptor (GPCR) mined from a mosquito genome. A combination of molecular and pharmacological studies was used to functionally characterize two dopamine receptors (AaDOP1 and AaDOP2) from the yellow fever mosquito, Aedes aegypti. Sequence analyses indicated that these receptors are orthologous to arthropod D1-like (Gαs-coupled) receptors, but share less than 55% amino acid identity in conserved domains with mammalian dopamine receptors. Heterologous expression of AaDOP1 and AaDOP2 in HEK293 cells revealed dose-dependent responses to dopamine (EC50: AaDOP1 = 3.1±1.1 nM; AaDOP2 = 240±16 nM). Interestingly, only AaDOP1 exhibited sensitivity to epinephrine (EC50 = 5.8±1.5 nM) and norepinephrine (EC50 = 760±180 nM), while neither receptor was activated by other biogenic amines tested. Differential responses were observed between these receptors regarding their sensitivity to dopamine agonists and antagonists, level of maximal stimulation, and constitutive activity. Subsequently, a chemical library screen was implemented to discover lead chemistries active at AaDOP2. Fifty-one compounds were identified as “hits,” and follow-up validation assays confirmed the antagonistic effect of selected compounds at AaDOP2. In vitro comparison studies between AaDOP2 and the human D1 dopamine receptor (hD1) revealed markedly different pharmacological profiles and identified amitriptyline and doxepin as AaDOP2-selective compounds. In subsequent Ae. aegypti larval bioassays, significant mortality was observed for amitriptyline (93%) and doxepin (72%), confirming these chemistries as “leads” for insecticide discovery.
This research provides a “proof-of-concept” for a novel approach toward insecticide discovery, in which genome sequence data are utilized for functional characterization and chemical compound screening of GPCRs. We provide a pipeline useful for future prioritization, pharmacological characterization, and expanded chemical screening of additional GPCRs in disease-vector arthropods. The differential molecular and pharmacological properties of the mosquito dopamine receptors highlight the potential for the identification of target-specific chemistries for vector-borne disease management, and we report the first study to identify dopamine receptor antagonists with in vivo toxicity toward mosquitoes.
Mosquitoes and other arthropods transmit important disease-causing agents affecting human health worldwide. There is an urgent need to discover new chemistries to control these pests in order to reduce or eliminate arthropod-borne diseases. We describe an approach to identify and evaluate potential insecticide targets using publicly available genome (DNA) sequence information for arthropod disease vectors. We demonstrate the utility of this approach by first determining the molecular and pharmacological properties of two different dopamine (neurotransmitter) receptors of the yellow fever- and dengue-transmitting mosquito, Aedes aegypti. Next, we tested 1,280 different chemistries for their ability to interact with one of these dopamine receptors in a chemical screen, and 51 “hit” compounds were identified. Finally, we show that two of these chemistries, amitriptyline and doxepin, are selective for the mosquito over the human dopamine receptor and that both chemistries caused significant mortality in mosquito larvae 24 hours after exposure, identifying them as possible “leads” for insecticide development. Our methodology is adaptable for chemical screening of related targets in mosquitoes and other arthropod vectors of disease. This research demonstrates the potential of target-specific approaches that could complement traditional phenotypic screening, and ultimately may accelerate discovery of new mode-of-action insecticides for vector control.
Citation: Meyer JM, Ejendal KFK, Avramova LV, Garland-Kuntz EE, Giraldo-Calderón GI, et al. (2012) A “Genome-to-Lead” Approach for Insecticide Discovery: Pharmacological Characterization and Screening of Aedes aegypti D1-like Dopamine Receptors. PLoS Negl Trop Dis 6(1): e1478. doi:10.1371/journal.pntd.0001478
Editor: Rhoel Ramos Dinglasan, Johns Hopkins Bloomberg School of Public Health, United States of America
Received: July 15, 2011; Accepted: November 29, 2011; Published: January 24, 2012
Copyright: © 2012 Meyer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was supported by a Purdue University, Discovery Park Seed Grant (#203606) and a Department of Defense/TATRC sub-award (#201596-PU) to C.A.H. and V.J.W. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Mosquitoes (Class Insecta; Order Diptera; Family Culicidae) vector multiple neglected tropical diseases (NTDs) affecting human health, including malaria, yellow-fever, dengue and filariasis. Historically, insecticides employed against arthropod disease vectors have reduced the impact of NTDs, but unfortunately, continued disease control is threatened by the widespread development of vector populations that are resistant to insecticidal chemistries . This issue is further complicated by the fact that there has not been a new public health insecticide produced for vector-borne disease control for over 30 years . Recently, philanthropic investment has focused attention toward the development of new drugs to control NTDs in the human population . It is widely recognized that an arsenal of new vector control solutions are required in order to meet this and other public health goals regarding NTDs. Thus, the research community should aggressively pursue the discovery of new mode-of-action chemistries for mosquito control through both traditional phenotypic screening and target-based approaches.
Novel insecticide targets potentially exist among the arthropod G protein-coupled receptors (GPCRs). These proteins comprise a large family of membrane-bound molecules that mediate critical biological processes such as neurotransmission, vision, and hormonal regulation, among others , . GPCRs are extensively targeted for drug development in humans - approximately 40% of prescription pharmaceuticals interact with these receptors  - and more recently, Gamo et al.  reported multiple GPCR-interacting chemistries as promising anti-malarial leads. Also, the mode-of-action of amitraz, a chemistry registered for tick and insect control, is presumed to have partial agonistic activity at an octopamine sensitive GPCR . More than 100 different GPCRs have been identified in the genomes of multiple insect species, including malaria- and yellow fever-transmitting mosquitoes , . These studies have provided a basis for the functional characterization of GPCRs and their prioritization as potential subjects for insecticide development.
The biogenic amine-binding GPCRs (rhodopsin-like) are integral components of the central and peripheral nervous systems of eukaryotes and include receptors that bind the neurotransmitters dopamine, histamine, octopamine, serotonin, tyramine, and acetylcholine . The dopamine receptors are classified as either D1- or D2-like  based on their differential functional roles. Ligand binding to the D1-like dopamine receptors causes Gαs-mediated stimulation of adenylyl cyclase (AC) production of cAMP. A reciprocal effect is observed following agonist activation of D2-like dopamine receptors, whereby cAMP production by AC is inhibited via Gαi/o proteins. Dopamine and its receptors are essential for complex behavioral mechanisms in arthropods such as locomotion , , , arousal , and olfactory learning , .
The importance of dopaminergic-related functions has stimulated research to understand these processes in mosquitoes. Dopamine and serotonin have been tied to salivary gland functioning of vectors ,  and may have an impact on pathogen acquisition and transmission during blood feeding. Andersen et al.  reported that increased levels of dopamine were detected in Aedes aegypti following a blood meal that were implicated in ovarian or egg development, and in newly-emerged adults, presumably as part of the sclerotization process. Much attention has been given to the role of dopamine in the melanization pathway of mosquitoes and other insects, as well as the effect of dopamine on development, pigmentation, reproduction, immune responses to parasites, wound healing, and Wolbachia infection , , , , , . In the mosquito Culex pipiens, dose-dependent increases in cAMP were detected following treatment with dopamine and octopamine in homogenized head tissues, suggesting the presence of Gαs-coupled receptors that are responsive to these biogenic amines . Putative D1-like and D2-like dopamine receptors have been identified in the genomes of the mosquitoes Ae. aegypti  and Anopheles gambiae , but research investigating their pharmacological properties is lacking. These genomic sequences provide a logical starting point to functionally characterize the receptors, which is needed to improve our comprehension of dopaminergic processes in mosquitoes. Moreover, due to their presumed significance in mosquito neurobiology, these dopamine receptors are attractive candidates to explore as new targets for chemical control.
We present the results of a “proof-of-concept” study involving a “genome-to-lead” approach for developing new mode-of-action insecticides for arthropod disease vectors (Figure 1A). Our research strategy involves (i) exploitation of an arthropod genome sequence for novel target identification, (ii) molecular, biochemical and pharmacological target validation, (iii) chemical library screening, and (iv) confirmation of hits and identification of candidate “leads” using secondary in vitro assays and mosquito in vivo assays. Toward these objectives, two dopamine receptors (AaDOP1 and AaDOP2) were identified in the genome of the yellow-fever mosquito, Ae. aegypti, and characterized using molecular and pharmacological methods. Subsequently, we conducted a chemical library screen in which multiple lead antagonistic chemistries of the AaDOP2 receptor were identified. Finally, we employed a “hit-to-lead” approach (Figure 1B), wherein screen “hits” were confirmed in secondary in vitro assays and two “lead” chemistries were identified using in vivo assays that confirmed their toxicity to mosquito larvae. These results serve as an entry point for expanded chemical library screening of mosquito dopamine receptors and subsequent structure-activity relationship- and further “hit-to-lead”-studies to discover candidate compounds that will enter the registration phase of product development (Figure 1A). Our pipeline will expedite the exploration of GPCRs as potential targets for chemical control in mosquitoes and other important arthropod disease vectors for which sufficient genome sequence data is available.
A: The illustration shows critical steps involved with the “genome-to-lead” (described in this manuscript) and “lead-to-product” phases. Abbreviations: (EPA) Environmental Protection Agency; (FDA) Food and Drug Administration; (SAR) structure-activity relationship study. The intended administration route of a particular chemistry dictates the federal agency that will receive the registration package; B: Expanded details of the “hit-to-lead” phase including those pursued in this study.
Materials and Methods
The gene sequences for the putative dopamine receptors AaegGPRdop1 (AAEL003920) and AaegGPRdop2 (AAEL005834) (referred to hereafter as Aadop1 and Aadop2, respectively) in Ae. aegypti  were downloaded from VectorBase (http://www.vectorbase.org/index.php) . Sequences of the D1-like dopamine receptors in Drosophila melanogaster were used to identify and compare conserved structural features , .
Gene expression analyses for each receptor were conducted using RNA extracted from the eggs, larvae, pupae, and adult male and female mosquitoes from the Liverpool strain of Ae. aegypti . Total RNA was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA) and then treated with RNase-Free DNase (QIAGEN, Valencia, CA). The SuperScript One-Step RT-PCR kit (Invitrogen, Carlsbad, CA) was used to amplify receptor mRNA from approximately 150 ng total RNA per reaction using the primers and experimental conditions provided in Table S1. RT-PCR amplification products were electrophoresed and compared by size to the DNA HyperLadder I (Bioline USA Inc., Randolph, MA). Products were cut from the gel and isolated with the Qiagen Gel Extraction Kit (Qiagen Valencia, CA). The cloning procedure was performed using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions. DNA sequencing was conducted at the Purdue University Genomics Core Facility. The resultant DNA sequences were used to predict full-length coding regions that were manually annotated using Artemis software (version 9) .
A neighbor-joining sequence analysis was performed using the deduced amino acid sequences representing the mosquito dopamine receptor proteins (referred to hereafter as AaDOP1 and AaDOP2), additional representative biogenic amine receptors from the insects D. melanogaster and A. mellifera, and the human D1- and D2-like dopamine receptors. ClustalW 1.83  was used for sequence alignments prior to tree construction in PAUP 4.0b4a . The bootstrap method (100 replicates) was used to provide branch support. Alignments of amino acid sequences for determination of conserved motifs were conducted using Multalin software . Conserved amino acid residues and additional protein features were predicted as described by Meyer et al. .
Functional characterization of AaDOP1 and AaDOP2 was conducted by heterologous expression in HEK293 cells (ATCC, Manassas, VA) . Expression constructs were produced by synthesis (GenScript, Piscataway, NJ) and included the partial Kozak transcriptional recognition sequence “CACC” added directly upstream of the transcription initiation codon for each gene. Constructs were cloned into pUC57 and then subcloned into the expression vector pcDNA3.1+ (Invitrogen, Carlsbad, CA) by GenScript (Piscataway, NJ). Stable cell lines co-expressing either AaDOP1 or AaDOP2 with a CRELuc reporter construct were developed to permit pharmacological studies in a 384-well format , . Briefly, cells already stably expressing the CRELuc reporter construct were transfected in a 10 cm dish with 15 µl Lipofectamine2000 and 3 µg of pcDNA3.1+/Aadop1 or pcDNA3.1+/Aadop2. Clones were maintained as described for the wild-type HEK293 cells  with the addition of 2 µg/ml puromycin and 300 µg/ml Geneticin (Sigma-Aldrich, St. Louis, MO).
For initial functional analysis, the receptors were transiently expressed in HEK293 cells  and analyzed using a competitive binding assay to measure levels of cAMP accumulation . Dose-response curves were generated using cells stably expressing the receptors , . The compounds used for pharmacological characterization included dopamine hydrochloride, histamine dihydrochloride, 5-hydroxytryptamine hydrochloride (serotonin), (±)-octopamine hydrochloride, tyramine hydrochloride (Sigma-Aldrich, St. Louis, MO), (−)-epinephrine bitartrate, and L (−)-norepinephrine bitartrate (Research Biochemical International, Natick, MA). The synthetic dopamine receptor ligands tested included SKF38393 and SKF81297 (Tocris, Ellisville, MO), SCH23390 (Tocris, Ellisville, MO), and dihydrexidine (DHX) (a gift from D. Nichols, Purdue University). Data was collected from a minimum of three independent replicate experiments with each sample measured in triplicate. Statistical analysis of data was conducted with GraphPad Prism 5 software (GraphPad Software Inc., San Diego, CA).
Screening of AaDOP2 against the LOPAC1280 library
To identify novel AaDOP2 receptor antagonists, the Library of Pharmacologically Active Compounds (LOPAC1280) was screened at the Integrated Screening Technologies Laboratory, Discovery Park, Purdue University, using HEK-CRELuc-Aadop2 cells. These cells were cultured as described above, expanded, and cryo-preserved, to produce a uniform cell population. Briefly, cells (~2.5×107) were harvested by non-enzymatic dissociation [0.5 mM EDTA in Ca2+Mg2+free-phosphate buffered saline (CMF-PBS)] resuspended in cell culture media, and pelleted by centrifugation for 5 min at 100× G. The pellet was resuspended in freezing media (Opti-MEM supplemented with 10% DMSO and 20% FBS) to a concentration of 5×106/ml, frozen step-wise, and held in liquid N2 until use. Cells were rapidly thawed, diluted in Opti-MEM, and 20 µl containing 25,000 cells were plated per well in 384-well plates (Nunc, Fisher Scientific, Pittsburgh, PA) using a BiomekFX liquid handling station (Beckman-Coulter, Brea, CA). The plates were incubated overnight in a humidified incubator at 37°C and 5% CO2.
Prior to screen initiation, a “checkerboard” analysis was conducted that included a minimum (300 nM dopamine in combination with 10 µM SCH23390) and maximum (300 nM dopamine) stimulatory condition. The data obtained were analyzed to calculate the Z-factor  using a modified equation that accounts for the number of replicates (NIH website: http://assay.nih.gov/assay/index.php/Section2:Plate_Uniformity_and_Signal_Variability_Assessment).
All compounds were diluted to appropriate concentrations and suspended in assay buffer (Opti-MEM supplemented with 0.02% ascorbic acid) using a BiomekFX 96-tip head. All LOPAC1280 compounds were screened in quadruplicate at a concentration of 10 µM, including duplicate samples on two separate assay plates in different quadrants to control for plate and automation effects. Each plate contained a dopamine response curve (14 nM–30 µM) and antagonist control wells (10 µM SCH23390 in combination with 300 nM dopamine). Following compound addition, dopamine was added to each test well at a final concentration of 300 nM, and cells were incubated for 2 hr at 37°C in a humidified incubator. The plates were then equilibrated at 25°C prior to the addition of Steadylite plus luminescence reagent (PerkinElmer, Shelton, CT). Plates were incubated on a shaker at 300 rpm for 5 min, and the luminescence signal was measured using a DTX880 multimode reader (Beckman Coulter, Brea, CA) with a 1 sec integration time.
Raw screen data were processed as follows: the average background luminescence (cells in the absence of dopamine or LOPAC1280 compound) was subtracted from the raw data. Values for the positive receptor activation control (300 nM dopamine) were averaged within each assay plate and used to establish a 100% dopamine receptor stimulation level. Similarly, the average response to SCH23390 was calculated within each assay plate to establish a baseline inhibition for antagonist chemistries. The average percent compound effect was calculated for each LOPAC chemistry in comparison to the SCH23390 antagonist control. The minimum criterion for selection of an antagonist “hit” was established as the percent inhibition equivalent to that determined for SCH23390+3 standard deviations.
Confirmation and secondary in vitro assays.
Subsequent validation assays using both the AaDOP2 and the human D1 dopamine receptor (hD1)  were conducted for select identified “hit” chemistries using a competitive binding cAMP accumulation assay. In addition to SCH23390, these included amitriptyline hydrochloride, doxepin hydrochloride, niclosamide, clozapine, (+)-butaclamol hydrochloride, cis-(Z)-flupenthixol dihydrochloride, resveratrol, mianserin hydrochloride (Sigma, St. Louis, MO), piceatannol and methiothepin maleate (Tocris, Ellisville, MO). The drugs were suspended from dimethyl sulfoxide (DMSO) stocks in Hanks Balanced Salt Solution (HBSS) (HyClone, Logan, UT) with with 0.1% fatty acid free bovine serum albumin (BSA) and 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonicacid (HEPES), and serial dilutions were prepared using a Precision 2000 automated pipetting system (BioTek, Winooski, VT). The cAMP accumulation assay was carried out as previously described ,  with minor modifications to permit processing of a larger number of samples in a semi-automated fashion. Briefly, AaDOP2- or hD1-expressing cells were harvested using Hank's based non-enzymatic cell dissociation reagent (Invitrogen, Carlsbad, CA), resuspended in Dulbecco's modified eagle medium (DMEM) (Invitrogen, Carlsbad, CA), centrifuged 5 min at 100× G, and resuspended in HBSS supplemented with 0.1% BSA and 20 mM HEPES. Cells were seeded (50,000 cells in 40 µl) in clear 96-well plates and incubated at 37°C with 5% CO2 for 1 hr. The cAMP accumulation assay was carried out in HBSS supplemented with final concentrations of 0.1% BSA, 20 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), and 0.02% ascorbic acid in a final volume of 50 µl. The selected compounds were added to the wells in duplicate, followed by addition of dopamine (final concentration 3 µM for AaDOP2 and 100 nM for hD1). Plates were incubated at room temperature for 1 hr, and the assay was terminated by addition of 25 µl of 9% ice-cold trichloroacetic acid (TCA). Cell lysates were incubated on ice for at least 1 hr prior to quantifying cAMP accumulation as previously described , .
In vivo Ae. aegypti bioassays
Single dose-point and dose response in vivo mosquito bioassays were used to assess the toxicity of selected AaDOP2 receptor antagonists identified in the chemical screen. Larvae of Ae. aegypti (Liverpool strain) were reared under standard laboratory conditions on a 12 hr day/night cycle at 75% RH and 28°C, and bioassays were conducted at room temperature (22–24°C). Larvae were transferred from standard rearing trays into six-well tissue culture plates (Corning, Inc. Corning, NY) using a small plastic pipette. Ten L4-stage larvae were included per well, each containing five ml of de-ionized water and the assigned drug concentration. Controls were conducted similarly but lacked a drug treatment. Bioassays employed a double-blind experimental design, and percent mortality was scored 24 hr following administration of drugs. Single dose-point assays were conducted using 400 µM drug and included three biological replicates each consisting of 50–100 larvae. Dose-response assays were conducted using five doses (400, 200, 100, 50, and 25 µM) of the compounds suspended in water, with water alone as a control. Five technical replicates, each including 10 larvae, were performed per dose, and the assay was repeated three times. Statistical analyses included one sample t-tests (single-point assays) and determination of the LC50 and LC90 values (dose-response assays) conducted with GraphPad Prism 5 software (GraphPad Software Inc., San Diego, CA).
mRNA transcripts for Aadop1 and Aadop2 were detected by RT-PCR in eggs, larvae, pupae, and adult male and female Ae. aegypti (Figure S1). DNA sequencing of RT-PCR products confirmed the splice junctions at each intron/exon boundary for both receptor genes. Using a combination of evidence from our RT-PCR data, the genome sequence, and related sequences in D. melanogaster, we predicted the gene structure and complete coding regions of Aadop1 (Genbank accession: JN043502) and Aadop2 (Genbank accession: JN043503) (Figure S2). A neighbor-joining sequence analysis was conducted to assess the relationships of AaDOP1 and AaDOP2 with other representative biogenic amine receptors (Figure 2). AaDOP1 was included in a clade (bootstrap = 100) containing the presumably orthologous D1-like dopamine receptors D-Dop1 of D. melanogaster , , DOP1 of A. mellifera , and Isdop1 of I. scapularis , . AaDOP2 clustered with two presumably orthologous insect D1-like dopamine receptors (INDRs) , DopR99B (DAMB) of D. melanogaster ,  and DOP2 of A. mellifera , as well as Isdop2 of I. scapularis . The INDR-like and Isdop2 sequences were also joined together in a larger clade (bootstrap = 76) containing the octopamine receptors OAMB of D. melanogaster  and OCT1  of A. mellifera, consistent with Mustard et al. . The human D1-like dopamine receptors formed a separate clade (bootstrap = 100) distinct from the arthropod dopamine receptors.
The deduced amino acid sequences for the mosquito dopamine receptors AaDOP1 and AaDOP2 and additional receptors for dopamine, muscarinic acetylcholine, octopamine, serotonin, and tyramine from Drosophila melanogaster and Apis mellifera, as well as the human D1-like and D2-like dopamine receptors were aligned for use in the analysis. Bootstrap values (100 replicates) are indicated with numbers at supported branches. The outgroup is a D. melanogaster diuretic hormone receptor, a Class B GPCR. Abbreviations: Aa = Ae. aegypti; Is = I. scapularis; Dm = D. melanogaster; Am = A. mellifera; Hs = H. sapiens. Sequences: Isdop1, D1-like dopamine receptor (ISCW001496); Isdop2, D1-like dopamine receptor (ISCW008775); DmD-Dop1, D1-like dopamine receptor (P41596); DmDAMB, D1-like dopamine receptor (DopR99B/DAMB: AAC47161), DmDD2R, D2-like dopamine receptor (DD2R-606: AAN15955); DmDih, diuretic hormone 44 receptor 1 (NP_610960.1); DmmAChR, muscarinic acetylcholine receptor (AAA28676); DmOAMB, octopamine receptor in mushroom bodies, isoform A (NP_732541); DM5HT1A, serotonin receptor 1A, isoform A (NP_476802); DmTyr, tyramine receptor (CG7431: NP_650652); AmDOP1, D1-like dopamine receptor (dopamine receptor, D1, NP_001011595); AmDOP2, D1-like dopamine receptor (dopamine receptor 2, NP_001011567), AmDOP3, D2-like dopamine receptor (AmDOP3, NP_001014983); AmmAChR, muscarinic acetylcholine receptor (XP_395760); AmOA1, octopamine receptor (oar, NP_001011565); Am5HT1A, serotonin receptor (5ht-1, NP_001164579); AmTyr, tyramine receptor (XP_394231); HsD1, D1-like dopamine receptor (D(1A), NP_000785); HsD2,D2-like dopamine receptor (D(2), NP_000786); HsD3, D2-like dopamine receptor (D(3), NP_000787); HsD4, D2-like dopamine receptor (D(4), NP_000788); HsD5, D1-like dopamine receptor (D(1B)/D5, NP_000789).
The deduced amino acid sequences of AaDOP1 and AaDOP2 were analyzed to identify conserved structural features typically associated with biogenic amine-binding GPCRs (Table S2), as well as unique regions that could be potentially exploited for development of mosquito-specific chemistries. Conserved features included sites predicted for ligand binding, protein stability, and G protein-coupling, and residues with potential for post-translational modification were identified. Alignments of the full-length AaDOP1 and AaDOP2 amino acid sequences (Figure 3) indicated that these sequences were divergent in the presumed N- and C-termini and the intracellular and extracellular loops, and the TM domains were moderately conserved (47% amino acid identity). A substantial difference was observed in the composition and relative size of the third intracellular loop that was much larger in AaDOP2 (115 amino acids) than in AaDOP1 (62 amino acids). Only a modest level of similarity was observed between the mosquito and human D1-like dopamine receptors, which shared between 47–54% amino acid identities among the TM domains, which typically represent the most conserved regions of GPCRs (Table S3). Moreover, comparison of the predicted TM domains from multiple invertebrate and vertebrate D1-like dopamine receptors showed that only 34% (58/172) of the amino acids were shared among all species included in the alignment (Figure S3). The highest level of sequence similarity to the TM domains of AaDOP1 and AaDOP2 was found in their predicted D. melanogaster orthologs, D-Dop1 (88% identity) (Table S3) and DopR99B (97% identity), respectively.
Highlighted areas designate residues with shared biochemical characteristics, as designated by the ClustalW  output, where black shading = identical residues; dark shading = strongly similar residues; light shading = weakly similar residues. Also noted are the residues composing the N- and C-termini and the transmembrane (TM) domains I–VII.
Heterologous expression and pharmacological characterization
To study the function of the putative dopamine receptors AaDOP1 and AaDOP2, each receptor was expressed in HEK293 cells. Production of the mosquito receptor transcripts in transiently-transfected cells was first verified using RT-PCR (Figure S4). Increases of intracellular cAMP were detected in cells transiently expressing either AaDOP1 [2.7±0.6 fold (n = 3)] or AaDOP2 [48±14 fold (n = 3)] in response to a single dose of dopamine (10 µM) (Figure S5). No significant increase in cAMP was observed in the mock transfected cells (empty pcDNA3.1+ vector). For cells transiently expressing AaDOP1, relatively high levels of constitutive activity were observed (17.6±2.4 fold greater than in mock transfected cells) as compared to AaDOP2 (1.83±0.93 fold greater than in mock transfected cells).
Subsequently, dose-response curves for seven different biogenic amines were generated using HEK-CRELuc cells stably expressing either AaDOP1 or AaDOP2 (Figure 4; Table 1). Again, dopamine stimulated both receptors, with EC50 values determined at 3.1±1.1 nM and 240±16.0 nM for AaDOP1 and AaDOP2, respectively (Figure 4A–B; Table 1). In addition, we observed activation of the AaDOP1 receptor by epinephrine (EC50 = 5.8±1.5 nM) and norepinephrine (EC50 = 760±180 nM) (Table 1). Conversely, no significant stimulation was observed for the AaDOP2 receptor by epinephrine or norepinephrine (Table 1). Neither receptor was stimulated by histamine, octopamine, serotonin, or tyramine (EC50≥10 uM). The effects of known synthetic dopamine receptor agonists were also investigated (Figure 4C–D; Table 1). Considerable stimulation was observed for AaDOP1 with the agonists listed in their rank order of potency: DHX>SKF81297>SKF38393. In contrast, of the synthetic agonists tested here, only treatment with DHX resulted in significant dose-dependent activation of AaDOP2. The addition of the D1 dopamine receptor antagonist SCH23390 (10 µM) robustly inhibited the dopamine-mediated stimulation of both AaDOP1 and AaDOP2 (Figure 4E).
The mosquito receptors were stably expressed in HEK 293-CRELuc cells for dose-response assays and determination of EC50 values (shown in Table 1). A, C: AaDOP1, B, D: AaDOP2. Representative curves for A, B: biogenic amines; C, D: synthetic dopamine receptor agonists; E: Inhibitory effect of 10 µM SCH23390 in the presence of 1 µM dopamine (n = 4) shown for both mosquito dopamine receptors. ** p<0.01; *** p<0.001; F: Dose-response curve of dopamine for AaDOP2 in the absence or presence of 10 µM SCH23390 used to identify an appropriate “signal window” for chemical library screening. The concentration of dopamine selected for screening (300 nM) is indicated with a box. CPS = counts per second; M = molarity.
Screening of Aadop2 against the LOPAC1280 library
We selected the AaDOP2 receptor for an antagonist screen of the LOPAC1280 library because of its low constitutive activity and strong dopamine response compared to background (approximately 10-fold) (Figure 4B,D). Using dose-response studies, it was determined that 300 nM dopamine alone and in combination with 10 µM SCH23390 created a suitable “signal window” for identification of AaDOP2 antagonists (Figure 4F). A “checkerboard analysis” using these conditions and assuming four replicates in the screen generated a Z-factor of 0.5±0.1 (n = 3), indicating that the assay was suitable for antagonist screening.
The criterion for “hit” detection was established relative to the control antagonist (SCH23390 response +3 standard deviations), such that only those compounds that inhibited the dopamine response by at least 81% were considered (Table 2). Based on this, our screen identified 51 potential antagonists of the AaDOP2 receptor (complete screen results provided in Table S4). These compounds were partitioned into seven different classes based on their known biochemical interactions with mammalian molecular targets that included dopamine receptor antagonists (20), serotonin (6), histamine (2), and acetylcholine receptor ligands (1), biogenic amine uptake inhibitors (9), protein kinase modulators (6), and miscellaneous chemistries such as cell cycle regulators and apoptosis inhibitors (7).
Ten “hit” compounds (amitriptyline hydrochloride, (±)-butaclamol hydrochloride, clozapine, doxepin hydrochloride, cis-(Z)-flupenthixol dihydrochloride, methiothepin maleate, mianserin hydrochloride, niclosamide, piceatannol, and resveratrol), in addition to SCH23390 were selected for screen validation assays. These compounds were tested for their activity in cAMP accumulation assays to control for potential “off-target” effects (i.e. chemistries that affect the CRELuc reporter system). Seven of these compounds were potent antagonists of the AaDOP2 receptor, as shown by the dose-dependent reduction of cAMP accumulation relative to the dopamine-stimulated control (Table 3, Figure 5). Three of the compounds (i.e. niclosamide, piceatannol, and resveratrol) showed no significant antagonistic effects against AaDOP2 in the cAMP accumulation experiments, having IC50 values ≥10 µM.
Direct cAMP accumulation assays were used for dose-response assays and determination of IC50 values for SCH23390 (antagonist control) and seven AaDOP2 antagonists (shown in Table 3) identified in the chemical library screen.
Confirmation and secondary in vitro assays.
Selected hit compounds were also tested against the human D1 receptor (hD1) to allow for comparisons of relative potency between species (Table 3). These experiments clearly indicated a unique pharmacology of AaDOP2 compared to hD1 with divergent rank order functional potencies that showed no significant correlation (R2<0.15). For example, the prototypical mammalian D1 antagonist, SCH23390 was greater than 3000-fold more selective for hD1 than AaDOP2. In contrast, the data also revealed that two structurally-related tricyclic antidepressants (i.e. amitriptyline and doxepin) had more than 30-fold selectivity for AaDOP2 when compared to hD1. These observations suggest that the significant differences between these receptors could be exploited for the development of AaDOP2-selective compounds.
In vivo Ae. aegypti bioassays
The toxicity of the AaDOP2 antagonist screen hits amitriptyline and doxepin was assessed in Ae. aegypti larval bioassays. These chemistries were selected due to their relatively higher potency at AaDOP2 compared to hD1 (Table 3). Single dose-point assays at 400 µM effective concentration of drug revealed that amitriptyline (93% average mortality) and doxepin (72% average mortality) each caused significant mortality (p<0.05) 24 hours post-treatment relative to the water control (0% mortality) (Figure 6A), whereas no mortality was observed for SCH23390 during this timeframe (data not shown). In addition, dose-response experiments were conducted for amitriptyline, which caused a rapid and high mortality effect in the single-point assays. The toxicity of amitriptyline was dose-dependent, and the LC50 and LC90 values were determined at 78 µM and 185 µM, respectively (Figure 6B).
A: Ae. aegypti larval bioassay showing toxicity of amitriptyline and doxepin at a single dose point (400 µM) compared to the water control; Ami = amitriptyline, Dox = Doxepin; * indicates p<0.05; B: Ae. aegypti larval bioassay involving amitriptyline in a dose-response format (25 µM–400 µM).
This work provides the first detailed investigation into the molecular and pharmacological properties of D1-like dopamine receptors, AaDOP1 and AaDOP2, from the mosquito vector of dengue and yellow fever, Ae. aegypti, and the development of a cell-based screen assay to discover antagonists of AaDOP2. Our study employed a novel pipeline utilizing a “genome-to-lead” approach for the discovery of new chemistries for vector control. This research establishes a basis for improving understanding of mosquito dopaminergic processes in vivo and for chemical screening of these and other receptors characterized in arthropod vectors of human disease, such as in the Lyme disease tick, I. scapularis , . To our knowledge, Lee and Pietrantonio  have published the only other study involving the functional characterization of a biogenic amine-binding GPCR in mosquitoes that was focused on a Gαs-coupled serotonin receptor in Ae. aegypti. Furthermore, ligands of only four other cloned GPCRs have been pharmacologically verified in mosquitoes, including those that target an adipokinetic hormone receptor, a corazonin receptor, a crustacean cardioactive peptide receptor , and an adipokinetic/corazonin-related peptide receptor in the malaria mosquito, A. gambiae .
Typically, insects possess three different dopamine receptors including two D1-like receptors and a single D2-like receptor . Here, RT-PCR data were used to validate the two mosquito D1-like dopamine receptor gene models ; this enabled confirmation of intron/exon boundaries and prediction of the complete protein coding regions needed prior to heterologous expression studies. A putative D2-like dopamine receptor gene (AaDOP3) was also identified in Ae. aegypti  although this receptor has not yet been functionally characterized. The RT-PCR studies also demonstrated that transcripts for both D1-like dopamine receptor genes were detectable in each developmental stage of Ae. aegypti, suggesting the importance of these receptors throughout the mosquito life cycle. Much progress has been made in determining the life-stage and tissue-specific expression dynamics of the orthologous dopamine receptors in D. melanogaster , , , , , , A. mellifera , , , and most recently in the Lyme disease tick, I. scapularis . Our research will support future complementary studies needed to localize expression of these dopamine receptors in mosquito tissues to gain further insight toward their neurophysiological roles.
The AaDOP1 and AaDOP2 amino acid sequences were compared and analyzed to identify conserved as well as unique features of the receptors. Several characteristics typically associated with biogenic amine-binding GPCRs were evident, including aspartate residues in TM II and TM III that are thought to interact with the amine moieties of catecholamines . The conserved serine residues in TM V and aromatic residues in TM V and VI are also potentially important for ligand interaction , . In both receptors, the conceptual cytoplasmic region of TM III contained the conserved “DRY” motif associated with G protein-coupling , , and a pair of cysteine residues were located in the extracellular loops I and II that may form a disulfide bond for protein stabilization , , . Interestingly, the divergent intracellular loop III was predicted to be almost twice as long in AaDOP2 (115 amino acids) than in AaDOP1 (62 amino acids), but the sizes of the carboxyl tail region were similar between these receptors. This corresponded well with the relative sizes of these features in the fruit fly and honeybee orthologs ; however, the significance of these characteristics is yet to be determined in the mosquito. Importantly, the AaDOP1 and AaDOP2 sequences were markedly different from the human D1-like dopamine receptor sequences. Although a modest level of amino acid identity (~50%) was observed between the TM domains, the N- and C-termini and extracellular and intracellular loop regions were highly divergent (data not shown). These differences suggest that there exists potential for identifying chemistries that are mosquito-specific and, importantly, do not interfere with dopaminergic functioning in humans.
Heterologous expression experiments conducted in HEK293 cells provided experimental evidence that the Ae. aegypti receptors are functional D1-like dopamine receptors. We measured significant increases in cAMP accumulation following dopamine treatment of cells transiently expressing either AaDOP1 or AaDOP2, suggesting that both receptors couple to Gαs proteins. This effect was further substantiated in cell lines stably co-expressing either of these receptors and the CRELuc reporter system, as measured by an increase in luciferase activity following dopamine treatment. Future research is needed to determine if these receptors operate through multiple cellular signaling mechanisms, such as was shown for the D. melanogaster dopamine receptor involved with both cAMP and calcium signaling .
The stably transformed cell lines were used to compare the pharmacological properties of AaDOP1 and AaDOP2 in response to seven different biogenic amines. For dopamine, we measured EC50 values in the nanomolar range for both AaDOP1 (3.1±1.1 nM) and AaDOP2 (240±16 nM). However, there were differences in the responses of these receptors to the other biogenic amines. AaDOP2 was activated only with dopamine, whereas AaDOP1 was stimulated by dopamine, epinephrine, and to a lesser extent, norepinephrine. These results were similar to those reported for the orthologous dopamine receptors in the tick I. scapularis , . Another difference between AaDOP1 and AaDOP2 was observed regarding constitutive activity. In both transient and stable expression experiments, the AaDOP1 receptor exhibited significant constitutive activity, as determined by the elevated levels of cAMP detected in the absence of a receptor agonist, whereas AaDOP2 did not. Such constitutive activity was also reported for the D1-like dopamine receptors AmDOP1 of A. mellifera , CeDOP1 from the nematode Caenorhabditis elegans , Isdop1 of I. scapularis , and the human D5 receptor . Seifert and Wenzel-Seifert  proposed that constitutive activity of a GPCR may enable the maintenance of basal neuronal activity, although evidence is needed to support such activity for AaDOP1 in vivo.
The pharmacological properties of AaDOP1 and AaDOP2 were further explored by testing their responses to synthetic dopamine receptor agonists and antagonists. Both receptors were strongly stimulated by the agonist DHX; however, only AaDOP1 significantly responded to the well characterized D1 agonists SKF81297 and SKF38393. This differential response to the SKF compounds was also observed for the orthologous D1-like dopamine receptors in the tick I. scapularis . Interestingly, neither of the D. melanogaster D1-like dopamine receptors was strongly stimulated by SKF38393 , . Both AaDOP1 and AaDOP2 were inhibited by the antagonist SCH23390, as were the tick D1-like receptors . This contrasted with the lack of significant inhibition reported by SCH23390 for D-dop1 in the fruit fly  and DOP1 of the honeybee . Given the limited number of drugs that have been tested against these receptors, to date, these differential pharmacological responses provide further evidence that it may be possible to discover chemistries that operate specifically at the mosquito dopamine receptors.
Our over-arching goal was to develop a pipeline to identify lead chemistries active at biogenic-amine binding GPCRs in vector arthropods. Broadly speaking, we define a lead chemistry as any molecule, or its analog or derivative, with potential for insecticide development. In our study, this refers to any molecule identified by screening and subsequently confirmed in a variety of “hit-to-lead” assays. The LOPAC1280 library was chosen for our pilot screen because it is enriched with chemistries that influence dopaminergic processes and includes other GPCR-binding ligands. We hypothesized that chemistries that antagonize these dopamine receptors may possess insecticidal properties. Precedent for this concept stems from pest management successes associated with the use of phenylpyrazoles (e.g. Fipronil) and cyclodienes, which block GABA-gated chloride channels and have highly insecticidal properties , . This notion was pursued using HEK293 cells stably expressing AaDOP2 because this receptor has a robust response to dopamine and a low constitutive activity, which are properties that aid interpretation of screen data. Our initial screen was directed at the identification of AaDOP2 antagonists; the success of this experiment justifies expanded screening to explore the antagonist chemical “space”, and with assay modification, screens to detect agonists active at this receptor. Moreover, development of the AaDOP1 assay would enable comparative screens against LOPAC1280 chemistries.
Of the 51 hit AaDOP2 antagonists identified in the LOPAC1280 library, 20 (39%) are known antagonists of mammalian dopamine receptors. A majority of these chemistries fall into the benzodiazepine, phenothiazine, or thioxanthene classes that in other systems are known to bind other biogenic amine receptors. Included were ligands selective for D1- and D2-like dopamine receptors in mammalian systems, as well as several non-dopamine receptor selective compounds such as (±)-butaclamol, cis-(Z)-flupenthixol, and the atypical antipsychotic, clozapine. These three compounds were tested in a dose-response format for their ability to inhibit dopamine-stimulated cAMP accumulation. The IC50 values demonstrated the following rank order of potency clozapine>cis-flupenthixol>butaclamol. The next largest grouping of identified compounds includes inhibitors of the biogenic amine transporters (9 compounds, 18%). Several serotonin receptor antagonists (6 compounds, 12%) were identified as well. Follow-up dose response studies with selected chemistries from the identified transport inhibitors and serotonin antagonists (i.e. methiothepin, mianserin, amitriptyiline, and doxepin) revealed that these compounds were potent antagonists at the AaDOP2 receptor and were much more potent than the prototypical D1 antagonist, SCH23390 (Table 3). The antagonistic activity of these ligands is not completely surprising; the National Institute of Mental Health's Psychoactive Drug Screening Program (NIMH-PDSP) database reports Ki values for the human D1-like dopamine receptors at 80–900 nM (http://pdsp.med.unc.edu/). However, these observations, combined with the dopamine antagonist screen results, indicate that well studied and clinically used compounds could be used to target invertebrate GPCRs. In fact, a number of the chemistries identified in our screen have been used in humans for decades, suggesting the possibility of “drug repurposing” as insecticides. Further precedent for the concept of insect-specific chemistries can be drawn from the fact that a number of insecticides (e.g., pyrethroids and fipronil) are considerably more selective at invertebrate as opposed to mammalian targets . The screen also identified multiple protein kinase modulators and several agents that regulate germane cellular functions that presumably inhibit the CRE response via non-AaDOP2 mechanisms. Support for this hypothesis was demonstrated in the direct measurement of cAMP accumulation experiments, where resveratrol, pieacetannol, and niclosamide each lacked activity. The remaining three “hit” compound classes included antagonists of either histamine or muscarinic acetylcholine receptors, and this likely reflects the lack of receptor selectivity for these ligands.
The LOPAC1280 library includes several known antagonists of mammalian dopamine receptors that did not qualify as hits in our screen. In part, this can be explained by the fact that we used a highly stringent cut-off to signify antagonistic activity at AaDOP2. Had we reduced the stringency to select for hits with an antagonistic effect equivalent to that of SCH23390+6 standard deviations (69% inhibition), our screen would have returned an additional 13 hit chemistries, including compounds predicted to have a modest antagonistic effect at AaDOP2 and those that are more selective for D2-like dopamine receptors. Considering the substantial divergence between the mosquito and human D1-like dopamine receptor sequences, there is a strong possibility that a subset of the “non-hit” dopamine receptor antagonists are not active at the mosquito receptor. In support of this, the prototypical mammalian D1 antagonist, SCH23390, was greater than 3000-fold more selective for hD1 than AaDOP2. Although our comparison data set is limited to only eight compounds, these experiments suggest a very divergent pharmacology between these human and mosquito dopamine receptors. Thus, our study provides a foundation for subsequent comparative pharmacological analyses of the mosquito and human dopamine receptors.
Analyses involving a small subset of compounds revealed a correlation between our in vitro and in vivo data. The AaDOP2 antagonist screen hits, amitriptyline and doxepin, caused significant lethality in the mosquito bioassay. Our finding that these drugs each have a relatively higher potency at the mosquito dopamine receptor than at the human dopamine receptor (hD1) has implications for the identification of arthropod-selective chemistries. Drugs with minimal or no impact on the neurological functioning of humans or other vertebrate species are particularly desirable as prospects for insecticide development. Conversely, SCH23390, which is active at AaDOP2 only in the micromolar range and was several fold more selective for hD1 in cAMP assays, did not cause significant mortality at 24 hr.
The success of this initial chemical library screen in identifying new mosquitocidal chemical leads justifies the pursuit of an expanded high-throughput screening effort involving thousands or hundreds of thousands of chemistries against mosquito dopamine receptors. Our platform is also amenable for the screening of agonist chemistries active at these mosquito dopamine receptors, as well as for Gαs-coupled biogenic amine targets of other vector arthropods, and also could be modified to screen Gαi/o-coupled receptors . Importantly, the identification of lead AaDOP2 receptor antagonistic chemistries provides a basis for investigating the effect of these or related compounds on mosquito dopaminergic processes in vivo . Follow-up research is needed to determine the precise mechanism(s) of amitriptyline- and doxepin-induced mortality in Ae. aegypti. Further work is also needed to determine if these chemistries and associated derivatives or analogs identified by chemical screens possess the properties desired of an insecticide (e.g. bioavailability, in vivo potency/toxicity, suitable half-life, lack of effects on non-target organisms, suitability for synthesis and formulation). Molecular modeling of three dimensional GPCR structures and their binding capabilities, as reported for an adipokinetic hormone receptor in A. gambiae  and a tyramine receptor in the moth Plodia interpunctella , may facilitate in silico chemical screening  and ligand-receptor studies that permit the design or refinement of lead molecules active at mosquito GPCRs.
Historically, multiple neuroactive processes in arthropods have been exploited for pest control using insecticides such as chlorinated hydrocarbons, organophosphates, methylcarbamates, pyrethroids, amidines, and phenylpyrazoles . Resistance involving each of these classes (the vast majority of which operate by affecting ion channels and neurotransmitters) has been documented. The development of new mode-of-action insecticides could improve our arsenal against mosquito populations that have developed resistance to existing chemical formulations . We suggest that the two dopamine receptors characterized here, as well as other biogenic amine-binding GPCRs , , represent promising targets for new insecticide research, due to their presumably central roles in insect neurobiology. This “proof-of-concept” study sets the stage for target-specific approaches for vector control. Such efforts, in parallel with activities of organizations such as the Innovative Vector Control Consortium, may help to realize the goal of delivering new insecticides for reduction of vector-borne diseases .
Gel electrophoresis for non-quantitative RT-PCR of Aedes aegypti Aadop1 and Aadop2. A: Aadop1 amplified with primers Aadop_1F/1R (224 bp amplicon), B: Aadop2 amplified with primers Aadop2_Full_F/R (1,425 bp amplicon). Transcripts were detected for both dopamine receptors in each developmental stage of the mosquito and both adult sexes. As expected, no amplification products were detected in the negative control, which contained identical reagents as the other reactions but lacked an RNA template. Abbreviations: (M) DNA size marker (HyperLadder I, Bioline USA Inc., Randolph, MA); (E) egg; (L) larva; (P) pupa; (AF) adult female; (AM) adult male; (C) negative control.
Gene models for Aedes aegypti Aadop1 and Aadop2. A: Aadop1, B: Aadop2. Exons (E) are shown with gray bars, and introns with solid black lines. Numbers above the box/line indicate the size of exon/intron in base pairs (bp), respectively. The putative transmembrane domains (I–VII) are shown with black boxes along the exons. The gene structures of Aadop1 and Aadop2 include three and two introns, respectively, which is consistent with other characterized insect dopamine receptor genes that also contain introns , but is in contrast with the single exon gene structures reported for the two D1-like receptor genes in humans ,  and the Lyme disease tick, I. scapularis . The genomic supercontigs on which Aadop1 and Aadop2 reside have not yet been linked to chromosomal positions , so their relative genome organization cannot yet be compared with other insects. However, in A. gambiae the predicted orthologs of Aadop1 and Aadop2 are positioned on chromosome 2R (GPRDOP1: AGAP004613) and the X chromosome (GPRDOP2: AGAP000667) .
Alignment of transmembrane domains of Aedes aegypti AaDOP1 and AaDOP2 and other D1-like receptors. Aligned receptor amino acid sequences include each of the two D1-like receptors reported in Drosophila melanogaster (D-Dop1; DopR99B/DAMB) , , , , Apis mellifera (AmDOP1; AmDOP2) , Ixodes scapularis (Isdop1; Isdop2) , , and Homo sapiens (HsD1, HsD5) , . Amino acids included in the alignment were related to the TM regions predicted for D. melanogaster , . Shaded amino acids designate residues conserved among each of the aligned TM domain sequences.
Expression of Aedes aegypti Aadop1 and Aadop2 in transiently-transfected HEK 293 cells. Gel electrophoresis shows PCR and RT-PCR amplification of Aedes aegypti A: Aadop1and B: Aadop2 using primers Aadop1_1F/2R (amplicon = 1058 bp) and Aadop2_FullF/FullR (1425 bp), respectively. Abbreviations: (M) DNA size marker (HyperLadder I, Bioline USA Inc., Randolph, MA); lanes under the heading “PCR” include controls for DNA contamination in the RNA preparation: (−) no DNA template; (+) A: DNA construct pcDNA3.1+/Aadop1 and B: DNA construct pcDNA3.1+/Aadop2; (V) mRNA from cells transfected with empty vector pcDNA3.1; (C) mRNA from cells transfected with construct A: pcDNA3.1+/Aadop1 and B: pcDNA3.1+/Aadop2. Lanes under the heading “RT-PCR” show mRNA transcript detection experiments; (−) no template mRNA; (+) mRNA from adult female Ae. aegypti (non-specific amplification products were eliminated with gel purification); (V) mRNA from cells transfected with empty vector pcDNA3.1; (C) mRNA from cells transfected with construct A: pcDNA3.1+/Aadop1 and B: pcDNA3.1+/Aadop2.
Response of AaDOP1 and AaDOP2 following dopamine treatment in transiently-transfected HEK cells. Significant responses to dopamine were observed for both AaDOP1 and AaDOP2, relative to basal conditions (p<0.05).
Primer pairs and experimental conditions used in RT-PCR analysis of Aadop1 and Aadop2 transcripts.
Summary of selected amino acid features of Aedes aegypti Aa DOP1 and Aa DOP2.
Comparison of transmembrane domains of A. aegypti Aa DOP1 and Aa DOP2 and related D1-like receptors.
Results of the Aedes aegypti Aa DOP2 antagonist screen of the LOPAC1280 library.
The authors thank D. Nichols and E. Barker for providing selected chemicals used in pharmacological and in vivo assays, J. Conley for assistance in assay automation, and M. Scharf for technical advice regarding bioassays and reviewing this manuscript prior to submission. We thank C. Buck for coordination of chemical library screening.
Conceived and designed the experiments: JMM KFKE VJW CAH. Performed the experiments: JMM KFKE LVA EEG-K GIG-C TFB. Analyzed the data: JMM KFKE LVA EEG-K GIG-C TFB VJW CAH. Contributed reagents/materials/analysis tools: VJW CAH. Wrote the paper: JMM KFKE VJW CAH.
- 1. Hemingway J, Ranson H (2000) Insecticide resistance in insect vectors of human disease. Annu Rev Entomol 45: 371–391.
- 2. Hemingway J, Beaty BJ, Rowland M, Scott TW, Sharp BL (2006) The Innovative Vector Control Consortium: improved control of mosquito-borne diseases. Trends Parasitol 22: 308–312.
- 3. Greenwood BM, Fidock DA, Kyle DE, Kappe SH, Alonso PL, et al. (2008) Malaria: progress, perils, and prospects for eradication. J Clin Invest 118: 1266–76.
- 4. Strader CD, Fong TM, Graziano MP, Tota MR (1995) The family of G protein-coupled receptors. FASEB J 9: 745–754.
- 5. Gether U (2000) Uncovering molecular mechanisms involved in activation of G protein-coupled receptors. Endocr Rev 21: 90–113.
- 6. Filmore D (2004) It's a GPCR world. Modern Drug Disc 7: 24–28.
- 7. Gamo F-J, Sanz LM, Vidal J, de Cozar C, Alvarez E, et al. (2010) Thousands of chemical starting points for antimalarial lead identification. Nature 465: 305–312.
- 8. Chen AC, He H, Davey RB (2007) Mutations in a putative octopamine receptor gene in amitraz-resistant cattle ticks. Vet Parasitol 148: 379–383.
- 9. Hill CA, Fox AN, Pitts RJ, Kent LB, Tan PL, et al. (2002) G protein-coupled receptors in Anopheles gambiae. Science 298: 176–178.
- 10. Nene V, Wortman JR, Lawson D, et al. (2007) Genome sequence of Aedes aegypti, a major arbovirus vector. Science 316: 1718–1723.
- 11. Hauser F, Cazzamali G, Williamson M, Blenau W, Grimmelikhuijzen JP (2006) A review of neurohormone GPCRs present in the fruitfly Drosophila melanogaster and the honey bee Apis mellifera. Prog Neurobiol 80: 1–19.
- 12. Kebabian JW, Calne DB (1979) Multiple receptors for dopamine. Nature 277: 93–96.
- 13. Yellman C, Tao H, He B, Hirsh J (1997) Conserved and sexually dimorphic behavioral responses to biogenic amines in decapitated Drosophila. Proc Natl Acad Sci U S A 94: 4131–4136.
- 14. Draper I, Kurshan PT, McBride E, Jackson FR, Kopin AS (2007) Locomotor activity is regulated by D2-like receptors in Drosophila: an anatomic and functional analysis. Dev Neurobiol 67: 378–393.
- 15. Mustard JA, Pham PM, Smith BH (2010) Modulation of motor behavior by dopamine and the D-1 like receptor AmDOP2 in the honey bee. J Insect Physiol 56: 422–430.
- 16. Kume K, Kume S, Park SK, Hirsh J, Jackson FR (2005) Dopamine is a regulator of arousal in the fruit fly. J Neurosci 25: 7377–7384.
- 17. Kim YC, Lee HG, Han KA (2007) D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila. J Neurosci 27: 7640–7647.
- 18. Riemensperger T, Isabel G, Coulom H, Neuser K, Seugnet L, et al. (2011) Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proc Natl Acad Sci U S A 108: 834–839.
- 19. Ali DW (1997) The aminergic and peptidergic innervation of insect salivary glands. J Exp Biol 200: 1941–1949.
- 20. Sauer JR, Essenberg RC, Bowman AS (2000) Salivary glands in Ixodid ticks: control and mechanism of secretion. J Insect Physiol 46: 1069–1078.
- 21. Andersen JP, Schwartz A, Gramsbergen JB, Loeschcke V (2006) Dopamine levels in the mosquito Aedes aegypti during adult development, following blood feeding and in response to heat stress. J Insect Phys 52: 1163–1170.
- 22. Ferdig MT, Taft AS, Smartt CT, Lowenberger CA, Li J, et al. (2000) Aedes aegypti dopa decarboxylase: gene structure and regulation. Insect Mol Biol 9: 231–239.
- 23. Johnson JK, Li J, Christensen BM (2001) Cloning and characterization of a dopachrome conversion enzyme from the yellow fever mosquito, Aedes aegypti. Insect Biochem Mol Biol 31: 1125–1135.
- 24. Huang C-Y, Christensen BM, Chen C-C (2005a) Role of dopachrome conversion enzyme in the melanization of filarial worms in mosquitoes. Insect Mol Biol 14: 675–682.
- 25. Huang C-Y, Chou S-Y, Bartholomay LC, Christensen BM, Chen C-C (2005b) The use of gene silencing to study the role of dopa decarboxylase in mosquito melanization reactions. Insect Mol Biol 14: 237–244.
- 26. Hodgetts RB, O'Keefe SL (2006) Dopa decarboxylase: A model gene-enzyme system for studying development, behavior, and systematics. Annu Rev Entomol 51: 259–284.
- 27. Moreira LA, Ye YH, Turner K, Eyles DW, McGraw EA, et al. (2011) The wMelPop strain of Wolbachia interferes with dopamine levels in Aedes aegypti. Parasites and Vectors 4: 28.
- 28. Pratt S, Pryor SC (1986) Dopamine- and octopamine-sensitive adenylate cyclase in the brain of adult Culex pipiens mosquitoes. Cellular Mol Neurobiol 6: 325–329.
- 29. Lawson D, Arensburger P, Atkinson P, Besansky NJ, Bruggner RV, et al. (2008) VectorBase: a data resource for invertebrate vector genomics. Nucleic Acids Res 37: Database issue, D583–587.
- 30. Gotzes F, Balfanz S, Baumann A (1994) Primary structure and functional characterization of a Drosophila dopamine receptor with high homology to human D1/5 receptors. Receptor Chan 2: 131–141.
- 31. Feng G, Hannan F, Reale V, Hon YY, Kousky CT, et al. (1996) Cloning and functional characterization of a novel dopamine receptor from Drosophila melanogaster. J Neurosci 16: 3925–3933.
- 32. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, et al. (2000) Artemis: sequence visualization and annotation. Bioinformatics 16: 944–945.
- 33. Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, et al. (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res 31: 3497–3500.
- 34. Swofford DL (2001) Phylogenetic analysis using parsimony (*and other methods) Version 4. Sinauer Assoc, Sunderland, MA.
- 35. Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16: 10881–10890.
- 36. Meyer JM, Ejendal KFK, Watts VJ, Hill CA (2011) Molecular and pharmacological characterization of two D1-like dopamine receptors in the Lyme disease vector, Ixodes scapularis. Insect Biochem Mol Biol 41: 563–571.
- 37. Przybyla JA, Cueva JP, Chemel BR, Hsu KJ, Riese DJ, et al. (2009) Comparison of the enantiomers of (±)-doxanthrine, a high efficacy full dopamine D1 receptor agonist, and a reversal of enantioselectivity at D1 versus alpha2C adrenergic receptors. Eur Neuropsychopharmacol 19: 138–146.
- 38. Zhang J-H, Chung TDY, Oldenburg KR (1999) Statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4: 67–73.
- 39. Sunahara RK, Niznik HB, Weiner DM, Stormann TM, Brann MR, et al. (1990) Human dopamine D1 receptor encoded by an intronless gene on chromosome 5. Nature 347: 80–83.
- 40. Sugamori KS, Demchyshyn LL, McConkey F, Forte MA, Niznik HB (1995) A primordial dopamine D1-like adenylyl cyclase-linked receptor from Drosophila melanogaster displaying poor affinity for benzazepines. FEBS Lett 362: 131–138.
- 41. Mustard JA, Blenau W, Hamilton IS, Ward VK, Ebert PR, et al. (2003) Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity. Mol Brain Res 12: 67–77.
- 42. Šimo L, Koči J, Žitňan D, Park Y (2011) Evidence for D1 dopamine receptor activation by a paracrine signal of dopamine in tick salivary glands. PLoS One 6: e16158.
- 43. Mustard JA, Beggs KT, Mercer AR (2005) Molecular biology of the invertebrate dopamine receptors. Arch Insect Biochem Physiol 59: 103–117.
- 44. Han K-A, Millar NS, Grotewiel MS, Davis RL (1996) DAMB, a novel dopamine receptor expressed specifically in Drosophila mushroom bodies. Neuron 16: 1127–1135.
- 45. Han K-A, Millar NS, Davis RL (1998) A novel octopamine receptor with preferential expression in Drosophila mushroom bodies. J Neurosci 18: 3650–3658.
- 46. Grohmann L, Blenau W, Erber J, Ebert PR, Strunker T, et al. (2003) Molecular and functional characterization of an octopamine receptor from honeybee (Apis mellifera) brain. J Neurochem 86: 725–735.
- 47. Lee D-W, Pietrantonio PV (2003) In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves. Insect Mol Biol 12: 561–569.
- 48. Belmont M, Cazzamali G, Williamson M, Hauser F, Grimmelikhuizen CJP (2006) Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae. Biochem Biophys Res Comm 344: 160–165.
- 49. Hansen KK, Stafflinger E, Schneider M, Hauser F, Cazzamali G, et al. (2010) Discovery of a novel insect neuropeptide signaling system closely related to the insect adipokinetic hormone and corazonin hormonal systems. J Biol Chem 285: 10736–10747.
- 50. Kim Y-C, Lee H-G, Seong C-S, Han K-A (2003) Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster. Gene Exp Pat 3: 237–245.
- 51. Blenau W, Erber J, Baumann A (1998) Characterization of a dopamine receptor from Apis mellifera: Cloning, functional expression, pharmacology, and mRNA localization in the brain. J Neurochem 70: 15–23.
- 52. Kokay IC, Ebert PR, Kirchhof BS, Mercer AR (1999) Distribution of dopamine receptors and dopamine receptor homologs in the brain of the honey bee, Apis mellifera L. Microscopy Res Tech 44: 179–189.
- 53. Blenau W, Baumann A (2001) Molecular and pharmacological properties of insect biogenic amine receptors: Lessons from Drosophila melanogaster and Apis mellifera. Arch Insect Biochem Physiol 48: 13–38.
- 54. Humphries MA, Mustard JA, Hunter SJ, Mercer A, Ward V, et al. (2003) Invertebrate D2 type dopamine receptor exhibits age-based plasticity of expression in the mushroom bodies of the honeybee brain. J Neurobiol 55: 315–330.
- 55. Strader CD, Sigal IS, Candelore MR, Rands E, Hill WS, et al. (1988) Conserved aspartic acid residue-79 and residue-113 of the beta-adrenergic receptor have different roles in receptor function. J Biol Chem 263: 10267–10271.
- 56. Strader CD, Candelore MR, Hill WS, Sigal IS, Dixon RAF (1989) Identification of two serine residues involved in agonist activation of the beta-adrenergic receptor. J Biol Chem 264: 13572–13578.
- 57. Pollock NJ, Manelli AM, Hutchins CW, Steffey ME, MacKenzie RG, et al. (1992) Serine mutations in transmembrane-V of the dopamine D1-receptor affect ligand interactions and receptor activation. J Biol Chem 267: 17780–17786.
- 58. Dixon RAF, Sigal IS, Candelore MR, Register RB, Scattergood W, et al. (1987) Structural features required for ligand binding to the β-adrenergic receptor. EMBO J 6: 3269–3275.
- 59. Fraser CM, Chung FZ, Wang CD, Venter JC (1988) Site-directed mutagenesis of human β-adrenergic receptors: substitution of aspartic acid-130 by asparagine produces a receptor with high-affinity agonist binding that is uncoupled from adenylate cyclase. Proc Natl Acad Sci U S A 85: 5478–5482.
- 60. Karnik SS, Sakmar TP, Chen HB, Khorana HG (1988) Cysteine residues 110 and 187 are essential for the formation of the correct structure in bovine Rhodopsin. Proc Natl Acad Sci U S A 85: 8459–8463.
- 61. Fraser CM (1989) Site-directed mutagenesis of β-adrenergic receptors: identification of conserved cysteine residues that independently affect ligand binding and receptor activation. J Biol Chem 264: 9266–9270.
- 62. Reale V, Hannan F, Hall LM, Evans PD (1997) Agonist-specific coupling of a cloned Drosophila melanogaster D1-like dopamine receptor to multiple second messenger pathways by synthetic agonists. J Neurosci 17: 6545–6554.
- 63. Sanyal S, Wintle RF, Kindt KS, Nuttley WM, Arvan R, et al. (2004) Dopamine modulates the plasticity of mechanosensory responses in Caenorhabditis elegans. EMBO J 23: 473–482.
- 64. Tiberi M, Caron MG (1994) High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype. J Biol Chem 269: 27925–27931.
- 65. Seifert R, Wenzel-Seifert K (2002) Constitutive activity of G protein-coupled receptors: cause of disease and common property of wild-type receptors. Naunyn-Schmiedeberg's Arch Pharmacol 366: 381–416.
- 66. Bloomquist JR (1996) Ion channels as targets for insecticides. Ann Rev Entomol 41: 163–190.
- 67. Casida JE, Quistad GB (1998) Golden age of insecticide research: past, present or future? Annu Rev Entomol 43: 1–16.
- 68. Raymond-Delpech V, Matsuda K, Sattelle B, Rauh JJ, Sattelle DB (2005) Ion channels: molecular targets of neuroactive insecticides. Invert Neurosci 5: 119–133.
- 69. Missale C, Nash SR, Robinson SW, Jaber M, Caron MG (1998) Dopamine receptors: from structure to function. Physiol Rev 78: 189–225.
- 70. Pridgeon JW, Becnel JJ, Clark GG, Linthicum KJ (2009) A high-throughput screening method to identify potential pesticides for mosquito control. J Med Entomol 46: 335–341.
- 71. Mugumbate G, Jackson GE, van der Spoel D (2010) Open conformation of adipokinetic hormone receptor from the malaria mosquito facilitates hormone binding. Peptides 32: 553–559.
- 72. Hirashima A, Eiraku T, Kuwano E, Eto M (2004) Comparative receptor surface analysis of agonists for tyramine receptor which inhibit sex-pheremone production in Plodia interpunctella. Comb Chem High Throughput Screen 8: 589–594.
- 73. Senderowitz H, Marantz Y (2009) G protein-coupled receptors: target-based in silico screening. Curr Pharm Des 15: 4049–4068.
- 74. Benting J, Leonhardt M, Lindell SD, Tiebes J (2005) The design, synthesis and screening of a muscarinic acetylcholine receptor targeted compound library. Comb Chem High Throughput Screen 8: 649–653.
- 75. Roeder T (2005) Tyramine and octopamine: ruling behavior and metabolism. Annu Rev Entomol 50: 447–477.
- 76. Sunahara RK, Guan HC, O'Dowd BF, Seeman P, Laurier LG, et al. (1991) Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1. Nature 350: 614–619.